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This paper summarizes the production of bioplastics polyhydroxyalkanoates (PHA), ectoines, enzymes, and bio-surfactants via halophiles. And the advances in molecular manipulation for halophilic microorganisms.
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Yin, J., Chen, J.C., Wu, Q., Chen, G.Q., Halomonas spp., a rising star for industrial biotechnology. Biotechnol Adv 33 (2015), 1433–1442 This paper summarizes the production of bioplastics polyhydroxyalkanoates (PHA), ectoines, enzymes, and bio-surfactants via halophiles. And the advances in molecular manipulation for halophilic microorganisms.
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Biotechnol Adv
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Yin, J.1
Chen, J.C.2
Wu, Q.3
Chen, G.Q.4
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45
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85026914877
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Engineering Halomonas bluephagenesis TD01 for unsterile production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)
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By integrating orfZ gene encoding 4HB-CoA transferase into the chromosome, Halomonas bluephagenesis TD01 was engineered to produce 70–80 g/l cell biomass with >60% P(3HB-co-16 mol% 4HB) in fermentors. And the non-sterile fermentations were scaled up from 1, 7 to 1000-L with similar results.
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Chen, X.B., Jin, Y., Ye, J.W., Zhang, H.Q., Che, X.M., Ma, Y.M., Li, M.Y., Chen, G.Q., Engineering Halomonas bluephagenesis TD01 for unsterile production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate). Biores Technol 244 (2017), 534–541 By integrating orfZ gene encoding 4HB-CoA transferase into the chromosome, Halomonas bluephagenesis TD01 was engineered to produce 70–80 g/l cell biomass with >60% P(3HB-co-16 mol% 4HB) in fermentors. And the non-sterile fermentations were scaled up from 1, 7 to 1000-L with similar results.
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(2017)
Biores Technol
, vol.244
, pp. 534-541
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Chen, X.B.1
Jin, Y.2
Ye, J.W.3
Zhang, H.Q.4
Che, X.M.5
Ma, Y.M.6
Li, M.Y.7
Chen, G.Q.8
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46
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80055030247
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Comparative genomics study of PHA and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships
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Cai, L., Tan, D., Aibaidula, G., Dong, X.R., Chen, J.C., Tian, W.D., Chen, G.Q., Comparative genomics study of PHA and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships. Microb Cell Fact, 10, 2011, 88.
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(2011)
Microb Cell Fact
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, pp. 88
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Cai, L.1
Tan, D.2
Aibaidula, G.3
Dong, X.R.4
Chen, J.C.5
Tian, W.D.6
Chen, G.Q.7
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47
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84904535022
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A sea water based open and continuous process for polyhydroxyalkanoates production by Halomonas campaniensis LS21
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Yue, H.T., Chen, X.B., Ling, C., Chen, Y.L., Deng, H.T., Chen, G.Q., A sea water based open and continuous process for polyhydroxyalkanoates production by Halomonas campaniensis LS21. Biotechnol Biofuels, 7, 2014, 108.
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(2014)
Biotechnol Biofuels
, vol.7
, pp. 108
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Yue, H.T.1
Chen, X.B.2
Ling, C.3
Chen, Y.L.4
Deng, H.T.5
Chen, G.Q.6
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48
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84907856997
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Engineering Halomonas TD01 for low cost production of polyhydroxyalkanoates
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Tan, D., Wu, Q., Chen, J.C., Chen, G.Q., Engineering Halomonas TD01 for low cost production of polyhydroxyalkanoates. Metab Eng 26 (2014), 34–47.
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(2014)
Metab Eng
, vol.26
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Tan, D.1
Wu, Q.2
Chen, J.C.3
Chen, G.Q.4
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49
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84920257993
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Development of an enhanced chromosomal expression system based on porin synthesis operon in Halomonas TD01
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Yin, J., Fu, X.Z., Wu, Q., Chen, J.C., Chen, G.Q., Development of an enhanced chromosomal expression system based on porin synthesis operon in Halomonas TD01. Appl Microbiol Biotechnol 98 (2014), 8987–8997.
