The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway

Metabolic Engineering

Volumn 43, Issue , 2017, Pages 37-45

  Zhu, Xinna ^a,b   Zhao, Dongdong ^a,b   Qiu, Huanna ^a,b,c   Fan, Feiyu ^a,b   Man, Shuli ^c   Bi, Changhao ^a,b   Zhang, Xueli ^a,b  

^a Tianjin Airport Economic Area   (China)

^b Key Laboratory Systems Biotechnology   (China)

^c TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Combinatorial metabolic engineering; CRISPR Cas9; CRISPR Cas9 facilitated multiplex pathway optimization (CFPO); Genome editing

Indexed keywords

CHROMOSOMES; DNA; DNA SEQUENCES; ESCHERICHIA COLI; GENE EXPRESSION; GENES; METABOLIC ENGINEERING; METABOLISM;

COMBINATORIAL LIBRARY; CONSTRUCTION STRATEGIES; CRISPR/CAS9; CRISPR/CAS9-FACILITATED MULTIPLEX PATHWAY OPTIMIZATION (CFPO); OPTIMAL COMBINATION; REGULATORY SEQUENCES; TRANSCRIPTIONAL UNITS; XYLOSE UTILIZATIONS;

GENE EXPRESSION REGULATION;

TRANSCRIPTOME; XYLOSE; ESCHERICHIA COLI PROTEIN;

ARTICLE; COMBINATORIAL LIBRARY; CRISPR-CAS9 SYSTEM; DNA LIBRARY; ENZYME ASSAY; ESCHERICHIA COLI; GENE EXPRESSION; GLUCOSE UTILIZATION; METABOLISM; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SPONTANEOUS MUTATION; CRISPR CAS SYSTEM; GENETICS; METABOLIC ENGINEERING;

CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; METABOLIC ENGINEERING; PLASMIDS; XYLOSE;

EID: 85029106382     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2017.08.003     Document Type: Article

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