Chromatin and Single-Cell RNA-Seq Profiling Reveal Dynamic Signaling and Metabolic Transitions during Human Spermatogonial Stem Cell Development

Cell Stem Cell

Volumn 21, Issue 4, 2017, Pages 533-546.e6

  Guo, Jingtao ^a   Grow, Edward J ^a   Yi, Chongil ^a   Mlcochova, Hana ^b   Maher, Geoffrey J ^b   Lindskog, Cecilia ^c   Murphy, Patrick J ^a   Wike, Candice L ^a   Carrell, Douglas T ^d   Goriely, Anne ^b   Hotaling, James M ^d   Cairns, Bradley R ^a  

^a UNIVERSITY OF UTAH   (United States)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UPPSALA UNIVERSITY   (Sweden)

^d UNIVERSITY OF UTAH HEALTH SCIENCES CENTER   (United States)

Author keywords

DNA methylation; hormone receptors; human spermatogonial stem cells; metabolism; open chromatin; pluripotency; single cell RNA seq; spermatogenesis

Indexed keywords

DNA; GLUCOSE; HORMONE RECEPTOR; OCTAMER TRANSCRIPTION FACTOR 4; RNA; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR DMRT1; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR NFY SUBUNIT ALPHA; TRANSCRIPTION FACTOR NFY SUBUNIT BETA; TRANSCRIPTOME; UNCLASSIFIED DRUG; CELL SURFACE RECEPTOR; STAGE SPECIFIC EMBRYO ANTIGEN; STAGE-SPECIFIC EMBRYONIC ANTIGEN-4;

ARTICLE; CELL CYCLE; CELL DIFFERENTIATION; CELL MATURATION; CELL PROLIFERATION; CHROMATIN; CONTROLLED STUDY; DEVELOPMENTAL STAGE; DNA METHYLATION; EMBRYO; EMBRYO DEVELOPMENT; EPIGENETICS; GENE CLUSTER; GENE REPRESSION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; METABOLISM; MITOCHONDRION; MOLECULAR DYNAMICS; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; RNA SEQUENCE; SIGNAL TRANSDUCTION; SINGLE CELL ANALYSIS; SPERMATOGONIUM; TRANSCRIPTION REGULATION; TRANSCRIPTOMICS; BINDING SITE; CLUSTER ANALYSIS; CYTOLOGY; GENETIC TRANSCRIPTION; GENETICS; GENOMICS; MALE; MEIOSIS; NUCLEOTIDE REPEAT; NUCLEOTIDE SEQUENCE; PROCEDURES; REPRODUCIBILITY; SEMINIFEROUS TUBULE; SEQUENCE ANALYSIS; STEM CELL;

BASE SEQUENCE; BINDING SITES; CHROMATIN; CLUSTER ANALYSIS; DNA; DNA METHYLATION; GENOMICS; HUMANS; MALE; MEIOSIS; PLURIPOTENT STEM CELLS; RECEPTORS, CELL SURFACE; REPETITIVE SEQUENCES, NUCLEIC ACID; REPRODUCIBILITY OF RESULTS; SEMINIFEROUS TUBULES; SEQUENCE ANALYSIS, RNA; SIGNAL TRANSDUCTION; SINGLE-CELL ANALYSIS; SPERMATOGONIA; STAGE-SPECIFIC EMBRYONIC ANTIGENS; STEM CELLS; TRANSCRIPTION, GENETIC; TRANSCRIPTOME;

EID: 85032388182     PISSN: 19345909     EISSN: 18759777     Source Type: Journal    
DOI: 10.1016/j.stem.2017.09.003     Document Type: Article

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