Reliable protein production in a Pseudomonas fluorescens expression system

Protein Expression and Purification

Volumn 81, Issue 2, 2012, Pages 157-165

  Retallack, Diane M ^a   Jin, Hongfan ^a   Chew, Lawrence ^a  

^a Pfenex Inc   (United States)

Author keywords

Protein; Pseudomonas fluorescens; Recombinant

Indexed keywords

PSEUDOMONAS FLUORESCENS;

EID: 80355145782     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.09.010     Document Type: Review

References (72)

1. D.L. Hacker, M. De Jesus, and F.M. Wurm 25 years of recombinant proteins from reactor-grown cells - where do we go from here? Biotechnol. Adv. 27 2009 1023 1027
2. J.A.C. Lim, A. Patkar, G. McDonagh, A. Sinclair, and P. Lucy Process economic benefits of expression technology based on Pseudomonas fluorescens BioProcess Int. 8 2010 62
3. T.D. Landry, L. Chew, J.W. Davis, N. Frawley, H.H. Foley, S.J. Stelman, J. Thomas, J. Wolt, and D.S. Hanselman Safety evaluation of an alpha-amylase enzyme preparation derived from the archaeal order Thermococcales as expressed in Pseudomonas fluorescens biovar I Regul. Toxicol. Pharmacol. 37 2003 149 168 (Pubitemid 36349344)
4. L. Ellingsworth The Use of Pfenex Expression Technology for Large Scale cGMP Production of a Vaccine Antigen, Vaccine Production Summit 2011 IBC Boston, MA
5. M. Silby, A. Cerdeno-Tarraga, G. Vernikos, S. Giddens, R. Jackson, G. Preston, X.-X. Zhang, C. Moon, S. Gehrig, S. Godfrey, C. Knight, J. Malone, Z. Robinson, A. Spiers, S. Harris, G. Challis, A. Yaxley, D. Harris, K. Seeger, L. Murphy, S. Rutter, R. Squares, M. Quail, E. Saunders, K. Mavromatis, T. Brettin, S. Bentley, J. Hothersall, E. Stephens, C. Thomas, J. Parkhill, S. Levy, P. Rainey, and N. Thomson Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens Genome Biol. 10 2009 R51
6. J.E. Loper, D.Y. Kobayashi, and I.T. Paulsen The genomic sequence of Pseudomonas fluorescens Pf-5: insights into biological control Phytopathology 97 2007 233 238 (Pubitemid 46167893)
7. I.T. Paulsen, C.M. Press, J. Ravel, D.Y. Kobayashi, G.S.A. Myers, D.V. Mavrodi, R.T. DeBoy, R. Seshadri, Q. Ren, R. Madupu, R.J. Dodson, A.S. Durkin, L.M. Brinkac, S.C. Daugherty, S.A. Sullivan, M.J. Rosovitz, M.L. Gwinn, L. Zhou, D.J. Schneider, S.W. Cartinhour, W.C. Nelson, J. Weidman, K. Watkins, K. Tran, H. Khouri, E.A. Pierson, L.S. Pierson, L.S. Thomashow, and J.E. Loper Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5 Nat. Biotech. 23 2005 873 878 (Pubitemid 43093131)
8. P. Scholz, V. Haring, B. Wittmann-Liebold, K. Ashman, M. Bagdasarian, and E. Scherzinger Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010 Gene 75 1989 271 288 (Pubitemid 19100128)
9. C. Nieto, E. Fernandez-Tresguerres, N. Sanchez, M. Vicente, and R. Diaz Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas Gene 87 1990 145 149 (Pubitemid 20139867)
10. F. Artiguenave, R. Vilagines, and C. Danglot High-efficiency transposon mutagenesis by electroporation of a Pseudomonas fluorescens strain FEMS Microbiol. Lett. 153 1997 363 369 (Pubitemid 27359733)
11. N. Itoh, T. Kouzai, and Y. Koide Efficient transformation of pseudomonas strains with pNI vectors by electroporation Biosci. Biotechnol. Biochem. 58 1994 1306 1308
