The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit

Journal of Clinical Virology

Volumn 61, Issue 3, 2014, Pages 411-417

  Mengelle, C ^a   Mansuy, J M ^a   Pierre, A ^a   Claudet, I ^a   Grouteau, E ^a   Micheau, P ^a   Saune K ^a,b   Izopet, J ^a,b  

^a TOULOUSE UNIVERSITY HOSPITAL   (France)

^b INSERM   (France)

Author keywords

Children; Multiplex PCR; Respiratory; Spread; Symptoms; Viruses

Indexed keywords

ADENOVIRUS; ARTICLE; CHILD; CONTROLLED STUDY; CORONAVIRUS; EMERGENCY WARD; FEMALE; HUMAN; INFANT; INFLUENZA VIRUS; LOWER RESPIRATORY TRACT INFECTION; MAJOR CLINICAL STUDY; MALE; METAPNEUMOVIRUS; MULTIPLEX POLYMERASE CHAIN REACTION; PARAINFLUENZA VIRUS; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY TRACT INFECTION; RHINOVIRUS; SYMPTOM; THROAT CULTURE; UPPER RESPIRATORY TRACT INFECTION; VIRAL RESPIRATORY TRACT INFECTION; VIRUS DETECTION; VIRUS DIAGNOSIS; VIRUS TRANSMISSION; WINTER; ADOLESCENT; CLASSIFICATION; EMERGENCY HEALTH SERVICE; EMERGENCY MEDICINE; EVALUATION STUDY; ISOLATION AND PURIFICATION; MOLECULAR DIAGNOSIS; NEWBORN; PRESCHOOL CHILD; PROCEDURES; RESPIRATORY TRACT INFECTIONS; VIRUS; VIRUS DISEASES;

ADOLESCENT; CHILD; CHILD, PRESCHOOL; EMERGENCY MEDICINE; EMERGENCY SERVICE, HOSPITAL; HUMANS; INFANT; INFANT, NEWBORN; MALE; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; RESPIRATORY TRACT INFECTIONS; VIRUS DISEASES; VIRUSES;

EID: 84908551308     PISSN: 13866532     EISSN: 18735967     Source Type: Journal    
DOI: 10.1016/j.jcv.2014.08.023     Document Type: Article

References (25)

