Increasing Malonyl-CoA Derived Product through Controlling the Transcription Regulators of Phospholipid Synthesis in Saccharomyces cerevisiae

ACS Synthetic Biology

Volumn 6, Issue 5, 2017, Pages 905-912

  Chen, Xiaoxu ^a   Yang, Xiaoyu ^a   Shen, Yu ^a   Hou, Jin ^a   Bao, Xiaoming ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

3 hydroxypropionic acid; INO2; INO4; malonyl CoA; Saccharomyces cerevisiae

Indexed keywords

CHOLINE; HYDRACRYLIC ACID; INOSITOL; MALONYL COENZYME A; PROTEIN INO2P; PROTEIN INO4P; PROTEIN OPI1P; PROTEIN VARIANT; REGULATOR PROTEIN; REPRESSOR PROTEIN; UNCLASSIFIED DRUG; LACTIC ACID; PHOSPHOLIPID; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CELL GROWTH; CONTROLLED STUDY; DNA BINDING MOTIF; FUNGAL CELL; FUNGAL STRAIN; GENE DELETION; GENE MUTATION; INO2 GENE; INO4 GENE; NONHUMAN; PHOSPHOLIPID SYNTHESIS; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; SACCHAROMYCES CEREVISIAE; ANALOGS AND DERIVATIVES; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHYSIOLOGY;

GENE EXPRESSION REGULATION, FUNGAL; LACTIC ACID; MALONYL COENZYME A; PHOSPHOLIPIDS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 85019544318     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00346     Document Type: Article

References (30)