12. B. Maestro, J.M. Sanz, R. Diaz-Orejas, and E. Fernandez-Tresguerres Modulation of pPS10 Host Range by Plasmid-Encoded RepA Initiator Protein J. Bacteriol. 185 2003 1367 1375 (Pubitemid 36176869)
13. J.C. Schneider, A.F. Jenings, D.M. Mun, P.M. McGovern, and L.C. Chew Auxotrophic markers pyrF and proC can replace antibiotic markers on protein production plasmids in high-cell-density Pseudomonas fluorescens fermentation Biotechnol. Prog. 21 2005 343 348 (Pubitemid 40466404)
14. E. Alani, L. Cao, and N. Kleckner A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains Genetics 116 1987 541 545
15. T.C. Galvao V. de Lorenzo, Adaptation of the yeast URA3 selection system to gram-negative bacteria and generation of a ΔbetCDE Pseudomonas putida strain Appl. Environ. Microbiol. 71 2005 883 892 (Pubitemid 40223576)
16. K.J. Kwon-Chung, A. Varma, J.C. Edman, and J.E. Bennett Selection of ura5 and ura3 mutants from the two varieties of Cryptococcus neoformans on 5-fluoroorotic acid medium J. Med. Vet. Mycol. 30 1992 61 69
17. Y. Millerioux, M. Clastre, A.J. Simkin, E. Marais, A.A. Sibirny, T. Noel, J. Creche, N. Giglioli-Guivarc'h, and N. Papon Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain J. Microbiol. Methods 84 2011 355 358
18. M.A. Romanos, K.M. Beesley, and J.J. Clare Direct selection of stabilised yeast URA3 transformants with 5-fluorouracil Nucleic Acids Res. 19 1991 187
19. J.P. Woods, D.M. Retallack, E.L. Heinecke, and W.E. Goldman Rare homologous gene targeting in Histoplasma capsulatum: disruption of the URA5Hc gene by allelic replacement J. Bacteriol. 180 1998 5135 5143 (Pubitemid 28470941)
20. M.M. Bagdasarian, E. Amann, R. Lurz, B. Ruckert, and M. Bagdasarian Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors Gene 26 1983 273 282 (Pubitemid 14190480)
21. B. Ghosh, E. Grzadzielska, P. Bhattacharya, E. Peralta, J. DeVito, and A. Das Specificity of antitermination mechanisms. Suppression of the terminator cluster T1-T2 of Escherichia coli ribosomal RNA operon, rrnB, by phage lambda antiterminators J. Mol. Biol. 222 1991 59 66 (Pubitemid 121003988)
22. R.K. Hartmann, P.P. Henze, N. Ulbrich, and V.A. Erdmann Expression of plasmid encoded Escherichia coli 5S ribosomal ribonucleic acid in Pseudomonas putida FEBS Lett. 188 1985 295 301 (Pubitemid 16249747)
23. M.P. Calos, and J.H. Miller The DNA sequence change resulting from the IQ1 mutation, which greatly increases promoter strength Mol. Gen. Genet. 183 1981 559 560 (Pubitemid 12216647)
24. A. Simons, D. Tils, B. von Wilcken-Bergmann, and B. Muller-Hill Possible ideal lac operator: Escherichia coli lac operator-like sequences from eukaryotic genomes lack the central G X C pair Proc. Natl. Acad. Sci. 81 1984 1624 1628 (Pubitemid 14101194)
25. J.C. Schneider, and B. Rosner Mannitol induced promoter systems in bacterial cells, in: US007476532B2 2009 Pfenex Inc. United States
26. D.M. Retallack, T.C. Thomas, Y. Shao, K.L. Haney, S.M. Resnick, V.D. Lee, and C.H. Squires Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions Microb. Cell Fact. 5 2006 1
