Study of the Ecology of Fresh Sausages and Characterization of Populations of Lactic Acid Bacteria by Molecular Methods

Applied and Environmental Microbiology

Volumn 70, Issue 4, 2004, Pages 1883-1894

  Cocolin, Luca ^a   Rantsiou, Kalliopi ^a   Iacumin, Lucilla ^a   Urso, Rosalinda ^a   Cantoni, Carlo ^b   Comi, Giuseppe ^a  

^a UNIVERSITY OF UDINE   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; DNA; ELECTROPHORESIS; FOOD STORAGE; ORGANIC ACIDS; RNA; YEAST;

DENATURING GRADIENT GEL ELECTROPHORESIS (DGEE); LACTIC ACID BACTERIA (LAB); SAUSAGES;

ECOLOGY;

REPETITIVE DNA;

MICROBIOLOGY;

ANALYTIC METHOD; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BROCHOTHRIX THERMOSPHACTA; CAPRONIA MANSONII; CONTROLLED STUDY; DEBARYOMYCES HANSENII; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA DETERMINATION; DNA EXTRACTION; ECOLOGY; ENTEROCOCCUS CASSELIFLAVUS; FOOD CONTAMINATION; FOOD STORAGE; FUNGUS ISOLATION; LACTIC ACID BACTERIUM; LACTOBACILLUS CASEI; LACTOBACILLUS CURVATUS; LACTOBACILLUS SAKEI; LACTOCOCCUS LACTIS; LEUCONOSTOC; MONITOR; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; POPULATION; RANDOM AMPLIFIED POLYMORPHIC DNA; RNA ANALYSIS; RNA EXTRACTION; STAPHYLOCOCCUS; STAPHYLOCOCCUS XYLOSUS; STORAGE TEMPERATURE; YEAST;

ANIMALS; BASE SEQUENCE; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; ECOSYSTEM; FOOD MICROBIOLOGY; HYDROGEN-ION CONCENTRATION; LACTOBACILLUS; MEAT PRODUCTS; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); BROCHOTHRIX THERMOSPHACTA; CAPRONIA; CAPRONIA MANSONII; DEBARYOMYCES; DEBARYOMYCES HANSENII; ENTEROCOCCUS; ENTEROCOCCUS CASSELIFLAVUS; FUNGI; LACTOBACILLUS; LACTOBACILLUS CASEI; LACTOBACILLUS CURVATUS; LACTOBACILLUS SAKEI; LACTOCOCCUS; LACTOCOCCUS LACTIS; LACTOCOCCUS LACTIS SUBSP. LACTIS; LEUCONOSTOC; LEUCONOSTOC MESENTEROIDES; POSIBACTERIA; STAPHYLOCOCCUS; STAPHYLOCOCCUS PHAGE 3A; STAPHYLOCOCCUS SP.; STAPHYLOCOCCUS XYLOSUS; STREPTOCOCCUS; UNCULTURED BACTERIUM; UNCULTURED STAPHYLOCOCCUS SP.;

EID: 9744228256     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.4.1883-1894.2004     Document Type: Article

References (42)