1. Dixon, R. A. and Steele, C. L. (1999) Flavonoids and isoflavonoids-a gold mine for metabolic engineering Trends Plant Sci. 4, 394-400 10.1016/S1360-1385(99)01471-5
2. Griffin, M. O., Fricovsky, E., Ceballos, G., and Villarreal, F. (2010) Tetracyclines: a pleitropic family of compounds with promising therapeutic properties. Review of the literature American Journal of Physiology-Cell Physiology 299, C539-C548 10.1152/ajpcell.00047.2010
3. Han, A. R., Park, J. W., Lee, M. K., Ban, Y. H., Yoo, Y. J., Kim, E. J., Kim, E., Kim, B. G., Sohng, J. K., and Yoon, Y. J. (2011) Development of a Streptomyces venezuelae-based combinatorial biosynthetic system for the production of glycosylated derivatives of doxorubicin and its biosynthetic intermediates Appl. Environ. Microbiol. 77, 4912-4923 10.1128/AEM.02527-10
4. Leonard, E., Yan, Y., Fowler, Z. L., Li, Z., Lim, C. G., Lim, K. H., and Koffas, M. A. (2008) Strain improvement of recombinant Escherichia coli for efficient production of plant flavonoids Mol. Pharmaceutics 5, 257-265 10.1021/mp7001472
5. Edwards, M. L., Stemerick, D. M., and Sunkara, P. S. (1990) Chalcones: a new class of antimitotic agents J. Med. Chem. 33, 1948-1954 10.1021/jm00169a021
6. Zha, W., Rubin-Pitel, S. B., Shao, Z., and Zhao, H. (2009) Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering Metab. Eng. 11, 192-198 10.1016/j.ymben.2009.01.005
7. Kalscheuer, R., Stölting, T., and Steinbüchel, A. (2006) Microdiesel: Escherichia coli engineered for fuel production Microbiology 152, 2529-2536 10.1099/mic.0.29028-0
8. Shi, S., Chen, Y., Siewers, V., and Nielsen, J. (2014) Improving production of malonyl coenzyme A-derived metabolites by abolishing Snf1-dependent regulation of Acc1 mBio 5, e01130-01114 10.1128/mBio.01130-14
9. Li, X., Guo, D., Cheng, Y., Zhu, F., Deng, Z., and Liu, T. (2014) Overproduction of fatty acids in engineered Saccharomyces cerevisiae Biotechnol. Bioeng. 111, 1841-1852 10.1002/bit.25239
10. Wattanachaisaereekul, S., Lantz, A. E., Nielsen, M. L., and Nielsen, J. (2008) Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA supply Metab. Eng. 10, 246-254 10.1016/j.ymben.2008.04.005
11. Jin, W. C. and Silva, N. A. D. (2014) Improving polyketide and fatty acid synthesis by engineering of the yeast acetyl-CoA carboxylase J. Biotechnol. 187, 56-59 10.1016/j.jbiotec.2014.07.430
12. Wang, Y., Chen, H., and Yu, O. (2014) A plant malonyl-CoA synthetase enhances lipid content and polyketide yield in yeast cells Appl. Microbiol. Biotechnol. 98, 5435-5447 10.1007/s00253-014-5612-z
13. Cardenas, J. and Silva, N. A. D. (2014) Metabolic engineering of Saccharomyces cerevisiae for the production of triacetic acid lactone Metab. Eng. 25, 194-203 10.1016/j.ymben.2014.07.008
14. Li, S., Si, T., Wang, M., and Zhao, H. (2015) Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening ACS Synth. Biol. 4, 1308-1315 10.1021/acssynbio.5b00069
15. Schüller, H. J., Hahn, A., Tröster, F., Schütz, A., and Schweizer, E. (1992) Coordinate genetic control of yeast fatty acid synthase genes FAS1 and FAS2 by an upstream activation site common to genes involved in membrane lipid biosynthesis EMBO J. 11, 107-114
16. Schwank, S., Ebbert, R., Rautenstrauss, K., Schweizer, E., and Schüller, H. J. (1995) Yeast transcriptional activator INO2 interacts as an Ino2p/Ino4p basic helix-loop-helix heteromeric complex with the inositol/choline Nucleic Acids Res. 23, 230-237 10.1093/nar/23.2.230
17. Wagner, C., Dietz, M., Wittmann, J., Albrecht, A., and Schüller, H. J. (2001) The negative regulator Opi1 of phospholipid biosynthesis in yeast contacts the pleiotropic repressor Sin3 and the transcriptional activator Ino2 Mol. Microbiol. 41, 155-166 10.1046/j.1365-2958.2001.02495.x
18. Loewen, C. J. R., Roy, A., and Levine, T. P. (2003) A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP EMBO J. 22, 2025-2035 10.1093/emboj/cdg201
19. Loewen, C. J. R., Gaspar, M. L., Jesch, S. A., Delon, C., Ktistakis, N. T., Henry, S. A., and Levine, T. P. (2004) Phospholipid Metabolism Regulated by a Transcription Factor Sensing Phosphatidic Acid Science 304, 1644-1647 10.1126/science.1096083
20. Ambroziak, J. and Henry, S. A. (1994) INO2 and INO4 gene products, positive regulators of phospholipid biosynthesis in Saccharomyces cerevisiae, form a complex that binds to the INO1 promoter J. Biol. Chem. 269, 15344-15349
21. Henry, S. A., Kohlwein, S. D., and Carman, G. M. (2012) Metabolism and Regulation of Glycerolipids in the Yeast Saccharomyces cerevisiae Genetics 190, 317-349 10.1534/genetics.111.130286
22. Hirsch, J. P. and Henry, S. A. (1986) Expression of the Saccharomyces cerevisiae inositol-1-phosphate synthase (INO1) gene is regulated by factors that affect phospholipid synthesis Mol. Cell. Biol. 6, 3320-3328 10.1128/MCB.6.10.3320
23. Chen, M., Hancock, L. C., and Lopes, J. M. (2007) Transcriptional regulation of yeast phospholipid biosynthetic genes Biochim. Biophys. Acta, Mol. Cell Biol. Lipids 1771, 310-321 10.1016/j.bbalip.2006.05.017
24. Koipally, J., Ashburner, B. P., Bachhawat, N., Gill, T., Hung, G., Henry, S. A., and Lopes, J. M. (1996) Functional characterization of the repeated UASINO element in the promoters of the INO1 and CHO2 genes of yeast Yeast 12, 653-665 10.1002/(SICI)1097-0061(19960615)12:7<653::AID-YEA953>3.0.CO;2-T
25. David, F., Nielsen, J., and Siewers, V. (2016) Flux control at the malonyl-CoA node through hierarchical dynamic pathway regulation in Saccharomyces cerevisiae ACS Synth. Biol. 9, 785-796 10.1021/acssynbio.5b00161
26. Dietz, M., Heyken, W. T., Hoppen, J., Geburtig, S., and Schüller, H. J. (2003) TFIIB and subunits of the SAGA complex are involved in transcriptional activation of phospholipid biosynthetic genes by the regulatory protein Ino2 in the yeast Saccharomyces cerevisiae Mol. Microbiol. 48, 1119-1130 10.1046/j.1365-2958.2003.03501.x
27. Nikoloff, D. M. and Henry, S. A. (1994) Functional characterization of the INO2 gene of Saccharomyces cerevisiae. A positive regulator of phospholipid biosynthesis J. Biol. Chem. 269, 7402-7411
28. Kildegaard, K. R., Jensen, N. B., Schneider, K., Czarnotta, E., Özdemir, E., Klein, T., Maury, J., Ebert, B. E., Christensen, H. B., and Yun, C. (2016) Engineering and systems-level analysis of Saccharomyces cerevisiae for production of 3-hydroxypropionic acid via malonyl-CoA reductase-dependent pathway Microb. Cell Fact. 15, 1-13 10.1186/s12934-016-0451-5
29. Hou, J., Fan, S., Wang, C., Li, X., Yu, S., and Bao, X. (2014) Fine-tuning of NADH oxidase decreases byproduct accumulation in respiration deficient xylose metabolic Saccharomyces cerevisiae BMC Biotechnol. 14, 1-10 10.1186/1472-6750-14-13
30. Shi, S., Valle-Rodríguez, J. O., Khoomrung, S., Siewers, V., and Nielsen, J. (2012) Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production Biotechnol. Biofuels 5, 7 10.1186/PREACCEPT-1932279820621895