Mass spider silk production through targeted gene replacement in Bombyx mori

Proceedings of the National Academy of Sciences of the United States of America

Volumn 115, Issue 35, 2018, Pages 8757-8762

  Xu, Jun ^a   Dong, Qinglin ^b   Yu, Ye ^a   Niu, Baolong ^c   Ji, Dongfeng ^c   Li, Muwang ^d   Huang, Yongping ^a   Chen, Xin ^b   Tan, Anjiang ^a  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^b FUDAN UNIVERSITY   (China)

^c INSTITUTE OF PLANT PROTECTION AND MICROBIOLOGY   (China)

^d JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Bombyx mori; Genome editing; Spider silk; TALEN

Indexed keywords

MASP1 PROTEIN; SILK FIBROIN; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG; FIBROIN; SPIDROIN 1;

ARTICLE; CHIMERA; COCOON; CONTROLLED STUDY; FIBH GENE; GENE; GENE EXPRESSION; GENE INSERTION; GENE SEQUENCE; GENE TARGETING; GENOME; MASP1 GENE; MOLECULAR MECHANICS; NEPHILA CLAVIPES; NONHUMAN; PRIORITY JOURNAL; SERICULTURE; SPIDER; TRANSGENIC ORGANISM; TRANSPOSON; ANIMAL; BIOSYNTHESIS; BOMBYX; GENETIC ENGINEERING; GENETICS; METABOLISM; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BOMBYX; FIBROINS; GENETIC ENGINEERING; SPIDERS;

EID: 85053627577     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1806805115     Document Type: Article

References (35)

1. Tao H, Kaplan DL, Omenetto FG (2012) Silk materials—A road to sustainable high technology. Adv Mater 24:2824–2837.
2. Tokareva O, Jacobsen M, Buehler M, Wong J, Kaplan DL (2014) Structure-function-property-design interplay in biopolymers: Spider silk. Acta Biomater 10:1612–1626.
3. Blamires SJ, Blackledge TA, Tso IM (2017) Physicochemical property variation in spider silk: Ecology, evolution, and synthetic production. Annu Rev Entomol 62:443–460.
4. Scheibel T (2004) Spider silks: Recombinant synthesis, assembly, spinning, and engineering of synthetic proteins. Microb Cell Fact 3:14.
5. Yip EC, Rayor LS (2014) Maternal care and subsocial behaviour in spiders. Biol Rev Camb Philos Soc 89:427–449.
6. Andersson M, et al. (2017) Biomimetic spinning of artificial spider silk from a chimeric minispidroin. Nat Chem Biol 13:262–264.
7. Jansson R, et al. (2016) Functionalized silk assembled from a recombinant spider silk fusion protein (Z-4RepCT) produced in the methylotrophic yeast Pichia pastoris. Biotechnol J 11:687–699.
8. Lazaris A, et al. (2002) Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. Science 295:472–476.
9. Zhang Y, et al. (2008) Expression of EGFP-spider dragline silk fusion protein in BmN cells and larvae of silkworm showed the solubility is primary limit for dragline proteins yield. Mol Biol Rep 35:329–335.
10. Hauptmann V, et al. (2013) Native-sized spider silk proteins synthesized in planta via intein-based multimerization. Transgenic Res 22:369–377.
11. Teulé F, et al. (2012) Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties. Proc Natl Acad Sci USA 109:923–928.
12. Goldsmith MR, Shimada T, Abe H (2005) The genetics and genomics of the silkworm, Bombyx mori. Annu Rev Entomol 50:71–100.
13. Mondal M, Trivedy K, Kumar SN (2007) The silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn.—A review. Caspian J Environ Sci 5:63–76.
14. Chevillard M, Couble P, Prudhomme JC (1986) Complete nucleotide sequence of the gene encoding the Bombyx mori silk protein P25 and predicted amino acid sequence of the protein. Nucleic Acids Res 14:6341–6342.
15. Yamaguchi K, et al. (1989) Primary structure of the silk fibroin light chain determined by cDNA sequencing and peptide analysis. J Mol Biol 210:127–139.
16. Zhou CZ, et al. (2000) Fine organization of Bombyx mori fibroin heavy chain gene. Nucleic Acids Res 28:2413–2419.
17. Tanaka K, et al. (1999) Determination of the site of disulfide linkage between heavy and light chains of silk fibroin produced by Bombyx mori. Biochim Biophys Acta 1432: 92–103.
18. Kuwana Y, Sezutsu H, Nakajima K, Tamada Y, Kojima K (2014) High-toughness silk produced by a transgenic silkworm expressing spider (Araneus ventricosus) dragline silk protein. PLoS One 9:e105325.
19. Takasu Y, et al. (2010) Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection. Insect Biochem Mol Biol 40:759–765.
20. Ma S, et al. (2012) Highly efficient and specific genome editing in silkworm using custom TALENs. PLoS One 7:e45035.
21. Sajwan S, et al. (2013) Efficient disruption of endogenous Bombyx gene by TAL effector nucleases. Insect Biochem Mol Biol 43:17–23.
22. Wang Y, et al. (2013) The CRISPR/Cas system mediates efficient genome engineering in Bombyx mori. Cell Res 23:1414–1416.
23. Gaj T, Gersbach CA, Barbas CF, 3rd (2013) ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol 31:397–405.
24. Wang Y, et al. (2014) Site-specific, TALENs-mediated transformation of Bombyx mori. Insect Biochem Mol Biol 55:26–30.
25. He YX, et al. (2012) N-Terminal domain of Bombyx mori fibroin mediates the assembly of silk in response to pH decrease. J Mol Biol 418:197–207.
26. Shimizu K, et al. (2007) Structure and function of 5′-flanking regions of Bombyx mori fibroin heavy chain gene: Identification of a novel transcription enhancing element with a homeodomain protein-binding motif. Insect Biochem Mol Biol 37:713–725.
27. Wang SP, Guo TQ, Guo XY, Huang JT, Lu CD (2006) Structural analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori. Acta Biochim Biophys Sin (Shanghai) 38:507–513.
28. Wang SP, Guo TQ, Guo XY, Huang JT, Lu CD (2006) In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector. Biochem Biophys Res Commun 341:1203–1210.
29. Huang P, et al. (2011) Heritable gene targeting in zebrafish using customized TALENs. Nat Biotechnol 29:699–700.
30. Tan A, et al. (2013) Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori. Proc Natl Acad Sci USA 110:6766–6770.
31. Oyama F, Mizuno S, Shimura K (1984) Predominant synthesis of fibroin heavy and light chains on the membrane-bound polysomes prepared from the posterior silk gland of the silkworm, Bombyx mori. J Biochem 96:1143–1153.
32. Ma S, et al. (2014) Genome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor. Sci Rep 4:6867.
33. Cui Y, et al. (2018) New insight into the mechanism underlying the silk gland biological process by knocking out fibroin heavy chain in the silkworm. BMC Genomics 19:215.
34. Tamura T, et al. (2000) Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector. Nat Biotechnol 18:81–84, and erratum (2000) 18:559.
35. Fraser MJ, Jr (2012) Insect transgenesis: Current applications and future prospects. Annu Rev Entomol 57:267–289.