Staphylococcus aureus DNA ligase: Characterization of its kinetics of catalysis and development of a high-throughput screening compatible chemiluminescent hybridization protection assay

Biochemical Journal

Volumn 383, Issue 3, 2004, Pages 551-559

  Gul, Sheraz ^a   Brown, Richard ^b   May, Earl ^c   Mazzulla, Marie ^c   Smyth, Martin G ^a   Berry, Colin ^d   Morby, Andrew ^d   Powell, David J ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

^b Molec Light Technol Res Limited   (United Kingdom)

^c GLAXOSMITHKLINE   (United States)

^d CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

Acridinium ester label; Chemiluminescent hybridization protection assay; High throughput screening; Kinetic characterization; Lead discovery; Staphylococcus aureus DNA ligase

Indexed keywords

CATALYSIS; CHEMILUMINESCENCE; ENZYME INHIBITION; ENZYME KINETICS; ESCHERICHIA COLI; ESTERS; IONIZATION; RADIOMETRY; THROUGHPUT;

CHLOROQUINE DIPHOSPHATE; EUKARYOTIC ENZYMES; FREE ENZYMES; LIGASES;

DNA;

ACRIDINE DERIVATIVE; ACRIDINIUM ESTER; CHLOROQUINE; DNA; DNA LIGASE INHIBITOR; DOXORUBICIN; ENZYME INHIBITOR; ESTER; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CATALYSIS; CHEMILUMINESCENT HYBRIDIZATION PROTECTION ASSAY; ENZYME ASSAY; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; NONHUMAN; PH; PRIORITY JOURNAL; RADIOMETRY; STAPHYLOCOCCUS AUREUS;

ACRIDINES; CATALYSIS; CHEMILUMINESCENT MEASUREMENTS; CLONING, MOLECULAR; DNA LIGASES; DNA, BACTERIAL; HYDROLYSIS; KINETICS; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDES; RESEARCH DESIGN; STAINING AND LABELING; STAPHYLOCOCCUS AUREUS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; FELIS CATUS; PROKARYOTA; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 9144226894     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040054     Document Type: Article

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