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(2014)
Appl Microbiol Biotechnol
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, pp. 8987-8997
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Yin, J.1
Fu, X.Z.2
Wu, Q.3
Chen, J.C.4
Chen, G.Q.5
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50
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85017124187
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CRISPRi engineering Halomonas for polyhydroxyalkanoates (PHA) synthesis
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Tao, W., Lv, L., Li, D., Chen, J.C., Chen, G.Q., CRISPRi engineering Halomonas for polyhydroxyalkanoates (PHA) synthesis. Microb Cell Fact, 16, 2017, 48.
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(2017)
Microb Cell Fact
, vol.16
, pp. 48
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Tao, W.1
Lv, L.2
Li, D.3
Chen, J.C.4
Chen, G.Q.5
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51
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85007477352
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Novel T7-like expression systems used for Halomonas
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In this paper, novel T7-like expression systems were developed for non-model bacteria including Halomonas and Pseudomonas entomophila. Ultrahigh PHB accumulation (92% of CDW) was achieved in Halomonas TD01 using T7-like MmP1.
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Zhao, H., Zhang, H.Q.M., Li, T., Lou, C.B., Ouyang, Q., Chen, G.Q., Novel T7-like expression systems used for Halomonas. Metab Eng 39 (2017), 128–140 In this paper, novel T7-like expression systems were developed for non-model bacteria including Halomonas and Pseudomonas entomophila. Ultrahigh PHB accumulation (92% of CDW) was achieved in Halomonas TD01 using T7-like MmP1.
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(2017)
Metab Eng
, vol.39
, pp. 128-140
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Zhao, H.1
Zhang, H.Q.M.2
Li, T.3
Lou, C.B.4
Ouyang, Q.5
Chen, G.Q.6
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52
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84978068992
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Engineering the growth pattern and cell morphology for enhanced PHB production by Escherichia coli
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Wu, H., Chen, J.C., Chen, G.Q., Engineering the growth pattern and cell morphology for enhanced PHB production by Escherichia coli. Appl Microbiol Biotechnol 100 (2016), 9907–9916.
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(2016)
Appl Microbiol Biotechnol
, vol.100
, pp. 9907-9916
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Wu, H.1
Chen, J.C.2
Chen, G.Q.3
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53
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84997013161
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Engineering Halomonas spp. as a low-cost production host for production of bio-surfactant protein PhaP
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In this paper, Halomonas strain TD is used to produce a protein named PHA phasin or PhaP which has a potential to be developed into a bio-surfactant.
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Lan, L.H., Zhao, H., Chen, J.C., Chen, G.Q., Engineering Halomonas spp. as a low-cost production host for production of bio-surfactant protein PhaP. Biotechnol J 11 (2016), 1595–1604 In this paper, Halomonas strain TD is used to produce a protein named PHA phasin or PhaP which has a potential to be developed into a bio-surfactant.
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(2016)
Biotechnol J
, vol.11
, pp. 1595-1604
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Lan, L.H.1
Zhao, H.2
Chen, J.C.3
Chen, G.Q.4
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54
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85010021893
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Microbial synthesis of 5-aminolevulinic acid (ALA) and its co-production with polyhydroxybutyrate (PHB)
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This paper reviews the recent strategies developed in PHA co-production with other compounds, discusses the challenges and prospective during the scale up of the co-production strategies.
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Li, T., Guo, Y.Y., Qiao, G.Q., Wu, Q., Chen, G.Q., Microbial synthesis of 5-aminolevulinic acid (ALA) and its co-production with polyhydroxybutyrate (PHB). ACS Syn Biol 5 (2016), 1264–1275 This paper reviews the recent strategies developed in PHA co-production with other compounds, discusses the challenges and prospective during the scale up of the co-production strategies.
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(2016)
ACS Syn Biol
, vol.5
, pp. 1264-1275
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Li, T.1
Guo, Y.Y.2
Qiao, G.Q.3
Wu, Q.4
Chen, G.Q.5
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