Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants

Molecular and Cellular Biology

Volumn 16, Issue 4, 1996, Pages 1519-1526

  Errami, Abdellatif ^a,b   Smider, Vaughn ^c   Rathmell, W Kimryn ^c   He, Dong Ming ^d   Hendrickson, Eric A ^d   Zdzienicka, Małgorzata Z ^a,b,e   Chu, Gilbert ^c  

^a LEIDEN UNIVERSITY   (Netherlands)

^b JA Cohen Institute Interuniversity Research Institute for Radiopathology and Radiation Protection   (Netherlands)

^c STANFORD UNIVERSITY MEDICAL CENTER   (United States)

^d BROWN UNIVERSITY   (United States)

^e LEIDEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; DOUBLE STRANDED DNA;

ANIMAL CELL; ARTICLE; CELL MUTANT; CHINESE HAMSTER; CONTROLLED STUDY; DNA REPAIR; DNA STRAND BREAKAGE; NONHUMAN; PRIORITY JOURNAL; RADIOSENSITIVITY; X RAY;

EID: 0029928176     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.16.4.1519     Document Type: Article

References (55)

1. Biedermann, K. A., J. Sung, A. J. Giaccia, L. M. Tosto, and J. M. Brown. 1991. scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double strand break repair. Proc. Natl Acad. Sci. USA 88:1394-1397
2. Blunt, T., N. J. Finnie, G. E. Taccioli, G. C. M. Smith, J. Demengeot, T. M. Gottlieb, R. Mizuta, A. J. Varghese, F. W. Alt, P. A. Jeggo, and S. P. Jackson. 1995. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell 80:813-823.
3. Boubnov, N. V., K. T. Hall, Z. Willis, S. E. Lee, D. M. He, D. M. Benjamin, C. R. Pulaski, H. Band, W. Reeves, E. A. Hendrickson, and D. Weaver. 1995. Complementation of the ionizing radiation sensitivity, DNA end binding, and V(D)J recombination defects of double-strand break repair mutants by the p86 Ku autoantigen. Proc Natl. Acad. Sci. USA 92:890-894
4. Cai, Q. Q., A. Plet, J. Imbert, M. Lafage-Pochitaloff, C. Cerdan, and J. M. Blanchard. 1994. Chromosomal location and expression of the genes coding for Ku p70 and p80 in human cell lines and normal tissues. Cytogenet Cell Genet. 65:221-227.
5. Cao, Q., S. Pitt, J. Leszyk, and E. Baril. 1994. DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen. Biochemistry 33:8548-8557.
6. Chen, D. J., M. S. Park, E. Campbell, M. Oshimnra, P. Liu, Z. Ying, B. F. White, and M. J. Siciliano. 1992. Assignment of a human DNA double-strand break repair gene (XRCC5) to chromosome 2. Genomics 13:1088-1094.
7. Chomczynski, P., and N. Sacchi. 1987. Single-step method or RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem 162:156-159.
8. Collins, A. R. 1993. Mutant rodent cell lines sensitive to ultraviolet light, ionizing radiation and cross-linking agents: a comprehensive survey of genetic and biochemical characteristics. Mutat. Res. 293:99-118.
9. 8a. Corpet, F. 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16:10881-10890.
10. de Vries, E., W. van Driel, W. G. Bergsma, A. C. Arnberg, and P. C. van der Vliet. 1989. HeLa nuclear protein recognizing DNA termini and translocating on DNA forming a regular DNA-multimeric protein complex. J. Mol Biol. 208:65-78.
11. Falzon, M., and E. L. Kuff. 1992. The nucleotide sequence of a mouse cDNA encoding the 80 kDa subumt of the Ku (p70/p86) autoantigen. Nucleic Acids Res. 20:3784.
12. Friedberg, E. C., G. C. Walker, and W. Siede. 1995 DNA repair and mutagenesis. ASM Press. Washington, D.C
13. Fulop, G. M., and R. A. Phillips. 1990. The scid mutation in mice carnes a general defect in DNA repair. Nature (London) 347:479-482.
14. Getts, R. C., and T. D. Stamato. 1994 Absence of a Ku-like DNA end binding activity in the xrs double-strand DNA repair-deficient mutant. J Biol. Chem. 269:15981-15984
15. Gottlieb, T. M., and S. P. Jackson. 1993. The DNA-dependent protein kinase, requirement for DNA ends and association with Ku antigen. Cell 72: 131-142.
