Cell cycle block and apoptosis induction in a human melanoma cell line following treatment with 2-methoxyoestradiol: Therapeutic implications?

Melanoma Research

Volumn 13, Issue 2, 2003, Pages 119-127

  Ghosh, Rita ^a   Ott, Ann M ^a   Seetharam, Divya ^a   Slaga, Thomas J ^a   Kumar, Addanki P ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

2 methoxyoestradiol; Apoptosis; Cell cycle; Melanoma; Proliferation

Indexed keywords

2 METHOXYESTRADIOL; DNA FRAGMENT; PROTEIN BAX; PROTEIN P53; REGULATOR PROTEIN;

APOPTOSIS; ARTICLE; CANCER INHIBITION; CELL CYCLE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MELANOMA CELL; MICROSCOPY; NICK END LABELING; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; WESTERN BLOTTING;

APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; BLOTTING, WESTERN; CELL CYCLE; CELL DIVISION; CELL LINE, TUMOR; CELLS, CULTURED; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLINS; DNA FRAGMENTATION; ESTRADIOL; FIBROBLASTS; FLOW CYTOMETRY; G2 PHASE; HUMANS; IN SITU NICK-END LABELING; INTERCALATING AGENTS; MELANOMA; MITOSIS; MODELS, CHEMICAL; PHOSPHORYLATION; PROPIDIUM; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; TIME FACTORS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0013449549     PISSN: 09608931     EISSN: None     Source Type: Journal    
DOI: 10.1097/00008390-200304000-00003     Document Type: Article

References (35)