27. Y.-G. Yang, Q. Tong, W.-D. Zheng, Y.-C. Qian, S.-L. Yang, and Y. Gong The effect of over-expression of chaperones on the secretion and the solubility of proteins Zhongguo Shengwu Huaxue Yu Fenzi Shengwu Xuebao 16 2000 382 387
28. D. Barrick, K. Villanueba, J. Childs, R. Kalil, T.D. Schneider, C.E. Lawrence, L. Gold, and G.D. Stormo Quantitative analysis of ribosome binding sites in E. coli Nucleic Acids Res. 22 1994 1287 1295 (Pubitemid 24162136)
29. D.M. Retallack, J.C. Schneider, J. Mitchell, L. Chew, and H. Liu Transport of heterologous proteins to the periplasmic space of Pseudomonas fluorescens using a variety of native signal sequences Biotechnol. Lett. 29 2007 1483 1491 (Pubitemid 47301355)
30. A. Golden, and D.A. Austen M.R. van Schravendijk, B.J. Sullivan, E.S. Kawasaki, M.S. Osburne, Effect of promoters and signal sequences on the production of secreted HIV-1 gp120 protein in the baculovirus system Protein Expr. Purif. 14 1998 8 12 (Pubitemid 28476574)
31. A.C. Boyd Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids Nucleic Acids Res. 21 1993 817 821 (Pubitemid 23152987)
32. F. Buchholz, and M. Bishop LoxP-directed cloning: use of Cre recombinase as a universal restriction enzyme Biotechniques 31 2001 906 908 910, 912, 914, 916, 918
33. P. Ng, C. Beauchamp, C. Evelegh, R. Parks, and F.L. Graham Development of a FLP/frt system for generating helper-dependent adenoviral vectors Mol. Ther. 3 2001 809 815 (Pubitemid 32519562)
34. N. Radulovich, L. Leung, and M.S. Tsao Modified gateway system for double shRNA expression and Cre/lox based gene expression BMC Biotechnol. 11 1998 24
35. J. Seibler, D. Schubeler, S. Fiering, M. Groudine, and J. Bode DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs Biochemistry 37 1998 6229 6234 (Pubitemid 28213639)
36. T. Perehinec, S. Qazi, S. Gaddipati, V. Salisbury, C. Rees, and P. Hill Construction and evaluation of multisite recombinatorial (Gateway) cloning vectors for Gram-positive bacteria BMC Mol. Biol. 8 2007 80
37. K. Kwon, J. Hasseman, S. Latham, C. Grose, Y. Do, R. Fleischmann, R. Pieper, and S. Peterson Recombinant expression and functional analysis of proteases from Streptococcus pneumoniae, Bacillus anthracis, and Yersinia pestis BMC Biochemistry 12 2011 17
38. W. Szybalski Universal restriction endonucleases: designing novel cleavage specificities by combining adapter oligodeoxynucleotide and enzyme moieties Gene 40 1985 169 173 (Pubitemid 16107671)
39. W. Szybalski, S.C. Kim, N. Hasan, and A.J. Podhajska Class-IIS restriction enzymes-a review Gene 100 1991 13 26
40. F. Baneyx, and G. Georgiou Construction and characterization of Escherichia coli strains deficient in multiple secreted proteases: protease III degrades high-molecular-weight substrates in vivo J. Bacteriol. 173 1991 2696 2703
41. S.J. Park, G. Georgiou, and S.Y. Lee Secretory production of recombinant protein by a high cell density culture of a protease negative mutant Escherichia coli strain Biotechnol. Prog. 15 1999 164 167 (Pubitemid 29174931)
42. L.C. Simmons, D. Reilly, L. Klimowski, T.S. Raju, G. Meng, P. Sims, K. Hong, R.L. Shields, L.A. Damico, P. Rancatore, and D.G. Yansura Expression of full-length immunoglobulins in Escherichia coli: rapid and efficient production of aglycosylated antibodies J. Immunol. Methods 263 2002 133 147 (Pubitemid 34493816)
