Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR: Clinical implications for quantification of Oseltamivir-resistant subpopulations

Journal of Virological Methods

Volumn 224, Issue , 2015, Pages 58-66

  Taylor, Sean C ^a   Carbonneau, Julie ^b   Shelton, Dawne N ^c   Boivin, Guy ^b  

^a Bio Rad Laboratories Canada Inc   (Canada)

^b LAVAL UNIVERSITY   (Canada)

^c BIO RAD LABORATORIES   (United States)

Author keywords

DdPCR; Droplet Digital PCR; Experimental optimization; Influenza virus; RT qPCR

Indexed keywords

DNA; NUCLEIC ACID; OSELTAMIVIR; RNA; SIALIDASE; ANTIVIRUS AGENT; NA PROTEIN, INFLUENZA A VIRUS; VIRUS PROTEIN;

2009 H1N1 INFLUENZA; ARTICLE; CHILD; CONTROLLED STUDY; DNA EXTRACTION; DROPLET DIGITAL POLYMERASE CHAIN REACTION; HUMAN; LIMIT OF QUANTITATION; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY ANALYSIS; VIRUS MUTATION; VIRUS RESISTANCE; WILD TYPE; ANTIVIRAL RESISTANCE; CLASSIFICATION; COMPARATIVE STUDY; DRUG EFFECTS; EVALUATION STUDY; GENETICS; INFLUENZA; INFLUENZA A VIRUS (H1N1); ISOLATION AND PURIFICATION; MALE; PRESCHOOL CHILD; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VIROLOGY; VIRUS LOAD;

ANTIVIRAL AGENTS; CHILD, PRESCHOOL; DRUG RESISTANCE, VIRAL; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA, HUMAN; MALE; NEURAMINIDASE; OSELTAMIVIR; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; VIRAL LOAD; VIRAL PROTEINS;

EID: 84940650290     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2015.08.014     Document Type: Article

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