Improving polyketide and fatty acid synthesis by engineering of the yeast acetyl-CoA carboxylase

Journal of Biotechnology

Volumn 187, Issue , 2014, Pages 56-59

  Choi, Jin Wook ^a   Da Silva, Nancy A ^a  

^a University of California at Irvine   (United States)

Author keywords

6 MSA; Acc1; Fatty acids; Phosphorylation; Polyketides; Saccharomyces cerevisiae

Indexed keywords

AMINO ACIDS; ENZYMES; FATTY ACIDS; GLUCOSE; KETONES; PHOSPHORYLATION; YEAST;

6-MSA; ACC1; ACETYL-COA CARBOXYLASE; CRITICAL AMINO ACIDS; FATTY ACID SYNTHESIS; GLUCOSE DEPLETION; POLYKETIDES; SPECIFIC ACTIVITY;

YEAST; FATTY ACIDS;

6 METHYLSALISYLIC ACID; ACETYL COENZYME A CARBOXYLASE; ALANINE; ALCOHOL; FATTY ACID; GLUCOSE; MUTANT PROTEIN; POLYKETIDE; UNCLASSIFIED DRUG; SACCHAROMYCES CEREVISIAE PROTEIN; SALICYLIC ACID DERIVATIVE; SALICYLIC ACID METHYL ESTER; AMINO ACID; MALONYL COENZYME A; PROTEIN SERINE THREONINE KINASE;

ARTICLE; BIOSYNTHESIS; CELL LEVEL; CONTROLLED STUDY; CYTOSOL; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INACTIVATION; ENZYME PHOSPHORYLATION; FATTY ACID SYNTHESIS; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; YEAST; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; ENZYMOLOGY; METABOLISM; MOLECULAR GENETICS; PROTEIN ENGINEERING; RAT; SEQUENCE ALIGNMENT; ENZYME ENGINEERING; MUTATION; RATTUS NORVEGICUS; SYNTHESIS;

ACETYL-COA CARBOXYLASE; AMINO ACID SEQUENCE; ANIMALS; FATTY ACIDS; MOLECULAR SEQUENCE DATA; POLYKETIDES; PROTEIN ENGINEERING; RATS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SALICYLATES; SEQUENCE ALIGNMENT;

EID: 84905818450     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.07.430     Document Type: Article

References (28)

