Efficient purification and kinetic characterization of a bimodular derivative of the erythromycin polyketide synthase

European Journal of Biochemistry

Volumn 267, Issue 2, 2000, Pages 520-526

  Bycroft, Matthew ^a   Weissman, Kira J ^a   Staunton, James ^a   Leadlay, Peter F ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

6 deoxyerythronolide B synthase 1 thioesterase (DEBS 1 TE); Kinetic characterization; Polyketide synthase; Purification

Indexed keywords

AMMONIUM SULFATE; CARRIER PROTEIN; ERYTHROMYCIN DERIVATIVE; POLYKETIDE SYNTHASE;

ARTICLE; CATALYSIS; CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; REACTION ANALYSIS; SACCHAROPOLYSPORA; STEADY STATE;

ACYL COENZYME A; KINETICS; MULTIENZYME COMPLEXES; PYRONES; SACCHAROPOLYSPORA;

ACTINOBACTERIA (CLASS); FELIS CATUS; ILEX COOKII; SACCHAROPOLYSPORA; SACCHAROPOLYSPORA ERYTHRAEA; UNCULTURED ACTINOMYCETE;

EID: 0001059115     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01025.x     Document Type: Article

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