Systematic Approaches to Efficiently Produce 2,3-Butanediol in a Marine Cyanobacterium

ACS Synthetic Biology

Volumn 6, Issue 11, 2017, Pages 2136-2144

  Nozzi, Nicole E ^a   Case, Anna E ^a   Carroll, Austin L ^a   Atsumi, Shota ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

2,3 butanediol; cyanobacteria; marine cyanobacteria; metabolic engineering

Indexed keywords

2,3 BUTANEDIOL; CHLOROPHYLL A; DEOXYRIBONUCLEASE; DNA POLYMERASE; ISOPROPYL THIOGALACTOSIDE; RESTRICTION ENDONUCLEASE; THEOPHYLLINE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; 2,3-BUTYLENE GLYCOL; BUTANEDIOL;

ARTICLE; BACTERIAL GROWTH; CELL DENSITY; CONCENTRATION PROCESS; CONTROLLED STUDY; CYANOBACTERIUM; GENE EXPRESSION; GENETIC ENGINEERING; GROWTH RATE; HOMOLOGOUS RECOMBINATION; MARINE BACTERIUM; NONHUMAN; NUTRIENT CONCENTRATION; OPERON; PHOTOSYNTHESIS; PRIORITY JOURNAL; PROMOTER REGION; RIBOSWITCH; SCALE UP; SYNECHOCOCCUS ELONGATUS; AQUATIC SPECIES; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES; SYNECHOCOCCUS;

AQUATIC ORGANISMS; BUTYLENE GLYCOLS; METABOLIC ENGINEERING; SYNECHOCOCCUS;

EID: 85034589995     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.7b00157     Document Type: Article

References (39)

