Characterization of oseltamivir-resistant influenza virus populations in immunosuppressed patients using digital-droplet PCR: Comparison with qPCR and next generation sequencing analysis

Antiviral Research

Volumn 145, Issue , 2017, Pages 160-167

  Pichon, Maxime ^a,b   Gaymard, Alexandre ^a,b   Josset, Laurence ^a,b   Valette, Martine ^a   Millat, Gilles ^a   Lina, Bruno ^a,b   Escuret, Vanessa ^a,b  

^a HOSPICES CIVILS DE LYON   (France)

^b UNIVERSITÉ LYON 1   (France)

Author keywords

H275Y substitution; Influenza viruses; Next generation sequencing; Oseltamivir resistance; RT droplet digital PCR; RT qPCR

Indexed keywords

4 ACETAMIDO 5 AMINO 3 (1 ETHYLPROPOXY) 1 CYCLOHEXENE 1 CARBOXYLIC ACID; OSELTAMIVIR; SODIUM ION; VIRUS SIALIDASE; ZANAMIVIR; ANTIVIRUS AGENT; SIALIDASE; VIRAL PROTEIN;

ADULT; ANALYTICAL EQUIPMENT; ANTIVIRAL RESISTANCE; ARTICLE; B CELL LYMPHOMA; CASE REPORT; CHILD; CONTROLLED STUDY; DROPLET DIGITAL POLYMERASE CHAIN REACTION; HUMAN; IMMUNE DEFICIENCY; INFLUENZA A (H1N1); INFLUENZA A VIRUS (H1N1); INTERMETHOD COMPARISON; MALE; MIDDLE AGED; NEXT GENERATION SEQUENCING; NONHUMAN; PRESCHOOL CHILD; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEVERE COMBINED IMMUNODEFICIENCY; VIRUS LOAD; VIRUS SHEDDING; COMPARATIVE STUDY; DRUG EFFECTS; GENETICS; HIGH THROUGHPUT SEQUENCING; IMMUNOCOMPROMISED PATIENT; INFLUENZA; MISSENSE MUTATION; MOLECULAR DIAGNOSIS; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; VIROLOGY;

ANTIVIRAL AGENTS; DRUG RESISTANCE, VIRAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; IMMUNOCOMPROMISED HOST; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA, HUMAN; MOLECULAR DIAGNOSTIC TECHNIQUES; MUTATION, MISSENSE; NEURAMINIDASE; OSELTAMIVIR; REAL-TIME POLYMERASE CHAIN REACTION; VIRAL PROTEINS;

EID: 85027997401     PISSN: 01663542     EISSN: 18729096     Source Type: Journal    
DOI: 10.1016/j.antiviral.2017.07.021     Document Type: Article

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