Assessment of the usefulness of multiplex real-time PCR tests in the diagnostic and therapeutic process of pneumonia in hospitalized Children: A Single-Center Experience

BioMed Research International

Volumn 2017, Issue , 2017, Pages

  Gowin, Ewelina ^a   Bartkowska Śniatkowska, Alicja ^a   Jończyk Potoczna, Katarzyna ^a   Wysocka Leszczyńska, Joanna ^a   Bobkowski, Waldemar ^a   Fichna, Piotr ^a   Sobkowiak, Paulina ^a   Mazur Melewska, Katarzyna ^a   Brȩborowicz, Anna ^a   Wysocki, Jacek ^a   Januszkiewicz Lewandowska, Danuta ^a,b  

^a POZNAN UNIVERSITY OF MEDICAL SCIENCES   (Poland)

^b INSTITUTE OF HUMAN GENETICS   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

COTRIMOXAZOLE; MACROLIDE; OSELTAMIVIR; BACTERIAL DNA; VIRUS DNA;

AIRWAY OBSTRUCTION; ANTIBIOTIC THERAPY; ARTICLE; BACTERIAL PNEUMONIA; BACTERIAL STRAIN; BACTERIUM DETECTION; CHILD; CLINICAL ASSESSMENT; COMORBIDITY; CONTROLLED STUDY; DIAGNOSTIC TEST; DIAGNOSTIC TEST ACCURACY STUDY; FEMALE; GROUPS BY AGE; HOSPITALIZATION; HOSPITALIZED CHILD; HUMAN; INFLUENZA; LEGIONNAIRE DISEASE; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; PNEUMOCYSTIS; PNEUMOCYSTIS PNEUMONIA; PNEUMONIA; PREDICTIVE VALUE; PRESCHOOL CHILD; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYSTEM; RESPIRATORY TRACT INFECTION; RISK FACTOR; TEACHING HOSPITAL; VIRAL RESPIRATORY TRACT INFECTION; VIRUS DETECTION; VIRUS PNEUMONIA; BACTERIUM; BLOOD; CLASSIFICATION; GENETICS; INFANT; ISOLATION AND PURIFICATION; MICROBIOLOGY; PATHOGENICITY; VIROLOGY; VIRUS;

BACTERIA; CHILD; CHILD, PRESCHOOL; DNA, BACTERIAL; DNA, VIRAL; FEMALE; HUMANS; INFANT; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; PNEUMONIA; REAL-TIME POLYMERASE CHAIN REACTION; VIRUSES;

EID: 85011875184     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2017/8037963     Document Type: Article

References (25)

