Cloning, recombinant expression and characterization of a new phytase from Penicillium chrysogenum

Microbiological Research

Volumn 170, Issue , 2015, Pages 205-212

  Ribeiro Corrêa, Thamy Lívia ^a   de Queiroz, Marisa Vieira ^a   de Araújo, Elza Fernandes ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

Author keywords

Characterization; Molecular cloning; Penicillium chrysogenum; Penicillium griseoroseum PG63; Phytase

Indexed keywords

CLONE CELLS; CLONING; DIGITAL STORAGE; GENES; PHYSICAL LAYER; RECOMBINANT PROTEINS;

EXPRESSION OF HETEROLOGOUS PROTEINS; ISOELECTRIC POINT (PI); MOLECULAR CLONING; PENICILLIUM CHRYSOGENUM; PENICILLIUM GRISEOROSEUM PG63; PHYTASES; RECOMBINANT EXPRESSION; RECOMBINANT STRAINS;

ENZYMES;

ANIMALIA; FUNGI; GLYCINE MAX; PENICILLIUM CHRYSOGENUM; PENICILLIUM GRISEOROSEUM;

PHYTASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; CHEMISTRY; ENZYME ACTIVATION; GENE DOSAGE; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PENICILLIUM CHRYSOGENUM; SEQUENCE ALIGNMENT; THERMODYNAMICS;

6-PHYTASE; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME ACTIVATION; GENE DOSAGE; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PENICILLIUM CHRYSOGENUM; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; THERMODYNAMICS;

EID: 85050292845     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2014.06.005     Document Type: Article

References (52)

