Psychrophilic proteases dramatically reduce single-cell RNA-seq artifacts: A molecular atlas of kidney development

Development (Cambridge)

Volumn 144, Issue 19, 2017, Pages 3625-3632

  Adam, Mike ^a   Potter, Andrew S ^a   Potter, S Steven ^a  

^a CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER   (United States)

Author keywords

Artifacts; Cell dissociation; Kidney development; RNA seq; Single cell

Indexed keywords

PROTEINASE; PEPTIDE HYDROLASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACILLUS LICHENIFORMIS; ENZYME ACTIVITY; GENE EXPRESSION; IN VIVO STUDY; INCUBATION TEMPERATURE; KIDNEY DEVELOPMENT; MOUSE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RNA SEQUENCE; SINGLE CELL ANALYSIS; ANIMAL; ARTIFACT; CYTOLOGY; EMBRYOLOGY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; KIDNEY; KIDNEY PROXIMAL TUBULE; METABOLISM; ORGANOGENESIS; PROCEDURES; SEQUENCE ANALYSIS; STROMA CELL; TEMPERATURE; TIME FACTOR;

ANIMALS; ARTIFACTS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; KIDNEY; KIDNEY TUBULES, PROXIMAL; MICE; ORGANOGENESIS; PEPTIDE HYDROLASES; SEQUENCE ANALYSIS, RNA; SINGLE-CELL ANALYSIS; STROMAL CELLS; TEMPERATURE; TIME FACTORS;

EID: 85030675342     PISSN: 09501991     EISSN: 14779129     Source Type: Journal    
DOI: 10.1242/dev.151142     Document Type: Article

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