43. J.G. Thomas, A. Ayling, and F. Baneyx Molecular chaperones, folding catalysts, and the recovery of active recombinant proteins from E. coli. To fold or to refold Appl. Biochem. Biotechnol. 66 1997 197 238 (Pubitemid 127499124)
44. F. Baneyx Recombinant protein expression in Escherichia coli Curr. Opin. Biotechnol. 10 1999 411 421 (Pubitemid 29457264)
45. N. Narayanan, M. Khan, and C.P. Chou Enhancing functional expression of Heterologous Burkholderia lipase in Escherichia coli Mol. Biotechnol. 47 2011 130 143
46. Y. Chen, J. Song, S.F. Sui, and D.N. Wang DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli Protein. Expr. Purif. 32 2003 221 231 (Pubitemid 38039013)
47. K. Nishihara, M. Kanemori, M. Kitagawa, H. Yanagi, and T. Yura Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli Appl. Environ. Microbiol. 64 1998 1694 1699 (Pubitemid 28234771)
48. S.C. Lee, and P.O. Olins Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli J. Biol. Chem. 267 1992 2849 2852
49. Z. Zhang, Z.H. Li, F. Wang, M. Fang, C.C. Yin, Z.Y. Zhou, Q. Lin, and H.L. Huang Overexpression of DsbC and DsbG markedly improves soluble and functional expression of single-chain Fv antibodies in Escherichia coli Protein Expr. Purif. 26 2002 218 228 (Pubitemid 35326539)
50. C.-H. Luan, S. Qiu, J.B. Finley, M. Carson, R.J. Gray, W. Huang, D. Johnson, J. Tsao, J.R.M. Reboul, P. Vaglio, D.E. Hill, M. Vidal, L.J. DeLucas, and M. Luo High-Throughput Expression of C. elegans Proteins Genome Res. 14 2004 2102 2110 (Pubitemid 39440192)
51. S.P. Chambers, D.A. Austen, J.R. Fulghum, and W.M. Kim High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells Protein Expr. Purif. 36 2004 40 47 (Pubitemid 38836762)
52. A. Ergin, K. Bussow, J. Sieper, A. Thiel, R. Duchmann, and T. Adam Homologous high-throughput expression and purification of highly conserved E. coli proteins Microb. Cell Fact. 6 2007 18
53. Y. Benita, M.J. Wise, M.C. Lok, I. Humphery-Smith, and R.S. Oosting Analysis of high throughput protein expression in Escherichia coli Mol. Cell. Proteomics 5 2006 1567 1580 (Pubitemid 44480386)
54. P. Braun, Y. Hu, B. Shen, A. Halleck, M. Koundinya, E. Harlow, and J. LaBaer Proteome-scale purification of human proteins from bacteria Proc. Natl. Acad. Sci. USA 99 2002 2654 2659 (Pubitemid 34240516)
55. Y.J. Tang-Feldman, G. Ackermann, J.P. Henderson, J. Silva Jr., and S.H. Cohen One-step cloning and expression of Clostridium difficile toxin B gene (tcdB) Mol. Cell Probes 16 2002 179 183
56. D.E. Voth, and J.D. Ballard Clostridium difficile toxins: mechanism of action and role in disease Clin. Microbiol. Rev. 18 2005 247 263 (Pubitemid 40548293)
57. D.M. Lyerly, H.C. Krivan, and T.D. Wilkins Clostridium difficile: its disease and toxins Clin. Microbiol. Rev. 1 1988 1 18
58. D. Albesa-Jove, T. Bertrand, E.P. Carpenter, G.V. Swain, J. Lim, J. Zhang, L.F. Haire, N. Vasisht, V. Braun, A. Lange, C. von Eichel-Streiber, D.I. Svergun, N.F. Fairweather, and K.A. Brown Four distinct structural domains in Clostridium difficile toxin B visualized using SAXS J. Mol. Biol. 396 2010 1260 1270
