Imaging individual mRNA molecules using multiple singly labeled probes

Nature Methods

Volumn 5, Issue 10, 2008, Pages 877-879

  Raj, Arjun ^a   van den Bogaard, Patrick ^b   Rifkin, Scott A ^a   van Oudenaarden, Alexander ^a   Tyagi, Sanjay ^b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b PUBLIC HEALTH RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; OLIGONUCLEOTIDE;

ARTICLE; DROSOPHILA; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE LOCATION; GENE NUMBER; GENE TARGETING; GENETIC ALGORITHM; GENOME ANALYSIS; HUMAN; MAMMAL CELL; MOLECULAR IMAGING; MOLECULAR PROBE; NEMATODE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA BINDING; RNA DEGRADATION; RNA TRANSLATION; SENSITIVITY ANALYSIS; SPECIES IDENTIFICATION; TANDEM REPEAT; YEAST;

ANIMALS; CAENORHABDITIS ELEGANS; DROSOPHILA MELANOGASTER; IN SITU HYBRIDIZATION, FLUORESCENCE; MAMMALS; MOLECULAR PROBES; RNA, MESSENGER;

MAMMALIA; NEMATODA;

EID: 53349161901     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth.1253     Document Type: Article

References (14)

1. Kaufmann, B.B. & van Oudenaarden, 4. Curr. Opin. Genet. Dev. 17 107-112 (2007).
2. St Johnston, D. Nat Rev. Mol. Cell Biol. 6, 363-375 (2005).
3. Gall, J.G. Proc. Natl. Acad. Sci. USA 60, 553-560 (1968).
4. Levsky, J.M. & Singer, R.H. J. Cell Sci. 116, 2833-2838 (2003).
5. Raap, A.K. et al. Hum. Mol. Genet. 4, 529-534 (1995).
6. Femino, A.M., Fay, F.S., Fogarty, K. & Singer, R.H. Science 280 585-590 (1998).
7. Maamar, H., Raj, A. & Dubnau, D. Science 327, 526-529 (2O07).
8. Femino, A.M., Fogarty, K., Lifshitz, LM., Carrington, W. & Singer, R.H. Methods Enzymol. 361, 245-304 (2003).
9. Randolph, J.B. & Waggoner, A.S. Nucleic Acids Res. 25, 2923-2929 (1997).
10. Vargas, D.Y., Raj, A., Marras, S.A., Kramer, F.R. & Tyagi, S. Proc. Natl. Acad. Sci. USA 102, 17008-17013 (2005).
11. Raj, A., Peskin, C.S., Tranchina, D., Vargas, D.Y. & Tyagi, S. PLoS Biol. 4, e309 (2006).
12. Wang, J.C. et al. Proc. Natl. Acad. Sci. USA 101, 15603-15608 (2004).
13. Fukushige, T., Hawkins, M.G. & McGhee, J.D. Dev. Biol. 198, 286-302 (1998).
14. Sanicola, M., Sekelsky, J., Elson, S. & Gelbart, W.M. Genetics 139, 745-756 (1995).