메뉴 건너뛰기




Volumn 4, Issue 1, 2014, Pages

Multiplex PCR performed of bronchoalveolar lavage fluid increases pathogen identification rate in critically ill patients with pneumonia: a pilot study

Author keywords

Antibiotic therapy; Bronchoalveolar lavage; Community acquired pneumonia; Hospital acquired pneumonia; Multiplex PCR; Prior antibiotic treatment; SeptiFast ; Ventilator acquired pneumonia

Indexed keywords

ANTIBIOTIC AGENT;

EID: 84914688991     PISSN: None     EISSN: 21105820     Source Type: Journal    
DOI: 10.1186/s13613-014-0035-7     Document Type: Article
Times cited : (30)

References (43)
  • 1
    • 23944434423 scopus 로고    scopus 로고
    • Current guidelines for the treatment of severe pneumonia and sepsis
    • COI: 1:CAS:528:DC%2BD2MXoslKgsLs%3D, PID: 16103664
    • Bodmann KF: Current guidelines for the treatment of severe pneumonia and sepsis.Chemotherapy 2005, 51(5):227–233.
    • (2005) Chemotherapy , vol.51 , Issue.5 , pp. 227-233
    • Bodmann, K.F.1
  • 2
    • 55849092085 scopus 로고    scopus 로고
    • Antimicrobial therapy escalation and hospital mortality among patients with health-care-associated pneumonia: a single-center experience
    • PID: 18641103
    • Zilberberg MD, Shorr AF, Micek ST, Mody SH, Kollef MH: Antimicrobial therapy escalation and hospital mortality among patients with health-care-associated pneumonia: a single-center experience.Chest 2008, 134(5):963–968.
    • (2008) Chest , vol.134 , Issue.5 , pp. 963-968
    • Zilberberg, M.D.1    Shorr, A.F.2    Micek, S.T.3    Mody, S.H.4    Kollef, M.H.5
  • 3
    • 0035088904 scopus 로고    scopus 로고
    • Impact of appropriateness of initial antibiotic therapy on the outcome of ventilator-associated pneumonia
    • COI: 1:STN:280:DC%2BD38%2FhtF2hsw%3D%3D, PID: 11396279
    • Dupont H, Mentec H, Sollet JP, Bleichner G: Impact of appropriateness of initial antibiotic therapy on the outcome of ventilator-associated pneumonia.Intensive Care Med 2001, 27(2):355–362.
    • (2001) Intensive Care Med , vol.27 , Issue.2 , pp. 355-362
    • Dupont, H.1    Mentec, H.2    Sollet, J.P.3    Bleichner, G.4
  • 4
    • 0033694879 scopus 로고    scopus 로고
    • Value of gram stain examination of lower respiratory tract secretions for early diagnosis of nosocomial pneumonia
    • COI: 1:STN:280:DC%2BD3M%2FkvFyqsQ%3D%3D, PID: 11069804
    • Blot F, Raynard B, Chachaty E, Tancrede C, Antoun S, Nitenberg G: Value of gram stain examination of lower respiratory tract secretions for early diagnosis of nosocomial pneumonia.Am J Respir Crit Care Med 2000, 162(5):1731–1737.
    • (2000) Am J Respir Crit Care Med , vol.162 , Issue.5 , pp. 1731-1737
    • Blot, F.1    Raynard, B.2    Chachaty, E.3    Tancrede, C.4    Antoun, S.5    Nitenberg, G.6
  • 5
    • 0025760390 scopus 로고
    • Diagnosis of nosocomial pneumonia in mechanically ventilated patients. Comparison of a plugged telescoping catheter with the protected specimen brush
    • COI: 1:STN:280:DyaK3M3itFeltw%3D%3D, PID: 2024814
    • Pham LH, Brun-Buisson C, Legrand P, Rauss A, Verra F, Brochard L, Lemaire F: Diagnosis of nosocomial pneumonia in mechanically ventilated patients. Comparison of a plugged telescoping catheter with the protected specimen brush.Am Rev Respir Dis 1991, 143(5 Pt 1):1055–1061.
    • (1991) Am Rev Respir Dis , vol.143 , Issue.5 , pp. 1055-1061
    • Pham, L.H.1    Brun-Buisson, C.2    Legrand, P.3    Rauss, A.4    Verra, F.5    Brochard, L.6    Lemaire, F.7
  • 6
    • 77955689254 scopus 로고    scopus 로고
