Droplet digital PCR (ddPCR) vs quantitative real-time PCR (qPCR) approach for detection and quantification of Merkel cell polyomavirus (MCPyV) DNA in formalin fixed paraffin embedded (FFPE) cutaneous biopsies

Journal of Virological Methods

Volumn 246, Issue , 2017, Pages 15-20

  Arvia, Rosaria ^a   Sollai, Mauro ^b   Pierucci, Federica ^b   Urso, Carmelo ^c   Massi, Daniela ^b   Zakrzewska, Krystyna ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b UNIVERSITY OF FLORENCE   (Italy)

^c SANTA MARIA ANNUNZIATA HOSPITAL   (Italy)

Author keywords

ddPCR; FFPE skin samples; Merkel cell polyomavirus; Real time qPCR

Indexed keywords

VIRUS DNA; FORMALDEHYDE; PARAFFIN; PRIMER DNA;

ANALYTICAL PARAMETERS; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; COPY NUMBER VARIATION; DNA EXTRACTION; DNA PURIFICATION; DROPLET DIGITAL POLYMERASE CHAIN REACTION; ECCRINE POROCARCINOMA; GENE AMPLIFICATION; GENE DOSAGE; HOUSEKEEPING GENE; HUMAN; HUMAN TISSUE; LIMIT OF DETECTION; LINEARITY; MEASUREMENT REPEATABILITY; MERKEL CELL POLYOMAVIRUS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RISK FACTOR; SENSITIVITY ANALYSIS; SKIN BIOPSY; SQUAMOUS CELL CARCINOMA; VIRUS GENOME; VIRUS IDENTIFICATION; VIRUS LOAD; BIOPSY; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; MERKEL CELL CARCINOMA; PATHOLOGY; POLYMERASE CHAIN REACTION; POLYOMAVIRUS INFECTION; PROCEDURES; SENSITIVITY AND SPECIFICITY; SKIN; SKIN TUMOR; VIROLOGY;

BIOPSY; CARCINOMA, MERKEL CELL; DNA PRIMERS; DNA, VIRAL; FORMALDEHYDE; HUMANS; MERKEL CELL POLYOMAVIRUS; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; POLYOMAVIRUS INFECTIONS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SKIN; SKIN NEOPLASMS; VIRAL LOAD;

EID: 85018905252     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2017.04.003     Document Type: Article

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