16. Graham, F. L., and A. J. van der Eb. 1973. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456-467.
17. Hafezparast, M., G. P. Kaur, M. Z. Zdzienicka, R. S. Athwal, A. R. Lehmann, and P. A. Jeggo. 1993. Subchromosomal localization of a gene (XRCC5) involved in double-strand break repair to the region 2q34-36. Somatic Cell Mol. Genet 19:413-421.
18. He, D. M., S. E. Lee, and E. A. Hendrickson. Restoration of X-ray and etoposide resistance, Ku-end binding activity and V(D)J recombination to the Chinese hamster sxi-3 mutant by a hamster Ku86 cDNA. Mutat. Res , in press
19. Hendrickson, E. A., X. Q. Qin, E. A. Bump, D. G. Schatz, M. Oettinger, and D. T. Weaver. 1991. A link between double-strand break-related repair and V(D)J recombination: the scid mutation. Proc. Natl Acad. Sci. USA 88: 4061-4065.
20. Itoh, M., K. Hamatani, K. Komatsu, R. Araki, K. Takayama, and M. Abe. 1993. Human chromosome-8 (p12-q22) complements radiosensitivity in the severe combined immune deficiency (scid) mouse Radiat. Res. 134:364-368.
21. Jackson, S. P. 1992. Regulating transcription activity by phosphorylation. Trends Cell Biol. 2:104-118.
22. Jeggo, P. A., M. Hafezparast, A. F. Thompson, B. C. Broughton, G. P. Kaur, M. Z. Zdzienicka, and R. S. Athwal. 1992. Localization of a DNA repair gene (XRCC5) involved in double-strand-break rejoining to human chromosome 2. Proc. Natl. Acad. Sci USA 89:6423-6427.
23. Jeggo, P. A., and R. Holliday. 1986. Azacytidine-induced reactivation of a DNA repair gene in Chinese hamster ovary cells. Mol. Cell. Biol. 6:2944-2949.
24. Kirschgessner, C., C. Patil, J. Evans, C. Cuomo, L. Fried, T. Carter, M. Oettinger, and J. M. Brown. 1995. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267:1178-1185.
25. Kirchgessner, C. U., L. M. Tosto, K. A. Biedermann, M. Kovacs, D. Araujo, E. E. Stanbridge, and J. M. Brown. 1993 Complementation of the radiosensitive phenotype in severe combined immunodeficient mice by human chromosome-8 Cancer Res 53:6011-6016
26. Komatsu, K., T. Ohta, Y. Jinno, N. Niikawa, and Y. Okumura. 1993. Functional complementation in mouse-human radiation hybrids assigns the putative murine scid gene to the pericentric region of human chromosome-8. Hum. Mol. Genet 2:1031-1034.
27. Kurimasa, A., Y. Nagata, M. Shimizu, M. Emi, Y. Nakamaura, and M. Oshimura. 1994. A human gene that restores the DNA repair defect in scid mice is located on 8p11.1-q11.1. Hum. Genet 93:21-26
28. Lee, S. E., C. R. Pulaski, D. M. He, D. M. Benjamin, M. J. Voss, J. Y. Um, and E. A. Hendrickson. 1995. Isolation of mammalian mutants that are X-ray sensitive, impaired in DNA double-strand break repair and defective for V(D)J recombination. Mutat. Res. 336:279-291.
29. Lewis, S. M. 1994. The mechanism of V(D)J joining lessons from molecular, immunological, and comparative analysis. Adv. Immunol. 56:27-150.
30. Lieber, M. R., J. E. Hesse, S. Lewis, G. C. Bosnia, N. Rosenberg, K. Mizuchi, M. J. Bosnia, and M. Gellert. 1988. The defect in murine severe combined immunodeficiency: joining of signal sequences but not coding segment in V(D)J recombination. Cell 55:7-16.
31. Mimori, T., and J. A. Hardin. 1986. Mechanisms of interaction between Ku protein and DNA J. Biol. Chem. 261:10375-10379.
32. Mimori, T., J. A. Hardin, and J. A. Steitz. 1986. Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders J. Biol. Chem 261:2274-2278
33. Mimori, T., Y. Ohosone, N. Hama, A. Suwa, M. Akizuki, M. Homma, A. J. Griffith, and J. A. Hardin. 1990. Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/ p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome. Proc. Natl. Acad. Sci. USA 87:1777-1781.
