Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data

PeerJ

Volumn 2018, Issue 5, 2018, Pages

  Palmer, Jonathan M ^a   Jusino, Michelle A ^a   Banik, Mark T ^a   Lindner, Daniel L ^a  

^a USDA FOREST SERVICE   (United States)

Author keywords

Amplicon toolkit; Biodiversity; Environmental sequencing; Fungi; High throughput amplicon sequencing; Metabarcoding; Mycobiome; RRNA internal transcribed spacer; Synthetic spike in control

Indexed keywords

GENOMIC DNA; INTERNAL TRANSCRIBED SPACER; RIBOSOME RNA;

ACCURACY; ALGORITHM; ARTICLE; BIODIVERSITY; BIOINFORMATICS; COMPUTER MODEL; DATA ANALYSIS; DNA EXTRACTION; DNA SEQUENCE; FUNGUS; HIGH THROUGHPUT SEQUENCING; METAGENOMICS; MICROBIAL COMMUNITY; NEXT GENERATION SEQUENCING; NONHUMAN; POLYMERASE CHAIN REACTION; SOFTWARE; TAXONOMY;

EID: 85047606720     PISSN: None     EISSN: 21678359     Source Type: Journal    
DOI: 10.7717/peerj.4925     Document Type: Article

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