1. Anasontzis G.E., Zerva A., Stathopoulou P.M., Haralampidis K., Diallinas G., Karagouni A.D., et al. Homologous overexpression of xylanase in Fusarium oxysporum increases ethanol productivity during consolidated bioprocessing (CBP) of lignocellulosics. J Biotechnol 2011, 152:16-23.
2. Berka R.M., Rey M.W., Brown K.M., Byun T., Klotz A.V. Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus. Appl Environ Microbiol 1998, 64:4423-4427.
3. Bohn L., Meyer A.S., Rasmussen S.K. Phytate: impact on environment and human nutrition. A challenge for molecular breeding. J Zheijang Univ 2008, 9:165-191.
4. Boyce A., Walsh G. Purification and characterisation of an acid phosphatase with phytase activity from Mucor hiemalis Wehmer. J Biotechnol 2007, 132:82-87.
5. Bradford M.M. Rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
6. Cardoso P.G., Ribeiro J.B., Teixeira J.A., Queiroz M.V., Araújo E.F. Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum. J Ind Microbiol Biotechnol 2008, 35:159-166.
7. Cho J.S., Lee C.W., Kang S.H., Lee J.C., Bok J.D., Moon Y.S., et al. Purification and characterization of a phytase from Pseudomonas syringae MOK1. Curr Microbiol 2003, 47:290-294.
8. Coban H.B., Demirci A. Screening of phytase producers and optimization of culture conditions for submerged fermentation. Bioprocess Biosyst Eng 2014, 37:609-616.
9. Corrêa T.L.R., Zubieta M.P., Teixeira J.A., Queiroz M.V., Araújo E.F. Carboxyl ester hydrolase from Penicillium expansum: cloning, characterization and over production by Penicillium griseoroseum. J Appl Microbiol 2013, 115:114-124.
10. Dias E.S., Araújo E.F., Guimarães V.W., Coelho J.L., Silva D.O. Productionand regeneration of Penicillium expansum and Penicillium griseoroseum protoplasts. Rev Microbiol 1997, 28:116-120.
11. Fugthong A., Boonyapakron K., Sornlek W., Tanapongpipat S., Eurwilaichitr L., Pootanakit K. Biochemical characterization and in vitro digestibility assay of Eupenicillium parvum (BCC17694) phytase expressed in Pichia pastoris. Protein Expr Purif 2010, 70:60-67.
12. Gargova S., Sariyska M., Angelov A., Stoilova I. Aspergillus niger pH 2.1 optimum acid phosphatase with high affinity for phytase. Folia Microbiol 2006, 51:541-545.
13. Haefner S., Knietsch A., Scholten E., Braun J., Lohscheidt M., Zelder O. Biotechnological production and application of phytases. Appl Microbiol Biotechnol 2005, 68:588-597.
14. Han Y., Wilson D.B., Lei X. Expression of an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae. Appl Environ Microbiol 1999, 65:1915-1918.
15. Heinonen J.K., Lahti R.J. A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase. Anal Biochem 1981, 113:313-317.
16. Inoue H., Nojima H., Okayama H. High efficiency transformation of Escherichia coli with plasmids. Gene 1990, 96:23-28.
17. Kostrewa D., Wyss M., D'arcy A., van Loon A.P.G.M. Crystal structure of Aspergillus niger pH 2.5 optimum acid phosphatase at 2.4A resolution. J Mol Biol 1997, 288:965-974.
18. Laskowsky R.A., Watson J.D., Thornton J.M. ProFunc: a server for predicting protein function from 3D structure. Nucleic Acids Res 2005, 33:W89-W93.
19. Lee J., Choi Y., Lee P.-C., Kang S., Bok J., Cho J. Recombinant production of Penicillium oxalicum PJ3 phytase in Pichia pastoris. World J Microbiol Biotechnol 2007, 23:336-443.
20. Liu H., Bao X. Overexpression of the chitosanase gene in Fusarium solani via Agrobacterium tumefaciens-mediated transformation. Curr Microbiol 2009, 58:279-282.
21. Lopes F.J.F., Araújo E.F., Queiroz M.V. Easy detection of green fluorescent protein multicopy transformants in Penicillium griseoroseum. Genet Mol Res 2004, 3:449-455.
22. Ma Z.-Y., Pu S.-C., Jiang J.-J., Huang B., Fan M.-Z., Li Z.-Z. A novel thermostable phytase from the fungus Aspergillus aculeatus RCEF 4894: gene cloning and expression in Pichia pastoris. World J Microbiol Biotechnol 2011, 27:679-686.
23. Maller A., Vici A.C., Facchini F.D.A., Silva T.M., Kamimura E.S., Rodrigues M.I., et al. Increase of the phytase production by Aspergillus japonicus and its biocatalyst potential on chicken feed treatment. J Basic Microbiol 2013, 53:1-9.
24. Noorbatcha I.A., Sultan A.M., Salleh H.M., Amid A. Understanding thermostability factors of Aspergillus niger PhyA phytase: a molecular dynamics study. Protein J 2013, 309-316.
25. Oh B.-C., Choi W.-C., Park S., Kim Y.-O., Oh T.-K. Biochemical properties and substrate specificities of alkaline and histidine acid phytases. Appl Microbiol Biotechnol 2004, 63:362-372.
26. Pandee P., Summpunn P., Wiyakrutta S., Isarangkul D., Meevootisom V. A thermostable phytase from Neosartorya spinosa BCC 41923 and its expression in Pichia pastoris. J Microbiol 2011, 49:257-264.
