Obtaining genomes from uncultivated environmental microorganisms using FACS-based single-cell genomics

Nature Protocols

Volumn 9, Issue 5, 2014, Pages 1038-1048

  Rinke, Christian ^a   Lee, Janey ^a   Nath, Nandita ^a   Goudeau, Danielle ^a   Thompson, Brian ^b   Poulton, Nicole ^b   Dmitrieff, Elizabeth ^b   Malmstrom, Rex ^a   Stepanauskas, Ramunas ^b   Woyke, Tanja ^a  

^a DOE JOINT GENOME INSTITUTE   (United States)

^b BIGELOW LABORATORY FOR OCEAN SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; RNA 16S;

ARCHAEON; ARTICLE; BACTERIAL CELL; CELL ISOLATION; CELL SEPARATION; CONTROLLED STUDY; CYTOLYSIS; DNA DEGRADATION; DNA MICROARRAY; FLUORESCENCE ACTIVATED CELL SORTING; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC VARIABILITY; GENOME ANALYSIS; GENOMICS; HIGH THROUGHPUT SEQUENCING; MICROBIAL GENETICS; MICROBIAL GENOME; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA ANALYSIS; SEQUENCE ANALYSIS; SINGLE CELL ANALYSIS; FLOW CYTOMETRY; GENETICS; MICROFLORA; NUCLEIC ACID AMPLIFICATION; PROCEDURES;

CELL SEPARATION; FLOW CYTOMETRY; GENOMICS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MICROBIOTA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; RNA, RIBOSOMAL, 16S; SINGLE-CELL ANALYSIS;

EID: 84899507119     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.067     Document Type: Article

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