Assessment of the multiplex PCR-based assay Unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates

Infection

Volumn 46, Issue 2, 2018, Pages 189-196

  Papan, Cihan ^a,b   Meyer Buehn, Melanie ^a   Laniado, Gudrun ^a   Nicolai, Thomas ^a   Griese, Matthias ^a   Huebner, Johannes ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Bacterial pneumonia; Bronchoalveolar lavage; Children; Multiplex PCR; Neonates

Indexed keywords

ACYLAMINOPENICILLIN; BETA LACTAM ANTIBIOTIC; BETA LACTAMASE CTX M; BETA LACTAMASE SHV; CARBAPENEM DERIVATIVE; CEPHALOSPORIN DERIVATIVE; QUINOLONE DERIVATIVE; UNCLASSIFIED DRUG;

ACINETOBACTER BAUMANNII; ANTIBIOTIC RESISTANCE; ANTIBIOTIC RESISTANCE GENE; ARTICLE; ASSAY; BACTERIAL PNEUMONIA; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BRONCHOALVEOLAR LAVAGE FLUID; BRONCHOSCOPY; CHILD; CHLAMYDIA PNEUMONIAE; DIAGNOSTIC ACCURACY; EBC GENE; ENTEROBACTER; ERMB GENE; ERMC GENE; ESCHERICHIA COLI; FEMALE; GENE; GRAM POSITIVE BACTERIUM; GYRA87 GENE; HAEMOPHILUS INFLUENZAE; HUMAN; INT1 GENE; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; LEGIONELLA PNEUMOPHILA; MAJOR CLINICAL STUDY; MALE; MECA GENE; MEFA GENE; MICROORGANISM DETECTION; MORAXELLA CATARRHALIS; MORGANELLA MORGANII; MULTIPLEX POLYMERASE CHAIN REACTION; NEWBORN; NON-FERMENTING BACTERIUM; OBSERVATIONAL STUDY; PLEURA FLUID; PNEUMOCYSTIS JIROVECI; PREDICTIVE VALUE; PROSPECTIVE STUDY; PROTEUS; PSEUDOMONAS AERUGINOSA; SENSITIVITY AND SPECIFICITY; SERRATIA MARCESCENS; STAPHYLOCOCCUS AUREUS; STENOTROPHOMONAS MALTOPHILIA; STREPTOCOCCUS PNEUMONIAE; SUL1 GENE; TEM GENE; TRACHEAL ASPIRATION PROCEDURE; UNYVERO ASSAY; ADOLESCENT; ADULT; BACTERIUM; CLASSIFICATION; CROSS INFECTION; GENETICS; INFANT; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR TYPING; PRESCHOOL CHILD; PROCEDURES; YOUNG ADULT;

ADOLESCENT; ADULT; BACTERIA; BRONCHOALVEOLAR LAVAGE FLUID; CHILD; CHILD, PRESCHOOL; CROSS INFECTION; DRUG RESISTANCE, BACTERIAL; FEMALE; HUMANS; INFANT; INFANT, NEWBORN; MALE; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; PNEUMONIA, BACTERIAL; PROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 85032675080     PISSN: 03008126     EISSN: 14390973     Source Type: Journal    
DOI: 10.1007/s15010-017-1088-y     Document Type: Article

References (41)