27. Pasamontes L., Haiker M., Wyss M., Tessier M., van Loon A.P. Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus. Appl Environ Microbiol 1997, 63:1696-1700.
28. Pereira J.F., de Queiroz M.V., Lopes F.J., Rocha R.B., Daboussi M.J., Araújo E.F. Characterization, regulation and phylogenetic analyses of the Penicillium griseoroseum nitrate reductase gene and its use as selection marker for homologous transformation. Can J Microbiol 2004, 50:891-900.
29. Piddington C.S., Houston C.S., Paloheimo M., Cantrell M., Miettinen-Oinonen A., Nevalainen J.R. The cloning and sequencing of the genes encoding phytase (phy) and pH 2,5 optimum acid phosphatase (aph) from Aspergillus niger var. awamori. Gene 1993, 133:55-62.
30. Punt P.J., Dingemanse M.A., Jacobs-Maijsing B.J., Pouwels P.H., van den Hondel C.A. Isolation and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Aspergillus nidulans. Gene 1998, 69:49-57.
31. Punt P.J., van Biezen N., Conesa A., Albers A., Mangnus J., van der Hondel C. Filamentous fungi as cell factories for heterologous protein production. Trends Biotechnol 2002, 20:200-206.
32. Queiroz M.V., Barros A.O., Barros E.G., Guimarães W.V., Araújo E.F. Transformation of Penicillium griseoroseum nitrate reductase mutant with the nia gene from Fusarium oxysporum. Can J Microbiol 1998, 44:487-489.
33. Rao D.E.C.S., Rao K.V., Reddy T.P., Reddy V.D. Molecular characterization, physicochemical properties, known and potential applications of phytases: an overview. Crit Rev Biotechnol 2009, 29:182-198.
34. Sambrook J., Russel D.W. Molecular Cloning 2001, vol. 3. New York, Cold Spring Harbor Laboratory Press. 3rd ed.
35. Sapna B., Sing Phytase production by Aspergillus oryzae in solid-state fermentation and its applicability in the dephytinization of wheat bran. Appl Biochem Biotechnol 2014, 10.1007/s12010-014-0974-3.
36. Selle P.H., Ravindran V., Caldwell R.A., Bryden W.L. Phytate and phytase: consequences for protein utilization. Nutr Res Rev 2000, 13:255-278.
37. Southern E. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975, 98:503-517.
38. Specht C.A., Dirusso C.C., Novotny C.P., Ullrich R.C. A method for extracting high molecular weight deoxyribonucleic acid from fungi. Anal Biochem 1982, 119:158-163.
39. Spier M.R., Salmon D.N.X., Binati R.L., Piva L.C., Medeiros A.B.P., Soccol C.R. Stability of new macromycetes phytases under room, cooling and freezing temperatures of storage. World Acad Sci Eng Technol 2012, 67:627-631.
40. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24:1596-1599.
41. Teixeira J.A., Gonçalves D.B., Queiroz M.V., Araújo E.F. Improved pectinase production in Penicillium griseoroseum recombinant strains. J Appl Microbiol 2011, 111:818-825.
42. Teixeira J.A., Ribeiro J.B., Gonçalves D.B., Queiroz M.V., Araújo E.F. Overproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene. Appl Biochem Biotechnol 2013, 169:1965-1977.
43. Teixeira J.A., Corrêa T.L.R., Queiroz M.V., Araújo E.F. pH-dependent effect of pectinase secretion in Penicillium griseoroseum recombinant strains. J Basic Microbiol 2013, 10.1002/jobm.201200325.
44. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22:4673-4680.
45. Ullah A.H.J., Gibson D.M. Extracellular phytase (E.C. 3.1.3.8) from Aspergillus ficuum NRRL 3135: purification and characterization. Prep Biochem 1987, 17:63-71.
46. Van Hartingsveldt W., van Zeijl C.M., Harteveld G.M., Gouka R.J., Suykerbuyk M.E., Luiten R.G., et al. Gene 1993, 127:187-194.
47. Vohra A., Satyanarayana T. Phytases microbial sources, production, purification, and potential biotechnological applications. Crit Rev Biotechnol 2003, 23:29-60.
48. Yang J., Zhao X., Liang L., Xia Z., Lei L., Niu X., et al. Overexpression of a cuticle-degrading protease Ver112 increases the nematicidal activity of Paecilomyces lilacinus. Appl Microbiol Biotechnol 2011, 89:1895-1903.
49. Yi Z., Kornegay E.T. Sites of phytase activity in the gastrointestinal tract of young pigs. Anim Feed Sci Technol 1996, 61:361-368.
50. Wyss M., Pasamontes L., Rémy R., Kohler J., Kusznir E., Gadient M., et al. Comparison of thermostability properties of tree acid phosphatases from molds: Aspergillus fumigatus phytase, A. niger phytase, and A. niger pH 2.5 acid phosphatase. Appl Environ Microbiol 1998, 64:4446-4451.
51. Wyss M., Brugger R., Kronenberger A., Rémy R., Fimbel R., Oesterhelt G., et al. Biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. Appl Environ Microbiol 1999, 65:367-373.
52. Zhao D.-M., Wang M., Mu X.-J., Sun M.-L., Wang X.-Y. Screening, cloning and over expression of Aspergillus niger phytase (phyA) in Pichia pastoris with favourable characteristics. Lett Appl Microbiol 2007, 45:522-528.