Simultaneous detection of NADPH consumption and H 2 O 2 production using the Ampliflu™ Red assay for screening of P450 activities and uncoupling

Applied Microbiology and Biotechnology

Volumn 102, Issue 2, 2018, Pages 985-994

  Morlock, Lisa K ^a   Böttcher, Dominique ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Cytochrome P450; Hydrogen peroxide; N acetyl 3,7 dihydroxyphenoxazin; P450 BM3; Uncoupling

Indexed keywords

BACTERIOLOGY; ENZYMES; HYDROGEN PEROXIDE; IRON COMPOUNDS; OXIDATION; PEROXIDES; PORPHYRINS; SUBSTRATES;

ACTIVATED OXYGEN SPECIES; CYTOCHROME P450; MICROTITER-PLATE BASED ASSAYS; N ACETYLS; P450 BM3; REACTIVE OXYGEN SPECIES; SIMULTANEOUS DETECTION; UNCOUPLING;

MOLECULAR OXYGEN;

CYTOCHROME P450; HYDROGEN PEROXIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNSPECIFIC MONOOXYGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

BACTERIUM; BIOASSAY; CATALYSIS; CYTOCHROME; ENZYME; ENZYME ACTIVITY; HYDROGEN; HYDROGEN PEROXIDE; IRON; REACTIVE OXYGEN SPECIES; SUBSTRATE;

ARTICLE; BACILLUS MEGATERIUM; GAS CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICROTITER PLATE ASSAY; NONHUMAN; PH; CATALYSIS; ELECTRON TRANSPORT; ENZYME ASSAY; ENZYMOLOGY; KINETICS; METABOLISM; OXIDATION REDUCTION REACTION;

BACILLUS MEGATERIUM;

BACILLUS MEGATERIUM; CATALYSIS; CYTOCHROME P-450 ENZYME SYSTEM; ELECTRON TRANSPORT; ENZYME ASSAYS; HYDROGEN PEROXIDE; KINETICS; NAD; NADP; NADPH-FERRIHEMOPROTEIN REDUCTASE; OXIDATION-REDUCTION; REACTIVE OXYGEN SPECIES;

EID: 85034217962     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-017-8636-3     Document Type: Article

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