    • Diagnostic techniques and procedures for establishing the microbial etiology of ventilator-associated pneumonia for clinical trials: the pros for quantitative cultures
    • PID: 20597677
    • Chastre J, Trouillet JL, Combes A, Luyt CE: Diagnostic techniques and procedures for establishing the microbial etiology of ventilator-associated pneumonia for clinical trials: the pros for quantitative cultures.Clin Infect Dis 2010, 51(Suppl 1):S88–S92.
    • (2010) Clin Infect Dis , vol.51 , pp. S88-S92
    • Chastre, J.1    Trouillet, J.L.2    Combes, A.3    Luyt, C.E.4
  • 10
    • 42949089382 scopus 로고    scopus 로고
    • Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia
    • COI: 1:CAS:528:DC%2BD1cXltlSgtr4%3D, PID: 18434904
    • Louie RF, Tang Z, Albertson TE, Cohen S, Tran NK, Kost GJ: Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia.Crit Care Med 2008, 36(5):1487–1492.
    • (2008) Crit Care Med , vol.36 , Issue.5 , pp. 1487-1492
    • Louie, R.F.1    Tang, Z.2    Albertson, T.E.3    Cohen, S.4    Tran, N.K.5    Kost, G.J.6
  • 14
  • 15
    • 80054723483 scopus 로고    scopus 로고
    • Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections
    • COI: 1:STN:280:DC%2BC3MjmtlKitw%3D%3D, PID: 21373774
    • Josefson P, Stralin K, Ohlin A, Ennefors T, Dragsten B, Andersson L, Fredlung H, Mölling P, Olcen P: Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections.Eur J Clin Microbiol Infect Dis 2011, 30(9):1127–1134.
    • (2011) Eur J Clin Microbiol Infect Dis , vol.30 , Issue.9 , pp. 1127-1134
    • Josefson, P.1    Stralin, K.2    Ohlin, A.3    Ennefors, T.4    Dragsten, B.5    Andersson, L.6    Fredlung, H.7    Mölling, P.8    Olcen, P.9
  • 17
    • 78649233811 scopus 로고    scopus 로고
    • Evaluation of a multiplex real-time PCR assay for detecting pathogens in cardiac valve tissue in patients with endocarditis
    • PID: 20875362
    • Fernandez AL, Varela E, Martinez L, Martinez A, Sierra J, Gonzalez-Juanatey JR, Regueiro B: Evaluation of a multiplex real-time PCR assay for detecting pathogens in cardiac valve tissue in patients with endocarditis.Rev Esp Cardiol 2010, 63(10):1205–1208.
    • (2010) Rev Esp Cardiol , vol.63 , Issue.10 , pp. 1205-1208
    • Fernandez, A.L.1    Varela, E.2    Martinez, L.3    Martinez, A.4    Sierra, J.5    Gonzalez-Juanatey, J.R.6    Regueiro, B.7
  • 18
    • 22144444944 scopus 로고    scopus 로고
    • The international sepsis forum consensus conference on definitions of infection in the intensive care unit
    • PID: 16003060
    • Calandra T, Cohen J: The international sepsis forum consensus conference on definitions of infection in the intensive care unit.Crit Care Med 2005, 33(7):1538–1548.
    • (2005) Crit Care Med , vol.33 , Issue.7 , pp. 1538-1548
    • Calandra, T.1    Cohen, J.2
  • 19
    • 0032851762 scopus 로고    scopus 로고
    • Clinical diagnosis of ventilator associated pneumonia revisited: comparative validation using immediate post-mortem lung biopsies
    • COI: 1:STN:280:DyaK1MvitlOnug%3D%3D, PID: 10491448
    • Fabregas N, Ewig S, Torres A, El-Ebiary M, Ramirez J, de La Bellacasa JP, Bauer T, Cabello H: Clinical diagnosis of ventilator associated pneumonia revisited: comparative validation using immediate post-mortem lung biopsies.Thorax 1999, 54(10):867–873.
    • (1999) Thorax , vol.54 , Issue.10 , pp. 867-873
    • Fabregas, N.1    Ewig, S.2    Torres, A.3    El-Ebiary, M.4    Ramirez, J.5    de La Bellacasa, J.P.6    Bauer, T.7    Cabello, H.8
  • 20
    • 13644269309 scopus 로고    scopus 로고