1. Black J. Malignant melanoma: an update on treatments. Plast Surg Nurs 1999; 19:143-147.
2. White LP. Studies on melanoma: II. Sex and survival in human melanoma. N Engl J Med 1959; 260:789-797.
3. McCarty KS, Wortman J, Stowers S, Lubahn DB, McCarty KS Sr, Seigler HF. Sex steroid receptor analysis in human melanoma. Cancer 1980; 46:1463-1470.
4. Martucci CP, Fishman J. P450 enzymes of estrogen metabolism. Pharmacol Ther 1993; 57:237-257.
5. Zhu BT, Conney AH. Functional role of estrogen metabolism in target cells: review and perspectives. Carcinogenesis 1998; 19:1-27.
6. Seegers JC, Aveiling M-L, van Aswegen CG, Cross M, Koch F, Joubert WS. The cytotoxic effects of estradiol-17 beta, catecholestradiols and methoxyestradiols on dividing MCF-7 and HeLa cells. J Steroid Biochem 1989; 32:797-809.
7. Lottering M-L, Haag M, Seegers JC. Effects of 17 beta-estradiol metabolites on cell cycle events in MCF-7 cells. Cancer Res 1992; 52:5926-5932.
8. D'Amato RJ, Lin CM, Flynn E, Folkman J, Hamel E. 2-Methoxyestradiol, an endogenous mammalian metabolite, inhibits tubulin polymerization by interacting at the colchicines site. Proc Natl Acad Sci USA 1994; 91:3964-3968.
9. Mukhopadhay T, Roth JA. Induction of apoptosis in human lung cancer cells after wild-type p53 activation by methoxyestradiol. Oncogene 1997; 14:379-384.
10. Schumacher G, Kataoka M, Roth JA, Mukhopadhay T. Potent antitumor activity of 2-methoxyestradiol in human pancreatic cancer cell lines. Clin Cancer Res 1999; 5:493-499.
11. Kumar AP, Garcia G, Slaga TJ. 2-Methoxyestradiol blocks cell-cycle progression at G(2)/M phase and inhibits growth of human prostate cancer cells. Mol Carcinog 2001; 31:111-124.
12. Fotsis T, Zhang Y, Petter MS, Adlercreutz H, Montesano R, Nawroth PP, et al. The endogenous oestrogen metabolite 2-methoxyoestradiol inhibits angiogenesis and suppresses tumour growth. Nature 1994; 368:237-239.
13. Krishan A. Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 1975; 66:188-193.
14. Evidokiou A, Ragaatt LJ, Atkins GJ, Findlay DM Calcitonin receptor-mediated growth suppression of HEK-293 cells is accompanied by induction of p21WAF1/CIP1 and G2/M arrest. Mol Endocrinol 1999; 13:1738-1750.
15. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248-254.
16. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680-685.
17. Compton MM. A biochemical hallmark of apoptosis: internucleosomal degradation of the genome. Cancer Metastasis Rev 1992; 11:105-119.
18. Nurse P. Universal control mechanism regulating onset of M-phase. Nature 1990; 344:503-508.
19. Watanbee N, Broome M, Hunter T. Regulation of the human WEE1 HuCDK tyrosinase 15-kinase during the cell cycle. EMBO J 1995; 14:1878-1891.
20. Galaktinov K, Jessus C, Beach D. Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation. Genes Dev 1995; 9:1046-1058.
21. McGowan CH, Russell P. Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15. EMBO J 1993; 12:75-85.
22. Booher RN, Holman PS, Fattaey A. Human Myt1 is a cell cycle-regulated kinase that inhibits Cdc2 but not Cdk2 activity. J Biol Chem 1997; 272:22300-22306.
23. Liu F, Stanton JJ, Wu Z, Piwnica-Worms H. The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex. Mol Cell Biol 1997; 17:571-583.
24. Parker LL, Piwnica-Worms H. Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase. Science 1992; 257:1955-1957.
25. Sanchez Y, Wong C, Thoma RS, Richman R, Wu Z, Piwnica-Worms H, et al. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 1997; 277:1497-1501.
26. Chaturvedi P, Eng WK, Zhu Y, Mattern MR, Mishra R, Hurle MR, et al. Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway. Oncogene 1999; 18:4047-4054.
27. Furnari B, Rhind N, Russell P. Cdc25 mitotic inducer targeted by chk1 DNA damage checkpoint kinase. Science 1997; 277:1495-1497.
28. Pribluda VS, Gubish ER Jr, LaVallee TM, Treston A, Swartz GM, Green SJ. 2-Methoxyestradiol: an endogenous antiangiogenic and antiproliferatire drug candidate. Cancer Metastasis Rev 2000; 19:173-179.
29. Schumacher G, Neuhaus P. The physiological estrogen metabolite 2-methoxyestradiol reduces tumor growth and induces apoptosis in human solid tumors. J Cancer Res Clin Oncol 2001; 127:405-410.
30. Seegers JC, Lottering MC, Grobler CJ, van Papendorp DH, Habbersett RC, Shou Y, et al. The mammalian metabolite, 2-methoxyestradiol, affects P53 levels and apoptosis induction in transformed cells but not in normal cells. J Steroid Biochem Mol Biol 1997; 62:253-267.
31. Volkenandt M, Schlegel U, Nanus DM, Albino AP. Mutational analysis of the human p53 gene in malignant melanoma. Pigment Cell Res 1991; 4:35-40.
32. Albino AP, Vidal MJ, McNutt NS, Shea CR, Prieto VG, Nanus DM, et al. Mutation and expression of the p53 gene in human malignant melanoma. Melanoma Res 1994; 4:35-45.
33. Montano X, Shamsher M, Whitehead P, Dawson K, Newton J. Analysis of p53 in human cutaneous melanoma cell lines. Oncogene 1994; 9:1455-1459.
34. Satyamoorty K, Chehab NH, Watterman MJF, Lien MC, El-Diery WS, Herlyn M, et al. Aberrant regulation and function of wild-type p53 in radioresistant melanoma cells. Cell Growth Differ 2000; 11:467-474.
35. DelPrete SA, Maurer LH, O'Donnell J. Combination chemotherapy with cisplatin, carmustine, dacarbazine, and tamoxifen in metastatic melanoma. Cancer Treat Rep 1984; 68:1403-1405.