A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: Application for DNA fragment transfer between chromosomes and plasmid transformation

Journal of Microbiological Methods

Volumn 64, Issue 3, 2006, Pages 391-397

  Choi, Kyoung Hee ^a   Kumar, Ayush ^a   Schweizer, Herbert P ^a  

^a Department of Ecosystem Science and Sustainability   (United States)

Author keywords

Competent cells; Electroporation; Mutants; Pseudomonas; Transduction; Transformation

Indexed keywords

BACTERIAL DNA; DNA FRAGMENT;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CONJUGATION; BACTERIUM MUTANT; CENTRIFUGATION; CHROMOSOME MUTATION; DNA TRANSFER; ELECTROPORATION; ELECTROTRANSFORMATION; GENE REPLACEMENT THERAPY; GENETIC TRANSDUCTION; GENETIC TRANSFORMATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; PSEUDOMONAS AERUGINOSA; RELIABILITY; REPRODUCIBILITY; SITE DIRECTED MUTAGENESIS; TRANSPOSON;

CHROMOSOMES, BACTERIAL; DNA, BACTERIAL; ELECTROPORATION; GENE TRANSFER TECHNIQUES; PLASMIDS; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 32244448730     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2005.06.001     Document Type: Article

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