1. Liu L, Oza S, Hogan D, Chu Y, Perin J, Zhu J, et al. Global, regional, and national causes of under-5 mortality in 2000-15: an updated systematic analysis with implications for the Sustainable Development Goals. Lancet (London, England). 2017;388:3027–35. https://doi.org/10.1016/s0140-6736(16)31593-8.
2. Jain S, Williams DJ, Arnold SR, Ampofo K, Bramley AM, Reed C, et al. Community-acquired pneumonia requiring hospitalization among US children. New Engl J Med. 2015;372(9):835–45. https://doi.org/10.1056/NEJMoa1405870.
3. Harris M, Clark J, Coote N, Fletcher P, Harnden A, McKean M, et al. British Thoracic Society guidelines for the management of community acquired pneumonia in children: update 2011. Thorax. 2011;66:ii1–23. https://doi.org/10.1136/thoraxjnl-2011-200598.
4. Bradley JS, Byington CL, Shah SS, Alverson B, Carter ER, Harrison C, et al. The management of community-acquired pneumonia in infants and children older than 3 months of age: clinical practice guidelines by the Pediatric Infectious Diseases Society and the Infectious Diseases Society of America. Clin Infect Dis: Off Public Infect Dis Soc Am. 2011;53:e25–76. https://doi.org/10.1093/cid/cir531.
5. Chang I, Schibler A. Ventilator associated pneumonia in children. Paediatr Respir Rev. 2016;20:10–6. https://doi.org/10.1016/j.prrv.2015.09.005.
6. Cernada M, Brugada M, Golombek S, Vento M. Ventilator-associated pneumonia in neonatal patients: an update. Neonatology. 2014;105:98–107. https://doi.org/10.1159/000355539.
7. Guidelines for the management of adults with hospital-acquired. ventilator-associated, and healthcare-associated pneumonia. Am J Respir Crit Care Med. 2005;171:388–416. https://doi.org/10.1164/rccm.200405-644ST.
8. Edwards JR, Peterson KD, Andrus ML, Dudeck MA, Pollock DA, Horan TC. National Healthcare Safety Network (NHSN) Report, data summary for 2006 through 2007, issued November 2008. Am J Infect Control. 2008;36:609–26. https://doi.org/10.1016/j.ajic.2008.08.001.
9. Hunter JD. Ventilator associated pneumonia. BMJ. 2012;344:e3325. https://doi.org/10.1136/bmj.e3325.
10. Holter JC, Muller F, Bjorang O, Samdal HH, Marthinsen JB, Jenum PA, et al. Etiology of community-acquired pneumonia and diagnostic yields of microbiological methods: a 3-year prospective study in Norway. BMC Infect Dis. 2015;15:64. https://doi.org/10.1186/s12879-015-0803-5.
11. Gies F, Tschiedel E, Felderhoff-Muser U, Rath PM, Steinmann J, Dohna-Schwake C. Prospective evaluation of SeptiFast Multiplex PCR in children with systemic inflammatory response syndrome under antibiotic treatment. BMC Infect Dis. 2016;16:378. https://doi.org/10.1186/s12879-016-1722-9.
12. Schlueter M, James C, Dominguez A, Tsu L, Seymann G. Practice patterns for antibiotic de-escalation in culture-negative healthcare-associated pneumonia. Infection. 2010;38:357–62. https://doi.org/10.1007/s15010-010-0042-z.
13. Vincent JL, Rello J, Marshall J, Silva E, Anzueto A, Martin CD, et al. International study of the prevalence and outcomes of infection in intensive care units. JAMA. 2009;302:2323–9. https://doi.org/10.1001/jama.2009.1754.
14. Iregui M, Ward S, Sherman G, Fraser VJ, Kollef MH. Clinical importance of delays in the initiation of appropriate antibiotic treatment for ventilator-associated pneumonia. Chest. 2002;122:262–8.