1. Case, A. E. and Atsumi, S. (2016) Cyanobacterial chemical production J. Biotechnol. 231, 106-114 10.1016/j.jbiotec.2016.05.023
2. 2 to ethylene in recombinant cyanobacterium Synechocystis 6803 Energy Environ. Sci. 5, 8998-9006 10.1039/c2ee22555g
3. Oliver, J. W., Machado, I. M., Yoneda, H., and Atsumi, S. (2013) Cyanobacterial conversion of carbon dioxide to 2,3-butanediol Proc. Natl. Acad. Sci. U. S. A. 110, 1249-1254 10.1073/pnas.1213024110
4. Liu, X., Sheng, J., and Curtiss, R., 3rd (2011) Fatty acid production in genetically modified cyanobacteria Proc. Natl. Acad. Sci. U. S. A. 108, 6899-6904 10.1073/pnas.1103014108
5. Chisti, Y. (2013) Constraints to commercialization of algal fuels J. Biotechnol. 167, 201-214 10.1016/j.jbiotec.2013.07.020
6. Shiklomanov, I. A. (1993) Water in Crisis: A Guide to the World's Fresh Water Resources, Oxford University Press.
7. Batterton, J. C., Jr. and Van Baalen, C. (1971) Growth responses of blue-green algae to sodium chloride concentration Arch. Microbiol. 76, 151-165 10.1007/BF00411789
8. Xu, Y., Alvey, R., Byrne, P., Graham, J., Shen, G., and Bryant, D. (2011) Expression of genes in cyanobacteria: Adaptation of endogenous plasmids as platforms for high-level gene expression in Synechococcus sp. PCC 7002, In Photosynthesis Research Protocols (Carpentier, R., Ed.), pp 273-293, Humana Press.
9. Nomura, C. T., Sakamoto, T., and Bryant, D. A. (2006) Roles for heme-copper oxidases in extreme high-light and oxidative stress response in the cyanobacterium Synechococcus sp. PCC 7002 Arch. Microbiol. 185, 471-479 10.1007/s00203-006-0107-7
10. Ludwig, M. and Bryant, D. A. (2012) Synechococcus sp. Strain PCC 7002 transcriptome: Acclimation to temperature, salinity, oxidative stress, and mixotrophic growth conditions Front. Microbiol. 3, 354 10.3389/fmicb.2012.00354
11. Stevens, S. E. and Porter, R. D. (1980) Transformation in Agmenellum quadruplicatum Proc. Natl. Acad. Sci. U. S. A. 77, 6052-6056 10.1073/pnas.77.10.6052
12. Frigaard, N. U., Sakuragi, Y., and Bryant, D. A. (2004) Gene inactivation in the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation Methods Mol. Biol. 274, 325-340 10.1385/1-59259-799-8:325
13. Markley, A. L., Begemann, M. B., Clarke, R. E., Gordon, G. C., and Pfleger, B. F. (2015) Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002 ACS Synth. Biol. 4, 595-603 10.1021/sb500260k
14. 2+-inducible expression platform for Synechococcus sp. strain PCC 7002 based on the smtA promoter/operator and smtB repressor Appl. Environ. Microbiol. 83, e02491-02416 10.1128/AEM.02491-16
15. Begemann, M. B., Zess, E. K., Walters, E. M., Schmitt, E. F., Markley, A. L., and Pfleger, B. F. (2013) An organic acid based counter selection system for cyanobacteria PLoS One 8, e76594 10.1371/journal.pone.0076594
16. Ruffing, A. M., Jensen, T. J., and Strickland, L. M. (2016) Genetic tools for advancement of Synechococcus sp. PCC 7002 as a cyanobacterial chassis Microb. Cell Fact. 15, 190 10.1186/s12934-016-0584-6
17. Gordon, G. C., Korosh, T. C., Cameron, J. C., Markley, A. L., Begemann, M. B., and Pfleger, B. F. (2016) CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002 Metab. Eng. 38, 170-179 10.1016/j.ymben.2016.07.007
18. Kopka, J., Schmidt, S., Dethloff, F., Pade, N., Berendt, S., Schottkowski, M., Martin, N., Duhring, U., Kuchmina, E., Enke, H., Kramer, D., Wilde, A., Hagemann, M., and Friedrich, A. (2017) Systems analysis of ethanol production in the genetically engineered cyanobacterium Synechococcus sp. PCC 7002 Biotechnol. Biofuels 10, 56 10.1186/s13068-017-0741-0
19. Zhang, S., Liu, Y., and Bryant, D. A. (2015) Metabolic engineering of Synechococcus sp. PCC 7002 to produce poly-3-hydroxybutyrate and poly-3-hydroxybutyrate-co-4-hydroxybutyrate Metab. Eng. 32, 174-183 10.1016/j.ymben.2015.10.001
20. Ruffing, A. M. (2014) Improved free fatty acid production in cyanobacteria with Synechococcus sp. PCC 7002 as host Front. Bioeng. Biotechnol. 2, 17 10.3389/fbioe.2014.00017
