A novel assay system for the measurement of transketolase activity using xylulokinase from Saccharomyces cerevisiae

Biotechnology Letters

Volumn 30, Issue 5, 2008, Pages 899-904

  Lee, Jin Young ^a,b   Cheong, Dae Eun ^c   Kim, Geun Joong ^c  

^a Inha University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Chonnam National University   (South Korea)

Author keywords

Coupling assay; Recombinant enzyme; Transketolase; Xylulokinase

Indexed keywords

COUPLING ASSAY; RECOMBINANT ENZYMES; TRANSKETOLASE; XYLULOKINASE;

ENZYME ACTIVITY; ESCHERICHIA COLI; PHOSPHATES; PROTEINS; REACTION KINETICS; SYNTHESIS (CHEMICAL);

BIOASSAY;

PENTOSE PHOSPHATE; PHOSPHOTRANSFERASE; RIBOSE 5 PHOSPHATE; RIBOSE PHOSPHATE; RIBOSE-5-PHOSPHATE; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSKETOLASE; UNCLASSIFIED DRUG; XYLULOKINASE; XYLULOSE; XYLULOSE 5 PHOSPHATE; XYLULOSE-5-PHOSPHATE;

ARTICLE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; PLASMID; SACCHAROMYCES CEREVISIAE;

CLONING, MOLECULAR; ESCHERICHIA COLI; PENTOSEPHOSPHATES; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; RIBOSEMONOPHOSPHATES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSKETOLASE; XYLULOSE;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 41549085722     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9616-y     Document Type: Article

References (14)

1. Ahmad S, Scopes RK (2002) Isolation and properties of a constitutive d-xylulokinase from a novel thermophilic Saccharococcus caldoxylosilyticus DSM 12041 (ATCC 700356). Enzyme Microb Technol 30:627-632
2. Boros LG, Lee WN, Go VW (2002) A metabolic hypothesis of cell growth and death in pancreatic cancer. Pancreas 24:26-33
3. French C, Ward JM (1995) Improved production and stability of E. coli recombinants expressing transketoalse for large scale biotransformation. Biotechnol Lett 17:247-252
4. Hobbs GR, Mitra RK, Chauhan RP et al (1996) Enzyme-catalyzed carbon-carbon bond formation: large scale production of Escherichia coli transketolase. J Biotechnol 45:173-179
5. Josephson BL, Fraenkel DG (1974) Sugar metabolism in transketolase mutants of Escherichia coli. J Bacteriol 118:1082-1089
6. Kamada N, Yasuhara A, Takano Y, Nakano T, Ikeda M (2001) Effect of transketolase modifications on carbon flow to the purine-nucleotide pathway in Corynebacterium ammoniagenes. Appl Microbiol Biotechnol 56:710-717
7. Lee TH, Kim MD, Park YC et al (2003) Effects of xylulokinase activity on ethanol production from d-xylulose by recombinant Saccharomyces cerevisiae. J Appl Microbiol 95:847-852
8. Richard P, Toivari MH, Penttila M (2000) The role of xylulokinase in Saccaromyces cerevisiae xylulose catabolism. FEMS Microbiol Lett 190:39-43
9. Rodriguez-Pena JM, Cid VJ, Arroyo J, Nombela C (1998) The YGR194c (XKS1) gene encodes the xylulokinase from the budding yeast Saccharomyces cerevisiae. FEMS Microbiol Let 162:155-160
10. Sevestre A, Helaine V, Guyot G, Martin C, Hecquet L (2003) A fluorogenic assay for transketolase from Saccharomyces cerevisiae. Tetrahedron Lett 44:827-830
11. Smith ME, Kaulmann U, Ward JM, Hailes HC (2006) A colorimetirc assay for screening transketolase activity. Bioorg Med Chem 14:7062-7065
12. Soh Y, Song BJ, Jeng J, Kallarakal AT (1998) Critical role of Arg433 in rat transketolase activity as probed by site-directed mutagenesis. Biochem J 333:367-372
13. Wikner C, Meshalkina L, Nilsson U et al (1994) Analysis of an invariant cofactor-protein interaction in thiamin diphosphate-dependent enzymes by site-directed mutagenesis. Glutamic acid 418 in transketolase is essential for catalysis. J Biol Chem 269:32144-32150
14. Zimmermann FT, Schneider A, Schörken U et al (1999) Efficient multi-enzymatic synthesis of d-xylulose 5-phosphate. Tetrahedron Asymmetry 10:1643-1646