1. Altschul, S. F., T. L. Madden, A. A. Shaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Anderson, L. 1997. Bioprotective cultures for fresh sausages. Fleischwirtschaft 77:635-637.
3. Andrigetto, C., L. Zampese, and A. Lombardi. 2001. RAPD-PCR characterization of lactobacilli isolated from artisanal meat plants and traditional fermented sausages of Veneto region (Italy). Lett. Appl. Microbiol. 33:26-30.
4. ben Omar, N., and F. Ampe. 2000. Microbial community dynamics during production of the Mexican fermented maize dough pozol. Appl. Environ. Microbiol. 66:3664-3673.
5. Berthier, F., and S. D. Ehrlich. 1999. Genetic diversity within Lactobacillus sakei and Lactobacillus curvatus and design of PCR primers for its detection using randomly amplified polymorphic DNA. Int. J. Syst. Bacteriol. 49:997-1007.
6. Boles, J. A., V. L. Mikkelsen, and J. E. Swan. 1998. Effects of chopping time, meat source and storage temperatures on the colour of New Zealand type fresh beef sausages. Meat Sci. 49:79-88.
7. Bouton, Y., P. Guyot, E. Beuvier, P. Tailliez, and R. Grappin. 2002. Use of PCR-based methods and PFGE for typing and monitoring homofermentative lactobacilli during Comté cheese ripening. Int. J. Food Microbiol. 76: 27-38.
8. Casalinuovo, F., A. Cacia, A. Scognamiglio, and L. Bontempo. 2001. Microbiological aspects and main causes of contamination of some typical meat products. Ind. Aliment. XL:159-162.
9. Cocolin, L., L. F. Bisson, and D. A. Mills. 2000. Direct profiling of the yeast dynamics in wine fermentations. FEMS Microbiol. Lett. 189:81-87.
10. Cocolin, L., M. Manzano, C. Cantoni, and G. Comi. 2001. Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages. Appl. Environ. Microbiol. 67:5113-5121.
11. Cocolin, L., K. Rantsiou, L. Iacumin, C. Cantoni, and G. Comi. 2002. Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods. Appl. Environ. Microbiol. 68:6273-6282.
12. Coppola, S., G. Blaiotta, D. Ercolini, and G. Moschetti. 2001. Molecular evaluation of microbial diversity occuring in different types of Mozzarella cheese. J. Appl. Microbiol. 90:414-420.
13. Ferris, S. Y., G. Muyzer, and D. M. Ward. 1996. Denaturing gradient gel electrophoresis profiles of 16S rRNA defined populations inhabiting hot spring microbial mat community. Appl. Environ. Microbiol. 62:340-346.
14. Gevers, D., G. Huys, and J. Swings. 2001. Applicability of rep-PCR finger-printing for identification of Lactobacillus species. FEMS Microbiol. Lett. 205:31-36.
15. Godon, J. J., E. Zumstein, P. Dabert, F. Habouzit, and R. Moletta. 1997. Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis. Appl. Environ. Microbiol. 63:2802-2813.
16. Head, I. M., J. R. Saunders, and R. W. Pickup. 1998. Microbial evolution, diversity and ecology: a decade of ribosomal RNA analysis of uncultivated microorganisms. Microb. Ecol. 35:1-21.
17. Heilig, H. G. H. J., E. G. Zoetendal, E. E. Vaughand, P. Marteau, A. D. L. Akkermans, and W. M. de Vos. 2002. Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl. Environ. Microbiol. 68:114-123.
18. Hugenholtz, P., B. M. Goebel, and N. R. Pace. 1998. Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J. Bacteriol. 180:4765-4774.
19. Hugenholtz, P., and N. R. Pace. 1996. Identifying microbial diversity in the natural environment: a molecular phylogenetic approach. Trends Biotechnol. 14:90-97.
20. Klijn, N., A. H. Weerkamp, and W. M. deVos. 1991. Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes. Appl. Environ. Microbiol. 57:3390-3393.
21. Meroth, C. B., J. Walter, C. Hertel, J. Brandt, and W. P. Hammes. 2003. Monitoring the bacterial population dynamics in sourdough fermentation process by using PCR-denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 69:475-482.
22. Meunier, J. R., and P. A. D. Grimont. 1993. Factors affecting reproducibility of random amplified polymorphic DNA-fingerprinting. Res. Microbiol. 144:373-379.
23. Mills, D. A., E. A. Johannsen, and L. Cocolin. 2002. Yeast diversity and persistance in botrytis-affected wine fermentation. Appl. Environ. Microbiol. 68:4884-4893.
24. Muyzer, G. 1999. DGGE/TGGE a method for identifying genes from natural ecosystems. Curr. Opin. Microbiol. 2:317-322.
25. Muyzer, G., E. D. De Waal, and A. G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59:695-700.
26. Muyzer, G., and K. Smalla. 1998. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Leeuwenhoek 73:127-141.
27. Myers, R. M., T. Maniatis, and L. S. Lerman. 1987. Detection and localization of single base change by denaturing gel electrophoresis. Methods Enzymol. 155:501-527.
28. Olive, D. M., and P. Bean. 1999. Principles and application of methods for DNA based typing of microbial organisms. J. Clin. Microbiol. 37:1661-1669.
29. Pace, N. R. 1997. A molecular view of microbial diversity and the biosphere. Science 276:734-740.
30. Pezzotti, G., R. Mioni, M. Grimaldi, and A. Ponzoni. 2001. Valutazione microbiologica di carni macinate e preparazioni di carne. Ingegneria Aliment. 16:18-26.
31. Randazzo, C. L., S. Torriani, D. L. Akkermans, W. M. deVos, and E. E. Vaughan. 2002. Diversity, dynamics, and activity of bacterial communities during production of an artisanal Sicilian cheese as evaluated by 16S rRNA analysis. Appl. Environ. Microbiol, 68:1882-1892.
32. Roller, S., S. Sagoo, R. Board, T. O'Mahony, E. Caplice, G. Fitzgerald, M. Fogden, M. Owen, and H. Fletcher. 2002. Novel combination of chitosan, carnocin and sulphite for the preservation of chilled pork sausages. Meat Sci. 62:165-177.
33. Santos, N. N., R. C. Santos-Mendosa, Y. Sanz, T. Bolumar, M.-C. Aristoy, and F. Toldrà. 2001. Hydrolysis of pork muscle sarcoplasmatic proteins by Debaryomyces hansenii. Int. J. Food Microbiol. 68:199-206.
34. Sheffield, V. C., D. R. Cox, L. S. Lerman, and R. M. Myers. 1989. Attachment of a 40-base pairs G+C rich sequence (GC clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc. Natl. Acad. Sci. USA 86:297-303.
35. van Beek, S., and F. G. Priest. 2002. Evolution of the lactic acid bacterial community during malt whisky fermentation: a polyphasic study. Appl. Environ. Microbiol. 68:297-305.
36. Vauterin, L., and P. Vauterin. 1992. Computer-aided objective comparison of electrophoretic patterns for grouping and identification of microorganisms. Eur. Microbiol. 1:37-41.
37. Versalovic, J., M. Schneider, F. J. De Bruijn, and J. R. Lupski. 1994. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol. Cell. Biol. 5:25-40.
38. Walter, J., C. Hertel, G. W. Tannock, C. M. Lis, K. Munro, and W. P. Hammes. 2001. Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 67:2578-2585.
39. Ward, D. M., M. J. Ferris, S. C. Nold, and M. M. Bateson. 1998. A natural view of microbial biodiversity within hot spring cyanobacterial mat communities. Microbiol. Mol. Biol. Rev. 62:1353-1367.
40. Zambonelli, C., F. Papa, P. Romano, G. Suzzi, and L. Grazia. 1992. Microbiologia dei salumi. Edagricole, Bologna, Italy.
41. Zanardi, E., E. Novelli, G. P. Ghiretti, V. Dorigoni, and R. Chizzolini. 1999. Colour stability and vitamin E content of fresh and processed pork. Food Chem. 67:163-171.
42. Zoetendal, E. G., A. D. L. Akkermans, and W. M. de Vos. 1998. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl. Environ. Microbiol. 64:3854-3859.