59. K.L. Kotloff, S.S. Wasserman, G.A. Losonsky, W. Thomas Jr., R. Nichols, R. Edelman, M. Bridwell, and T.P. Monath Safety and immunogenicity of increasing doses of a Clostridium difficile toxoid vaccine administered to healthy adults Infect. Immun. 69 2001 988 995 (Pubitemid 32109565)
60. A.C.A. Roque, C.R. Lowe, and M.Â. Taipa Antibodies and genetically engineered related molecules: production and purification Biotechnol. Prog. 20 2004 639 654 (Pubitemid 38784002)
61. G. Klima Expression of an antibody fragment utilizing the Pfenex Expression Technology™ platform, Difficult to Express Proteins PEGS 2011 2011 CHI Boston, MA
62. D.C. Montgomery, Design and Analysis of Experiments, John Wiley and Sons, 2005.
63. N. Yokoyama, M. Hirata, K. Ohtsuka, Y. Nishiyama, K. Fujii, M. Fujita, K. Kuzushima, T. Kiyono, T. Tsurumi, Co-expression of human chaperone Hsp70 and Hsdj or Hsp40 co-factor increases solubility of overexpressed target proteins in insect cells. Biochimica et Biophysica Acta (BBA) - Gene Struct. Expr. 1493 (2000) 119-124.
64. T. Ahn, S. Yang, and C.-H. Yun High-level expression of human cytochrome P450 1A2 by co-expression with human molecular chaperone HDJ-1(Hsp40) Protein Expr. Purif. 36 2004 48 52 (Pubitemid 38836763)
65. D.P. Humphreys, N. Weir, A. Lawson, A. Mountain, and P.A. Lund Co-expression of human protein disulphide isomerase (PDI) can increase the yield of an antibody Fab' fragment expressed in Escherichia coli FEBS Lett. 380 1996 194 197 (Pubitemid 26058757)
66. X. Zhan, M. Schwaller, H.F. Gilbert, and G. Georgiou Facilitating the formation of disulfide bonds in the Escherichia coli periplasm via coexpression of yeast protein disulfide isomerase Biotechnol. Prog. 15 1999 1033 1038
67. H. Jin, G.T. Cantin, S. Maki, L.C. Chew, S.M. Resnick, J. Ngai, and D.M. Retallack Soluble periplasmic production of human granulocyte colony-stimulating factor (G-CSF) in Pseudomonas fluorescens Protein Expr. Purif. 78 2011 69 77
68. A.L. Vanz, G. Renard, M.S. Palma, J.M. Chies, S.L. Dalmora, L.A. Basso, and D.S. Santos Human granulocyte colony stimulating factor (hG-CSF): cloning, overexpression, purification and characterization Microb. Cell Fact. 7 2008 13
69. J.P. Phelps, N. Dang, and L. Rasochova Inactivation and purification of cowpea mosaic virus-like particles displaying peptide antigens from Bacillus anthracis J. Virol. Methods 141 2007 146 153 (Pubitemid 46476811)
70. X. Zhao, J.M. Fox, N.H. Olson, T.S. Baker, and M.J. Young In vitro assembly of cowpea chlorotic mottle virus from coat protein expressed in Escherichia coli and in vitro-transcribed viral cDNA Virology 207 1995 486 494
71. G. Wu, C. Feng, Y. Hong, A. Guo, S. Cao, J. Dong, L. Lin, and Z. Liu Soluble expression and purification of the anthrax protective antigen in E coli and identification of a novel dominant-negative mutant N435C Appl. Microbiol. Biotechnol. 87 2010 609 616
72. S.C. Haslam, J.M. Ketley, T.J. Mitchell, J. Stephen, D.W. Burdon, and D.C. Candy Growth of Clostridium difficile and production of toxins A and B in complex and defined media J. Med. Microbiol. 21 1986 293 297 (Pubitemid 16053517)