Expression of EGFP-spider dragline silk fusion protein in BmN cells and larvae of silkworm showed the solubility is primary limit for dragline proteins yield

Molecular Biology Reports

Volumn 35, Issue 3, 2008, Pages 329-335

  Zhang, Yuansong ^a,b   Hu, Junhua ^b   Miao, Yungen ^c   Zhao, Aichun ^b   Zhao, Tianfu ^a   Wu, Dayang ^b   Liang, Liefeng ^b   Miikura, Ayumi ^a   Shiomi, Kunihiro ^a   Kajiura, Zenta ^a   Nakagaki, Masao ^a  

^a SHINSHU UNIVERSITY   (Japan)

^b SOUTHWEST UNIVERSITY   (China)

^c ZHEJIANG UNIVERSITY   (China)

Author keywords

Bac to Bac BmNPV Baculovirus expression system; BmN cell; Fusion protein; Silkworm larvae; Solubility; Spider dragline silk

Indexed keywords

EGFP SPIDER DRAGLINE SILK FUSION PROTEIN; HYBRID PROTEIN;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE; IMMUNOBLOTTING; LARVA; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN AGGREGATION; PROTEIN EXPRESSION; SILKWORM; SOLUBILITY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BOMBYX; CELL LINE; DNA, COMPLEMENTARY; FIBROINS; GENE EXPRESSION; GENES, REPORTER; LARVA; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SPIDERS;

ARANEAE; BOMBYX MORI; NEPHILA CLAVATA;

EID: 48249103129     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-007-9090-6     Document Type: Article

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