A robust CRISPR interference gene repression system in Pseudomonas

Journal of Bacteriology

Volumn 200, Issue 7, 2018, Pages

  Tan, Sue Zanne ^a   Reisch, Christopher R ^b   Prather, Kristala L J ^a  

^a Massachusetts Institute of Technology   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

CRISPRi; Gene repression; Pseudomonas aeruginosa; Pseudomonas fluorescens; Pseudomonas putida

Indexed keywords

BETA GALACTOSIDASE; FTSZ PROTEIN; GLUTAMATE DEHYDROGENASE; GLUTAMATE DEHYDROGENASE (NADP); PYOVERDINE;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL CULTURE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; DNA CLEAVAGE ASSAY; DNA REPLICATION; ENZYME ACTIVITY; ENZYME ASSAY; ESSENTIAL GENE; GENE REPRESSION; GENE TARGETING; GENETIC TRANSCRIPTION; MYCOBACTERIUM SMEGMATIS; NITROGEN AVAILABILITY; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS PUTIDA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; START CODON; STREPTOCOCCUS; STREPTOCOCCUS PASTEURIANUS; TRANSCRIPTION ELONGATION; WESTERN BLOTTING; CRISPR CAS SYSTEM; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; GENOMICS;

BETA-GALACTOSIDASE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; GENE EXPRESSION REGULATION, BACTERIAL; GENE SILENCING; GENOMICS; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS PUTIDA;

EID: 85043512916     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00575-17     Document Type: Article

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