Improvement in hydrolytic antibody activity by change in haptenic structure from phosphate to phosphonate with retention of a common leaving-group determinant: Evidence for the 'flexibility' hypothesis

Biochemical Journal

Volumn 376, Issue 3, 2003, Pages 813-821

  Gul, Sheraz ^a,d   Sonkaria, Sanjiv ^a   Pinitglang, Surapong ^a,e   Florez Alvarez, José ^a   Hussain, Syeed ^a   Thomas, Emrys W ^b   Ostler, Elizabeth L ^c   Gallacher, Gerard ^c   Resmini, Marina ^a   Brocklehurst, Keith ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF SALFORD   (United Kingdom)

^c UNIVERSITY OF BRIGHTON   (United Kingdom)

^d GLAXOSMITHKLINE   (United Kingdom)

^e UNIVERSITY OF THE THAI CHAMBER OF COMMERCE   (Thailand)

Author keywords

Flexibility of hapten; Hydrolytic catalytic antibody; Kinetic characterization; Leaving group determinant; Phosphonate synthesis

Indexed keywords

ANTIBODIES; BIOLOGICAL ORGANS; CATALYST ACTIVITY; ESTERS; IMMUNOLOGY;

IMMUNOGENS;

BIOCHEMISTRY;

ANTIGEN; CARBONIC ACID; CATALYTIC ANTIBODY; ESTER; HAPTEN; HYDROLASE; PHOSPHATE; PHOSPHONIC ACID DERIVATIVE;

ANTIBODY PRODUCTION; ARTICLE; BINDING AFFINITY; CATALYSIS; PH; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN STRUCTURE; PROTEIN SYNTHESIS;

ANTIBODIES, CATALYTIC; HAPTENS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MODELS, CHEMICAL; MOTION; PHOSPHATES; PHOSPHONIC ACIDS;

EID: 9144238827     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030716     Document Type: Article

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