    • Guidelines for the management of adults with hospital-acquired, ventilator-associated, and healthcare-associated pneumonia
    • Documents ATS: Guidelines for the management of adults with hospital-acquired, ventilator-associated, and healthcare-associated pneumonia.Am J Respir Crit Care Med 2005, 171(4):388–416.
    • (2005) Am J Respir Crit Care Med , vol.171 , Issue.4 , pp. 388-416
    • Documents, A.T.S.1
  • 22
    • 0028036903 scopus 로고
    • Bronchoscopic diagnosis of pneumonia
    • COI: 1:STN:280:DyaK2M7js1ajtQ%3D%3D, PID: 7834604
    • Baselski VS, Wunderink RG: Bronchoscopic diagnosis of pneumonia.Clin Microbiol Rev 1994, 7(4):533–558.
    • (1994) Clin Microbiol Rev , vol.7 , Issue.4 , pp. 533-558
    • Baselski, V.S.1    Wunderink, R.G.2
  • 23
    • 77954572217 scopus 로고    scopus 로고
    • Long-term stability of circulating nucleosomes in serum
    • COI: 1:CAS:528:DC%2BC3cXhtVWmu7zN, PID: 20592350
    • Holdenrieder S, Von Pawel J, Nagel D, Stieber P: Long-term stability of circulating nucleosomes in serum.Anticancer Res 2010, 30(5):1613–1615.
    • (2010) Anticancer Res , vol.30 , Issue.5 , pp. 1613-1615
    • Holdenrieder, S.1    Von Pawel, J.2    Nagel, D.3    Stieber, P.4
  • 25
    • 78651257740 scopus 로고    scopus 로고
    • Inappropriate antibiotic therapy in gram-negative sepsis increases hospital length of stay
    • COI: 1:CAS:528:DC%2BC3cXhs1Wjtb7I, PID: 20890186
    • Shorr AF, Micek ST, Welch EC, Doherty JA, Reichley RM, Kollef MH: Inappropriate antibiotic therapy in gram-negative sepsis increases hospital length of stay.Crit Care Med 2011, 39(1):46–51.
    • (2011) Crit Care Med , vol.39 , Issue.1 , pp. 46-51
    • Shorr, A.F.1    Micek, S.T.2    Welch, E.C.3    Doherty, J.A.4    Reichley, R.M.5    Kollef, M.H.6
  • 26
    • 79951933611 scopus 로고    scopus 로고
    • Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR
    • COI: 1:CAS:528:DC%2BC3MXislCgsrk%3D, PID: 21359187
    • Lehmann LE, Hauser S, Malinka T, Klaschik S, Weber SU, Schewe JC, Stüber F, Book M: Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.PLoS One 2011, 6(2):e17146.
    • (2011) PLoS One , vol.6 , Issue.2 , pp. e17146
    • Lehmann, L.E.1    Hauser, S.2    Malinka, T.3    Klaschik, S.4    Weber, S.U.5    Schewe, J.C.6    Stüber, F.7    Book, M.8
  • 27
    • 79960957317 scopus 로고    scopus 로고
    • Performance of the LightCycler SeptiFast test mgrade in detecting microbial pathogens in purulent fluids
    • PID: 21715593
    • Sancho-Tello S, Bravo D, Borras R, Costa E, Munoz-Cobo B, Navarro D: Performance of the LightCycler SeptiFast test mgrade in detecting microbial pathogens in purulent fluids.J Clin Microbiol 2011, 49(8):2988–2991.
    • (2011) J Clin Microbiol , vol.49 , Issue.8 , pp. 2988-2991
    • Sancho-Tello, S.1    Bravo, D.2    Borras, R.3    Costa, E.4    Munoz-Cobo, B.5    Navarro, D.6
  • 29
    • 70350048877 scopus 로고    scopus 로고
    • Microbiological sepsis screening in surgical ICU patients with the “lightCycler” septifast test–a pilot study
    • COI: 1:STN:280:DC%2BD1Mvos1CjtA%3D%3D, PID: 19536720
    • Lodes U, Meyer F, Konig B, Lippert H: Microbiological sepsis screening in surgical ICU patients with the “lightCycler” septifast test–a pilot study.Zentralbl Chir 2009, 134(3):249–253.
    • (2009) Zentralbl Chir , vol.134 , Issue.3 , pp. 249-253
    • Lodes, U.1    Meyer, F.2    Konig, B.3    Lippert, H.4
  • 30
    • 77953920184 scopus 로고    scopus 로고
    • Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood
    • COI: 1:CAS:528:DC%2BC3cXot1Gmsb8%3D, PID: 19689465