34. Paillard, S., and F. Strauss, 1993. Site-specific proteolytic cleavage of Ku protein bound to DNA. Proteins Struct. Funct. Genet. 15:330-337.
35. Pergola, F., M. Z. Zdzienicka, and M. R. Lieber. 1993. V(D)J recombination in mammalian cell mutants defective in DNA double strand break repair. Mol. Cell. Biol. 13:3464-3471.
36. Peterson, S. R., A. Kurimasa, M. Oshimura, W. S. Dynan, E. M. Bradbury, and D. J. Chen. 1995. Loss of the catalytic subunit of the DNA dependent protein kinase in DNA double-strand break repair mutant mammalian cells. Proc. Natl. Acad. Sci. USA 92:3171-3174.
37. Rathmell, W. K., and G. Chu. 1994. A DNA end-binding factor involved in double-strand break repair and V(D)J recombination. Mol. Cell. Biol. 14: 4741-4748.
38. Kathmell, W. K., and G. Chu. 1994. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91:7623-7627.
39. Reeves, W. H., and Z. M. Sthoeger. 1989. Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen. J. Biol. Chem. 264:5047-5052.
40. Satoh, M., J. Wang, and W. H. Reeves. 1995. Role of free p70 (Ku) subunit in posttranslational stabilization of newly synthesized p80 during DNA-dependent protein kinase assembly. Eur. J. Cell Biol 66:127-135.
41. Shibuya, T., and K. Morimoto. 1993. A review of genotoxicity of 1-ethyl-1-nitrosourea. Mutat. Res 297:3-38.
42. Siminovitch, L. 1976. On the nature of hereditable variation in cultured somatic cells. Cell 7:1-11.
43. Smider, V., W. K. Rathmell, M. R. Lieber, and G. Chu. 1994 Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266:288-291.
44. Taccioli, G. E., H. L. Cheng, A. J. Vargese, G. Whitmore, and F. W. Alt, 1994. A DNA repair defect in Chinese hamster ovary cells affects V(D)J recombination similarly to the murine scid mutation J. Biol. Chem. 269:7439-7442.
45. Taccioli, G. E., T. M. Gottlieb, T. Blunt, A. Priestly, J. Demengeot, R. Mizuta, A. R. Lehmann, F. W. Alt, S. P. Jackson, and P. A. Jeggo. 1994 Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science 265:1442-1445
46. Taccioli, G. E., G. Rathbun, E. Oltz, T. Stamato, P. A. Jeggo, and F. W. Alt. 1993. Impairment of V(D)J recombination in double-strand break repair mutants Science 260:207-210.
47. Takebe, Y., M. Seiki, J.-I. Fujisawa, P. Hoy, K. Yokota, K.-I. Arai, M. Yoshida, and N. Arai. 1988. SRα promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat Mol Cell. Biol. 8:466-472.
48. Thacker, J., A. N. Ganesh, A. Stretch, D. M. Benjamin, A. J. Zahalski, and E. A. Hendrickson. 1994. Gene mutation and V(D)J recombination in the radiosensitive irs lines. Mutagenesis 9:163-168.
49. Tuteja, N., R. Tuteja, A. Ochem, P. Taneja, N. Huang, A. Simonesits, S. Susic, K. Rahman, L. Marusic, J. Chen, J. Zhang, S. Wang, S. Pongor, and A. Falaschi. 1994. Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen. EMBO J. 13:4991-5001.
50. Yaneva, M., and S. Jhiang. 1991 Expression of the Ku protein during cell proliferation. Biochim. Biophys. Acta 1090:181-187
51. Yaneva, M., J. Wen, A. Ayala, and R. Cook. 1989. cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen. J Biol Chem. 264: 13407-13411.
52. Zdzienicka, M. Z. 1995. Mammalian mutants defective in the response to ionizing radiation-induced DNA damage. Mutat. Res. 336:203-213.
53. Zdzienicka, M. Z., W. Jongmans, M. Oshimura, A. Priestly, G. F. Whitmore. and P. A. Jeggo. 1995. Complementation analysis of the murine scid cell line Radiat. Res 143:238-244.
54. Zdzienicka, M. Z., Q. Tran, G. P. van der Schans, and J. W. I. M. Simons. 1988 Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat. Res. 194:239-249.
55. Zdzienicka, M. Z., N. van Wessel, and G. P. van der Schans. 1992. A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair. Radiat. Res. 131: 309-314