Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Nature Biotechnology

Volumn 36, Issue 6, 2018, Pages 505-508

  Bao, Zehua ^a   HamediRad, Mohammad ^a   Xue, Pu ^a   Xiao, Han ^a,e   Tasan, Ipek ^b   Chao, Ran ^a   Liang, Jing ^c   Zhao, Huimin ^a,b,c,d  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c Agency for Science   (Singapore)

^d University of Illinois at Urbana Champaign   (United States)

^e Shanghai Jiao Tong University   (China)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; NUCLEOTIDES; PRECISION ENGINEERING;

AVERAGE FREQUENCY; ENGINEERING METHODS; GENE DISRUPTIONS; GENETIC VARIANTS; GROWTH INHIBITOR; SINGLE NUCLEOTIDES; TARGET SEQUENCES;

YEAST;

ARTICLE; BACTERIAL GENOME; CODON; CONTROLLED STUDY; FRAMESHIFT MUTATION; GENE DELETION; GENE DISRUPTION; GENE EDITING; GENE LOCUS; HYDROGEN BOND; NEXT GENERATION SEQUENCING; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; SINGLE NUCLEOTIDE POLYMORPHISM; AMINO ACID SEQUENCE; BIOTECHNOLOGY; CHEMISTRY; CRISPR CAS SYSTEM; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; FUNGAL GENOME; GENETIC ENGINEERING; GENETICS; METABOLISM; MOLECULAR MODEL; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PROCEDURES; RECOMBINATION REPAIR; VALIDATION STUDY;

FUNGAL DNA; SACCHAROMYCES CEREVISIAE PROTEIN; SIZ1 PROTEIN, S CEREVISIAE; UBIQUITIN PROTEIN LIGASE;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOTECHNOLOGY; CRISPR-CAS SYSTEMS; DIRECTED MOLECULAR EVOLUTION; DNA, FUNGAL; GENE EDITING; GENETIC ENGINEERING; GENOME, FUNGAL; MODELS, MOLECULAR; MUTAGENESIS; RECOMBINATIONAL DNA REPAIR; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; UBIQUITIN-PROTEIN LIGASES;

EID: 85048221553     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.4132     Document Type: Article

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