15. Mengelle C, Mansuy JM, Pierre A, Claudet I, Grouteau E, Micheau P, et al. The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit. J Clin Virol: Off Public Pan Am Soc Clin Virol. 2014;61:411–7. https://doi.org/10.1016/j.jcv.2014.08.023.
16. Krause JC, Panning M, Hengel H, Henneke P. The role of multiplex PCR in respiratory tract infections in children. Dtsch Arzteblatt International. 2014;111(38):639–45. https://doi.org/10.3238/arztebl.2014.0639.
17. Mayer LM, Kahlert C, Rassouli F, Vernazza P, Albrich WC. Impact of viral multiplex real-time PCR on management of respiratory tract infection: a retrospective cohort study. Pneumonia (Nathan Qld). 2017;9:4. https://doi.org/10.1186/s41479-017-0028-z.
18. Gowin E, Bartkowska-Sniatkowska A, Jonczyk-Potoczna K, Wysocka-Leszczynska J, Bobkowski W, Fichna P, et al. Assessment of the usefulness of multiplex real-time PCR tests in the diagnostic and therapeutic process of pneumonia in hospitalized children: a single-center experience. Biomed Res Int. 2017;2017:8037963. https://doi.org/10.1155/2017/8037963.
19. Baudel JL, Tankovic J, Dahoumane R, Carrat F, Galbois A, Ait-Oufella H, et al. Multiplex PCR performed of bronchoalveolar lavage fluid increases pathogen identification rate in critically ill patients with pneumonia: a pilot study. Ann Intensive Care. 2014;4:35. https://doi.org/10.1186/s13613-014-0035-7.
20. Siow WT, Koay ES, Lee CK, Lee HK, Ong V, Ngerng WJ, et al. The Use of Polymerase Chain Reaction Amplification for the Detection of Viruses and Bacteria in Severe Community-Acquired Pneumonia. Respir Int Rev Thorac Dis. 2016;92:286–94. https://doi.org/10.1159/000448555.
21. Jamal W, Al Roomi E, AbdulAziz LR, Rotimi VO. Evaluation of Curetis Unyvero, a multiplex PCR-based testing system, for rapid detection of bacteria and antibiotic resistance and impact of the assay on management of severe nosocomial pneumonia. J Clin Microbiol. 2014;52:2487–92. https://doi.org/10.1128/jcm.00325-14.
22. Schulte B, Eickmeyer H, Heininger A, Juretzek S, Karrasch M, Denis O, et al. Detection of pneumonia associated pathogens using a prototype multiplexed pneumonia test in hospitalized patients with severe pneumonia. PLoS One. 2014;9:e110566. https://doi.org/10.1371/journal.pone.0110566.
23. Kunze N, Moerer O, Steinmetz N, Schulze MH, Quintel M, Perl T. Point-of-care multiplex PCR promises short turnaround times for microbial testing in hospital-acquired pneumonia–an observational pilot study in critical ill patients. Annals Clin Microbio Antimicrob. 2015;14:33. https://doi.org/10.1186/s12941-015-0091-3.
24. Personne Y, Ozongwu C, Platt G, Basurto-Lozada P, Shamin M, Gant VA, et al. ‘Sample-in, answer-out’? Evaluation and comprehensive analysis of the Unyvero P50 pneumonia assay. Diagn Microbiol Infect Dis. 2016;86:5–10. https://doi.org/10.1016/j.diagmicrobio.2016.06.010.
25. Jorgensen JH, Pfaller MA, Carroll KC, Funke G, Landry ML, Richter SS et al. Manual of clinical microbiology, 11 Edn. Washington, DC: ASM Press; 2015.
26. Newcombe RG. Two-sided confidence intervals for the single proportion: comparison of seven methods. Stat Med. 1998;17:857–72.
27. Giuffre M, Geraci DM, Bonura C, Saporito L, Graziano G, Insinga V, et al. The increasing challenge of multidrug-resistant gram-negative Bacilli: results of a 5-year active surveillance program in a neonatal intensive care unit. Medicine. 2016;95:e3016. https://doi.org/10.1097/md.0000000000003016.