1. B. G. Williams, E. Gouws, C. Boschi-Pinto, J. Bryce, and C. Dye, "Estimates of world-wide distribution of child deaths from acute respiratory infections, " Lancet Infectious Diseases, vol. 2, no. 1, pp. 25-32, 2002.
2. World Health Organization Acute respiratory infections in children 2012 http://www. who. int/vaccine research/diseases/ ari/en/index. html.
3. C. Prat, J. Domínguez, C. Rodrigo et al., "Procalcitonin, Creactive protein and leukocyte count in children with lower respiratory tract infection, " The Pediatric Infectious Disease Journal, vol. 22, no. 11, pp. 963-967, 2003.
4. M. Harris, J. Clark, N. Coote et al., "British Thoracic Society guidelines for the management of community acquired pneumonia in children: update 2011, " Thorax, vol. 66, no. 2, pp. ii1-ii23, 2011.
5. J. S. Bradley, C. L. Byington, S. S. Shah et al., "The management of community-acquired pneumonia in infants and children older than 3 months of age: clinical practice guidelines by the Pediatric Infectious Diseases Society and the Infectious Diseases Society of America, " Clinical Infectious Diseases, vol. 53, no. 7, pp. e25-e76, 2011.
6. W. Hryniewicz, T. Ozorowski, and A. Radzikowski, "Rekomendacje postȩpowania w pozaszpitalnych zakazeniach układu oddechowego, " 2010, http://www. antybiotyki. edu. pl/.
7. O. Ruuskanen, E. Lahti, L. C. Jennings, and D. R. Murdoch, "Viral pneumonia, "TheLancet, vol. 377, no. 9773, pp. 1264-1275, 2011.
8. S. Esposito, C. Daleno, G. Prunotto et al., "Impact of viral infections in children with community-acquired pneumonia: results of a study of 17 respiratory viruses, " Influenza and other Respiratory Viruses, vol. 7, no. 1, pp. 18-26, 2013.
9. S. Esposito, C. G. Molteni, C. Daleno et al., "Clinical and socioeconomic impact of different types and subtypes of seasonal influenza viruses in children during influenza seasons 2007/2008 and 2008/2009, " BMC Infectious Diseases, vol. 11, article 271, 2011.
10. J. Mahony, S. Chong, F. Merante et al., "Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay, " Journal of Clinical Microbiology, vol. 45, no. 9, pp. 2965-2970, 2007.
11. A. M. Caliendo, "Multiplex PCR and emerging technologies for the detection of respiratory pathogens, " Clinical Infectious Diseases, vol. 52, no. 4, pp. S326-S330, 2011.
12. S. Bierbaum, N. Königsfeld, N. Besazza et al., "Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study), " European Journal of Clinical Microbiology and Infectious Diseases, vol. 31, no. 10, pp. 2851-2861, 2012.
13. J. O. Wishaupt, A. Russcher, L. C. Smeets, F. G. A. Versteegh, and N. G. Hartwig, "Clinical impact of RT-PCR for pediatric acute respiratory infections: a controlled clinical trial, " Pediatrics, vol. 128, no. 5, pp. e1113-e1120, 2011.
14. J. A. I. Weigl, W. Puppe, B. Gröndahl, and H.-J. Schmitt, "Epidemiological investigation of nine respiratory pathogens in hospitalized children in Germany using multiplex reversetranscriptase polymerase chain reaction, " European Journal of Clinical Microbiology and Infectious Diseases, vol. 19, no. 5, pp. 336-343, 2000.
15. C. Mengelle, J. M. Mansuy, A. Pierre et al., "The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit, " Journal of Clinical Virology, vol. 61, no. 3, pp. 411-417, 2014.
16. M. Cevey-Macherel, A. Galetto-Lacour, A. Gervaix et al., "Etiology of community-acquired pneumonia in hospitalized children based on WHO clinical guidelines, " European Journal of Pediatrics, vol. 168, no. 12, pp. 1429-1436, 2009.
17. M. N. Tsolia, S. Psarras, A. Bossios et al., "Etiology of community-acquired pneumonia in hospitalized school-age children: evidence for high prevalence of viral infections, " Clinical Infectious Diseases, vol. 39, no. 5, pp. 681-686, 2004.
18. K. L. Anders, H. L. Nguyen, N. M. Nguyen et al., "Epidemiology and virology of acute respiratory infections during the first year of life: a birth cohort study in Vietnam, " Pediatric Infectious Disease Journal, vol. 34, no. 4, pp. 361-370, 2015.
19. E. T. Martin, J. Kuypers, A. Wald, and J. A. Englund, "Multiple versus single virus respiratory infections: viral load and clinical disease severity in hospitalized children, " Influenza and other Respiratory Viruses, vol. 6, no. 1, pp. 71-77, 2012.
20. J. Englund, T. Feuchtinger, and P. Ljungman, "Viral infections in immunocompromised patients, " Biology of Blood and Marrow Transplantation, vol. 17, no. 1, supplement, pp. S2-S5, 2011.
21. L. Bonzel, T. Tenenbaum, H. Schroten, O. Schildgen, S. Schweitzer-Krantz, and O. Adams, "Frequent detection of viral coinfection in children hospitalized with acute respiratory tract infection using a real-time polymerase chain reaction, " The Pediatric Infectious Disease Journal, vol. 27, no. 7, pp. 589-594, 2008.
22. S. Esposito, R. Cohen, J. D. Domingo et al., "Antibiotic therapy for pediatric community-acquired pneumonia: do we know when, what and for how long to treat?" Pediatric Infectious Disease Journal, vol. 31, no. 6, pp. e78-e85, 2012.
23. A. Mejias and O. Ramilo, "New options in the treatment of respiratory syncytial virus disease, " Journal of Infection, vol. 71, no. 1, pp. S80-S87, 2015.
24. A. Waghmare, T. Wagner, R. Andrews et al., "Successful treatment of parainfluenza virus respiratory tract infection with DAS181 in 4 immunocompromised children, " Journal of the Pediatric Infectious Diseases Society, vol. 4, no. 2, pp. 114-118, 2015.
25. E. De Clercq, "Chemotherapy of respiratory syncytial virus infections: the final breakthrough, " International Journal of Antimicrobial Agents, vol. 45, no. 3, pp. 234-237, 2015.