1. Nicholson K.G., McNally T., Silverman M., Simons P., Stockton J.D., Zambon M.C. Rates of hospitalisation for influenza, respiratory syncytial virus and human metapneumovirus among infants and young children. Vaccine 2006, 24:102-108.
2. Massin M.M., Montesanti J., Gerard P., Lepage P. Spectrum and frequency of illness presenting to a pediatric emergency department. Acta Clin Belg 2006, 61:161-165.
3. Schouten J.P., McElgunn C.J., Waaijer R., Zwijnenburg D., Diepvens F., Pals G. Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification. Nucleic Acids Res 2002, 30:e57.
4. Mansuy J.M., Mengelle C., Da Silva I., Grog I., Saune K., Izopet J. Performance of a rapid molecular multiplex assay for the detection of influenza and picornaviruses. Scand J Infect Dis 2012, 44:963-968.
5. Simoes E.A., Patel C., Sung W.K., Lee C.W., Loh K.H., Lucero M., et al. Pathogen chip for respiratory tract infections. J Clin Microbiol 2013, 51:945-953.
6. Bhat N., Tokarz R., Jain K., Haq S., Weatherholtz R., Chandran A., et al. A Prospective Study of Agents associated with acute respiratory infection among young American Indian children. Pediatr Infect Dis J 2013, 32:e324-e333.
7. Freymuth F., Vabret A., Cuvillon-Nimal D., Simon S., Dina J., Legrand L., et al. Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness. J Med Virol 2006, 78:1498-1504.
8. Ruohola A., Waris M., Allander T., Ziegler T., Heikkinen T., Ruuskanen O. Viral etiology of common cold in children, Finland. Emerg Infect Dis 2009, 15:344-346.
9. Brittain-Long R., Westin J., Olofsson S., Lindh M., Andersson L.M. Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-duration of symptoms significantly affects detection rate. J Clin Virol 2010, 47:263-267.
10. Papadopoulos N.G. Do rhinoviruses cause pneumonia in children?. Paediatr Respir Rev 2004, 5:S191-S195.
11. O'Callaghan-Gordo C., Bassat Q., Diez-Padrisa N., Morais L., Machevo S., Nhampossa T., et al. Lower respiratory tract infections associated with rhinovirus during infancy and increased risk of wheezing during childhood. A cohort study. PLoS ONE 2013, 8:e69370.
12. Stempel H.E., Martin E.T., Kuypers J., Englund J.A., Zerr D.M. Multiple viral respiratory pathogens in children with bronchiolitis. Acta Paediatr 2009, 98:123-126.
13. Ruuskanen O., Lahti E., Jennings L.C., Murdoch D.R. Viral pneumonia. Lancet 2011, 377:1264-1275.
14. Murtagh P., Giubergia V., Viale D., Bauer G., Pena H.G. Lower respiratory infections by adenovirus in children. Clinical features and risk factors for bronchiolitis obliterans and mortality. Pediatr Pulmonol 2009, 44:450-456.
15. Jean A., Quach C., Yung A., Semret M. Severity and outcome associated with human coronavirus OC43 infections among children. Pediatr Infect Dis J 2013, 32:325-329.
16. Edwards K.M., Zhu Y., Griffin M.R., Weinberg G.A., Hall C.B., Szilagyi P.G., et al. Burden of human metapneumovirus infection in young children. N Engl J Med 2013, 368:633-643.
17. Cilla G., Onate E., Perez-Yarza E.G., Montes M., Vicente D., Perez-Trallero E. Viruses in community-acquired pneumonia in children aged less than 3 years old: high rate of viral coinfection. J Med Virol 2008, 80:1843-1849.
18. Dao C.N., Kamimoto L., Nowell M., Reingold A., Gershman K., Meek J., et al. Adult hospitalizations for laboratory-positive influenza during the 2005-2006 through 2007-2008 seasons in the United States. J Infect Dis 2010, 202:881-888.
19. Pabbaraju K., Tokaryk K.L., Wong S., Fox J.D. Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections. J Clin Microbiol 2008, 46:3056-3062.
20. Fairchok M.P., Martin E.T., Chambers S., Kuypers J., Behrens M., Braun L.E., et al. Epidemiology of viral respiratory tract infections in a prospective cohort of infants and toddlers attending daycare. J Clin Virol 2010, 49:16-20.
21. Martin E.T., Kuypers J., Wald A., Englund J.A. Multiple versus single virus respiratory infections: viral load and clinical disease severity in hospitalized children. Influenza Other Respir Viruses 2012, 6:71-77.
22. da Silva E.R., Pitrez P.M., Arruda E., Mattiello R., Sarria E.E., de Paula F.E., et al. Severe lower respiratory tract infection in infants and toddlers from a non-affluent population: viral etiology and co-detection as risk factors. BMC Infect Dis 2013, 13:41.
23. Semple M.G., Cowell A., Dove W., Greensill J., McNamara P.S., Halfhide C., et al. Dual infection of infants by human metapneumovirus and human respiratory syncytial virus is strongly associated with severe bronchiolitis. J Infect Dis 2005, 191:382-386.
24. Aberle J.H., Aberle S.W., Pracher E., Hutter H.P., Kundi M., Popow-Kraupp T. Single versus dual respiratory virus infections in hospitalized infants: impact on clinical course of disease and interferon-gamma response. Pediatr Infect Dis J 2005, 24:605-610.
25. Grondahl B., Ankermann T., von Bismarck P., Rockahr S., Kowalzik F., Gehring S., et al. The 2009 pandemic influenza A(H1N1) coincides with changes in the epidemiology of other viral pathogens causing acute respiratory tract infections in children. Infection 2013, 42:303-308.