    • Wallet F, Nseir S, Baumann L, Herwegh S, Sendid B, Boulo M, Roussel-Delvallez M, Durocher AV, Courcol RJ: Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood.Clin Microbiol Infect 2010, 16(6):774–779.
    • (2010) Clin Microbiol Infect , vol.16 , Issue.6 , pp. 774-779
    • Wallet, F.1    Nseir, S.2    Baumann, L.3    Herwegh, S.4    Sendid, B.5    Boulo, M.6    Roussel-Delvallez, M.7    Durocher, A.V.8    Courcol, R.J.9
  • 31
    • 77957815662 scopus 로고    scopus 로고
    • Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia
    • COI: 1:STN:280:DC%2BC3cfmsFGhtA%3D%3D, PID: 20720024
    • Lamoth F, Jaton K, Prod’hom G, Senn L, Bille J, Calandra T, Marchetti O: Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia.J Clin Microbiol 2010, 48(10):3510–3516.
    • (2010) J Clin Microbiol , vol.48 , Issue.10 , pp. 3510-3516
    • Lamoth, F.1    Jaton, K.2    Prod’hom, G.3    Senn, L.4    Bille, J.5    Calandra, T.6    Marchetti, O.7
  • 32
    • 0038555339 scopus 로고    scopus 로고
    • Community-acquired bloodstream infection in critically ill adult patients: impact of shock and inappropriate antibiotic therapy on survival
    • PID: 12740282
    • Valles J, Rello J, Ochagavia A, Garnacho J, Alcala MA: Community-acquired bloodstream infection in critically ill adult patients: impact of shock and inappropriate antibiotic therapy on survival.Chest 2003, 123(5):1615–1624.
    • (2003) Chest , vol.123 , Issue.5 , pp. 1615-1624
    • Valles, J.1    Rello, J.2    Ochagavia, A.3    Garnacho, J.4    Alcala, M.A.5
  • 33
    • 79957533179 scopus 로고    scopus 로고
    • Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis
    • PID: 21471340
    • Lucignano B, Ranno S, Liesenfeld O, Pizzorno B, Putignani L, Bernaschi P, Menichella D: Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis.J Clin Microbiol 2011, 49(6):2252–2258.
    • (2011) J Clin Microbiol , vol.49 , Issue.6 , pp. 2252-2258
    • Lucignano, B.1    Ranno, S.2    Liesenfeld, O.3    Pizzorno, B.4    Putignani, L.5    Bernaschi, P.6    Menichella, D.7
  • 34
    • 53749097248 scopus 로고    scopus 로고
    • LightCycler SeptiFast assay as a tool for the rapid diagnosis of sepsis in patients during antimicrobial therapy
    • PID: 18809565
    • Vince A, Lepej SZ, Barsic B, Dusek D, Mitrovic Z, Serventi-Seiwerth R, Labar B: LightCycler SeptiFast assay as a tool for the rapid diagnosis of sepsis in patients during antimicrobial therapy.J Med Microbiol 2008, 57(Pt 10):1306–1307.
    • (2008) J Med Microbiol , vol.57 , pp. 1306-1307
    • Vince, A.1    Lepej, S.Z.2    Barsic, B.3    Dusek, D.4    Mitrovic, Z.5    Serventi-Seiwerth, R.6    Labar, B.7
  • 35
    • 53649095457 scopus 로고    scopus 로고
    • Evaluation of the xpert methicillin-resistant staphylococcus aureus (MRSA) assay using the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens
    • COI: 1:CAS:528:DC%2BD1cXht12rurfL, PID: 18685003
    • Rossney AS, Herra CM, Brennan GI, Morgan PM, O’Connell B: Evaluation of the xpert methicillin-resistant staphylococcus aureus (MRSA) assay using the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens.J Clin Microbiol 2008, 46(10):3285–3290.
    • (2008) J Clin Microbiol , vol.46 , Issue.10 , pp. 3285-3290
    • Rossney, A.S.1    Herra, C.M.2    Brennan, G.I.3    Morgan, P.M.4    O’Connell, B.5
  • 36
    • 79952324639 scopus 로고    scopus 로고
    • Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method
    • PID: 21191053