21. Davies, F. K., Work, V. H., Beliaev, A. S., and Posewitz, M. C. (2014) Engineering limonene and bisabolene production in wild yype and a glycogen-deficient mutant of Synechococcus sp. PCC 7002 Front. Bioeng. Biotechnol. 2, 21 10.3389/fbioe.2014.00021
22. 2 in a cyanobacterium Metab. Eng. 21, 60-70 10.1016/j.ymben.2013.11.004
23. Iwai, M., Katoh, H., Katayama, M., and Ikeuchi, M. (2004) Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1 Plant Cell Physiol. 45, 171-175 10.1093/pcp/pch015
24. Pinto, A. V., Mathieu, A., Marsin, S., Veaute, X., Ielpi, L., Labigne, A., and Radicella, J. P. (2005) Suppression of homologous and homeologous recombination by the bacterial MutS2 protein Mol. Cell 17, 113-120 10.1016/j.molcel.2004.11.035
25. Pedelacq, J. D., Cabantous, S., Tran, T., Terwilliger, T. C., and Waldo, G. S. (2006) Engineering and characterization of a superfolder green fluorescent protein Nat. Biotechnol. 24, 79-88 10.1038/nbt1172
26. Nozzi, N. E. and Atsumi, S. (2015) Genome engineering of the 2,3-butanediol biosynthetic pathway for tight regulation in cyanobacteria ACS Synth. Biol. 4, 1197-1204 10.1021/acssynbio.5b00057
27. Nakahira, Y., Ogawa, A., Asano, H., Oyama, T., and Tozawa, Y. (2013) Theophylline-dependent riboswitch as a novel genetic tool for strict regulation of protein expression in Cyanobacterium Synechococcus elongatus PCC 7942 Plant Cell Physiol. 54, 1724-1735 10.1093/pcp/pct115
28. Topp, S., Reynoso, C. M., Seeliger, J. C., Goldlust, I. S., Desai, S. K., Murat, D., Shen, A., Puri, A. W., Komeili, A., Bertozzi, C. R., Scott, J. R., and Gallivan, J. P. (2010) Synthetic riboswitches that induce gene expression in diverse bacterial species Appl. Environ. Microbiol. 76, 7881-7884 10.1128/AEM.01537-10
29. Xu, Y., Alvey, R. M., Byrne, P. O., Graham, J. E., Shen, G., and Bryant, D. A. (2011) Expression of genes in cyanobacteria: adaptation of endogenous plasmids as platforms for high-level gene expression in Synechococcus sp. PCC 7002 Methods Mol. Biol. 684, 273-293 10.1007/978-1-60761-925-3-21
30. 2 fixation and chemical production in cyanobacteria Nat. Commun. 8, 14724 10.1038/ncomms14724
31. Stevens, S. E., Patterson, C. O. P., and Myers, J. (1973) The Production of Hydrogen Peroxide by Blue-Green Algae: A Survey J. Phycol. 9, 427-430 10.1111/j.1529-8817.1973.tb04116.x
32. Mattam, A. J., Clomburg, J. M., Gonzalez, R., and Yazdani, S. S. (2013) Fermentation of glycerol and production of valuable chemical and biofuel molecules Biotechnol. Lett. 35, 831-842 10.1007/s10529-013-1240-4
33. Kanno, M. and Atsumi, S. (2017) Engineering an obligate photoautotrophic cyanobacterium to utilize glycerol for growth and chemical production ACS Synth. Biol. 6, 69-75 10.1021/acssynbio.6b00239
34. McEwen, J. T., Kanno, M., and Atsumi, S. (2016) 2,3 Butanediol production in an obligate photoautotrophic cyanobacterium in dark conditions via diverse sugar consumption Metab. Eng. 36, 28-36 10.1016/j.ymben.2016.03.004
35. Li, M. Z. and Elledge, S. J. (2007) Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC Nat. Methods 4, 251-256 10.1038/nmeth1010
36. Voges, O. and Proskauer, B. (1898) Beitraege zur Ernaehrungsphysiologie und zur Differential Diagnose der Bakterien der hemmorrhagischen Septicamie Med. Microbiol. Immunol. 28, 20-32 10.1007/BF02285362
37. Westerfield, W. W. (1945) A colorimetric determination of blood acetoin J. Biol. Chem. 161, 495-502
38. Oliver, J. W., Machado, I. M., Yoneda, H., and Atsumi, S. (2014) Combinatorial optimization of cyanobacterial 2,3-butanediol production Metab. Eng. 22, 76-82 10.1016/j.ymben.2014.01.001
39. Porra, R. J., Thompson, W. A., and Kriedemann, P. E. (1989) Determination of accurate extinction coefficients and simultaneous-equations for assaying Chlorophyll-a and Chlorophyll-b extracted with 4 different solvents-Verification of the concentration of chlorophyll standards by Atomic-Absorption Spectroscopy Biochim. Biophys. Acta, Bioenerg. 975, 384-394 10.1016/S0005-2728(89)80347-0