28. Logan LK, Renschler JP, Gandra S, Weinstein RA, Laxminarayan R. Carbapenem-resistant Enterobacteriaceae in Children, United States, 1999-2012. Emerg Infect Dis. 2015;21:2014–21. https://doi.org/10.3201/eid2111.150548.
29. Drew RJ, Turton JF, Hill RL, Livermore DM, Woodford N, Paulus S, et al. Emergence of carbapenem-resistant Enterobacteriaceae in a UK paediatric hospital. J Hosp Infect. 2013;84(4):300–4. https://doi.org/10.1016/j.jhin.2013.05.003.
30. De Angelis G, Restuccia G, Venturiello S, Cauda R, Malhotra-Kumar S, Goossens H, et al. Nosocomial acquisition of methicillin-resistant Staphyloccocus aureus (MRSA) and extended-spectrum beta-lactamase (ESBL) Enterobacteriaceae in hospitalised patients: a prospective multicenter study. BMC Infect Dis. 2012;12:74. https://doi.org/10.1186/1471-2334-12-74.
31. Koh J, Wong JJ, Sultana R, Wong PPC, Mok YH, Lee JH. Risk factors for mortality in children with pneumonia admitted to the pediatric intensive care unit. Pediatr Pulmonol. 2017;52:1076–84. https://doi.org/10.1002/ppul.23702.
32. Jain K, Wainwright C, Smyth AR. Bronchoscopy-guided antimicrobial therapy for cystic fibrosis. Cochrane database Syst Rev. 2016;1:Cd009530. https://doi.org/10.1002/14651858.CD009530.pub3.
33. Yazdani R, Abolhassani H, Asgardoon M, Shaghaghi M, Modaresi M, Azizi G, et al. Infectious and Noninfectious Pulmonary Complications in Patients With Primary Immunodeficiency Disorders. J Investig Allergol Clin Immunol. 2017;27:213–24. https://doi.org/10.18176/jiaci.0166.
34. Rabie H, Goussard P. Tuberculosis and pneumonia in HIV-infected children: an overview. Pneumonia (Nathan Qld). 2016;8:19. https://doi.org/10.1186/s41479-016-0021-y.
35. Stavnsbjerg C, Frimodt-Moller N, Moser C, Bjarnsholt T. Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples. BMC Infect Dis. 2017;17:233. https://doi.org/10.1186/s12879-017-2333-9.
36. Ozongwu C, Personne Y, Platt G, Jeanes C, Aydin S, Kozato N, et al. The Unyvero P55 ‘sample-in, answer-out’ pneumonia assay: a performance evaluation. Biomol Detect Quantif. 2017;. https://doi.org/10.1016/j.bdq.2017.06.001.
37. Borde JP, Hacker GA, Guschl S, Serr A, Danner T, Hubner J, et al. Diagnosis of prosthetic joint infections using UMD-Universal Kit and the automated multiplex-PCR Unyvero i60 ITI((R)) cartridge system: a pilot study. Infection. 2015;43:551–60. https://doi.org/10.1007/s15010-015-0796-4.
38. Hischebeth GT, Randau TM, Buhr JK, Wimmer MD, Hoerauf A, Molitor E, et al. Unyvero i60 implant and tissue infection (ITI) multiplex PCR system in diagnosing periprosthetic joint infection. J Microbiol Methods. 2016;121:27–32. https://doi.org/10.1016/j.mimet.2015.12.010.
39. Malandain D, Bemer P, Leroy AG, Leger J, Plouzeau C, Valentin AS, et al. Assessment of the automated multiplex-PCR Unyvero i60 ITI(R) cartridge system to diagnose prosthetic joint infection: a multicentre study. Clin Microbiol Infect: Off Public Eur Soc Clin Microbiol Infect Dis. 2017;. https://doi.org/10.1016/j.cmi.2017.05.017.
40. Unyvero Panel Overview. http://www.unyvero.com/fileadmin/Dokumente/Unyvero/Download/Broschueren/00299_Unyvero_Panel_Overview_Rev_3__ld.pdf. Accessed/12/26 2016.
41. Papan C, von Both U, Kappler M, Kammer B, Griese M, Huebner J. Pott’s disease: a major issue for an unaccompanied refugee minor. Thorax. 2017;72:282–3. https://doi.org/10.1136/thoraxjnl-2016-209468.