    • Moure R, Munoz L, Torres M, Santin M, Martin R, Alcaide F: Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method.J Clin Microbiol 2011, 49(3):1137–1139.
    • (2011) J Clin Microbiol , vol.49 , Issue.3 , pp. 1137-1139
    • Moure, R.1    Munoz, L.2    Torres, M.3    Santin, M.4    Martin, R.5    Alcaide, F.6
  • 37
    • 66149115670 scopus 로고    scopus 로고
    • Identification of methicillin-resistant or methicillin-susceptible Staphylococcus aureus in blood cultures and wound swabs by GeneXpert
    • COI: 1:STN:280:DC%2BD1MzgvV2qtw%3D%3D, PID: 19261790
    • Parta M, Goebel M, Matloobi M, Stager C, Musher DM: Identification of methicillin-resistant or methicillin-susceptibleStaphylococcus aureusin blood cultures and wound swabs by GeneXpert.J Clin Microbiol 2009, 47(5):1609–1610.
    • (2009) J Clin Microbiol , vol.47 , Issue.5 , pp. 1609-1610
    • Parta, M.1    Goebel, M.2    Matloobi, M.3    Stager, C.4    Musher, D.M.5
  • 38
    • 79953904602 scopus 로고    scopus 로고
    • Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system
    • COI: 1:CAS:528:DC%2BC3MXhtFKrt7zL, PID: 21270230
    • Hillemann D, Rusch-Gerdes S, Boehme C, Richter E: Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system.J Clin Microbiol 2011, 49(4):1202–1205.
    • (2011) J Clin Microbiol , vol.49 , Issue.4 , pp. 1202-1205
    • Hillemann, D.1    Rusch-Gerdes, S.2    Boehme, C.3    Richter, E.4
  • 39
    • 79960965903 scopus 로고    scopus 로고
    • Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification
    • PID: 21632894
    • Neville SA, Lecordier A, Ziochos H, Chater MJ, Gosbell IB, Maley MW, van Hal SJ: Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification.J Clin Microbiol 2011, 49(8):2980–2984.
    • (2011) J Clin Microbiol , vol.49 , Issue.8 , pp. 2980-2984
    • Neville, S.A.1    Lecordier, A.2    Ziochos, H.3    Chater, M.J.4    Gosbell, I.B.5    Maley, M.W.6    van Hal, S.J.7
  • 40
    • 79953834934 scopus 로고    scopus 로고
    • Intact cell MALDI-TOF mass spectrometry-based approaches for the diagnosis of bloodstream infections
    • COI: 1:CAS:528:DC%2BC3MXktl2isr0%3D, PID: 21463238
    • La Scola B: Intact cell MALDI-TOF mass spectrometry-based approaches for the diagnosis of bloodstream infections.Expert Rev Mol Diagn 2011, 11(3):287–298.
    • (2011) Expert Rev Mol Diagn , vol.11 , Issue.3 , pp. 287-298
    • La Scola, B.1
  • 42
    • 79961066587 scopus 로고    scopus 로고
    • Usefulness of real-time PCR for the diagnosis of sepsis in ICU-acquired infections
    • COI: 1:CAS:528:DC%2BC3MXhtVSmsLzL, PID: 21679147
    • Wallet F, Loiez C, Herwegh S, Courcol RJ: Usefulness of real-time PCR for the diagnosis of sepsis in ICU-acquired infections.Infect Disord Drug Targets 2011, 11(4):348–353.
    • (2011) Infect Disord Drug Targets , vol.11 , Issue.4 , pp. 348-353
    • Wallet, F.1    Loiez, C.2    Herwegh, S.3    Courcol, R.J.4
  • 43
    • 84871867383 scopus 로고    scopus 로고
    • Cost analysis of real-time polymerase chain reaction microbiological diagnosis in patients with septic shock
    • COI: 1:STN:280:DC%2BC3s7nsVKjtQ%3D%3D, PID: 23194204
    • Alvarez J, Mar J, Varela-Ledo E, Garea M, Matinez-Lamas L, Rodriguez J, Regueiro B: Cost analysis of real-time polymerase chain reaction microbiological diagnosis in patients with septic shock.Anaesth Intensive Care 2012, 40(6):958–963.
    • (2012) Anaesth Intensive Care , vol.40 , Issue.6 , pp. 958-963
    • Alvarez, J.1    Mar, J.2    Varela-Ledo, E.3    Garea, M.4    Matinez-Lamas, L.5    Rodriguez, J.6    Regueiro, B.7


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.