1. Chen Y., Bao J., Kim I.K., Siewers V., Nielsen J. Coupled incremental precursor and co-factor supply improves 3-hydroxypropionic acid production in Saccharomyces cerevisiae. Metab. Eng. 2014, 22:104-109.
2. Chia M., Schwartz T.J., Shanks B.H., Dumesic J.A. Triacetic acid lactone as a potential biorenewable platform chemical. Green Chem. 2012, 14:1850-1853.
3. Chooi Y.H., Tang Y. Navigating the fungal polyketide chemical space: from genes to molecules. J. Org. Chem. 2012, 77:9933-9953.
4. Crawford J.M., Townsend C.A. New insights into the formation of fungal aromatic polyketides. Nat. Rev. Microbiol. 2010, 8:879-889.
5. Dale S., Wilson W.A., Edelman A.M., Hardie D.G. Similar substrate recognition motifs for mammalian AMP-activated protein kinase, higher plant HMG-CoA reductase kinase-A, yeast SNF1, and mammalian calmodulin- dependent protein kinase I. Febs Lett. 1995, 361:191-195.
6. Davies S.P., Sim A.T.R., Hardie D.G. Location and function of three sites phosphorylated on rat acetyl-CoA carboxylase by the AMP-activated protein kinase. Eur. J. Biochem. 1990, 187:183-190.
7. Dimroth P., Walter H., Lynen F. Biosynthesis of 6-methylsalicylic acid. Eur. J. Biochem. 1970, 13:98-110.
8. Eckermann S., Schroder G., Schmidt J., Strack D., Edrada R.A., Helariutta Y., Elomaa P., Kotilainen M., Kilpelainen I., Proksch P., Teeri T.H., Schroder J. New pathway to polyketides in plants. Nature 1998, 396:387-390.
9. Fortman J.L., Chhabra S., Mukhopadhyay A., Chou H., Lee T.S., Steen E., Keasling J.D. Biofuel alternatives to ethanol: pumping the microbial well. Trends Biotechnol. 2008, 26:375-381.
10. Ha J., Daniel S., Broyles S.S., Kim K.H. Critical phosphorylation sites for acetyl-CoA carboxylase activity. J. Biol. Chem. 1994, 269:22162-22168.
11. Jones E.W. Tackling the protease problem in Saccharomyces cerevisiae. Method. Enzymol. 1991, 194:428-453.
12. Kealey J.T., Liu L., Santi D.V., Betlach M.C., Barr P.J. Production of a polyketide natural product in nonpolyketide-producing prokaryotic and eukaryotic hosts. Proc. Natl. Acad. Sci. USA 1998, 95:505-509.
13. Kennedy J., Auclair K., Kendrew S.G., Park C., Vederas J.C., Hutchinson C.R. Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis. Science 1999, 284:1368-1372.
14. Kozak B.U., van Rossum H.M., Benjamin K.R., Wu L., Daran J.M.G., Pronk J.T., van Maris A.I.J.A. Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases by alternative pathways for cytosolic acetyl-CoA synthesis. Metab. Eng. 2014, 21:46-59.
15. Leber C., Da Silva N.A. Engineering of Saccharomyces cerevisiae for the synthesis of short chain fatty acids. Biotechnol. Bioeng. 2014, 111:347-358.
16. Ma S.M., Li J.W.H., Choi J.W., Zhou H., Lee K.K.M., Moorthie V.A., Xie X.K., Kealey J.T., Da Silva N.A., Vederas J.C., Tang Y. Complete reconstitution of a highly reducing iterative polyketide synthase. Science 2009, 326:589-592.
17. Munday M.R., Campbell D.G., Carling D., Hardie D.G. Identification by amino acid sequencing of three major regulatory phosphorylation sites on rat acetyl-CoA carboxylase. Eur. J. Biochem. 1988, 175:331-338.
18. Nikolau B.J. An integrated strategy for generating lipid-based biorenewable chemicals: diversifying fatty acid synthesis with polyketide synthesis biocatalysts. Chem. Phys. Lipids 2010, 163:S16-S17.
19. Scott J.W., Norman D.G., Hawley S.A., Kontogiannis L., Hardie D.G. Protein kinase substrate recognition studied using the recombinant catalytic domain of AMP-activated protein kinase and a model substrate. J. Mol. Biol. 2002, 317:309-323.
20. Shi S., Chen Y., Siewers V., Nielsen J. Improving production of malonyl coenzyme A-derived metabolites by abolishing Snf1-dependent regulation of Acc1. mBio. 2014, 5:e01130-e1214.
21. Shiba Y., Paradise E.M., Kirby J., Ro D.K., Keasing J.D. Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids. Metab. Eng. 2007, 9:160-168.
22. Shirra M.K., Patton-Vogt J., Ulrich A., Liuta-Tehlivets O., Kohlwein S.D., Henry S.A., Arndt K.M. Inhibition of acetyl coenzyme A carboxylase activity restores expression of the INO1 gene in a snf1 mutant strain of Saccharomyces cerevisiae. Mol. Cell. Biol. 2001, 21:5710-5722.
23. Spencer J.B., Jordan P.M. Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum. Biochem. J. 1992, 288:839-846.
24. Vogel G., Lynen F. 6-Methylsalicylic acid synthetase. Method. Enzymol. United States 1975, vol. 43:520-530.
25. Wattanachaisaereekul S., Lantz A.E., Nielsen M.L., Nielsen J. Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA supply. Metab. Eng. 2008, 10:246-254.
26. Weekes J., Ball K.L., Caudwell F.B., Hardie D.G. Specificity determinants for the AMP-activated protein kinase and its plant homolog analyzed using synthetic peptides. Febs Lett. 1993, 334:335-339.
27. Woods A., Munday M.R., Scott J., Yang X.L., Carlson M., Carling D. Yeast SNF1 is functionally related to mammalian AMP-activated protein kinase and regulates acetyl-CoA carboxylase in vivo. J. Biol. Chem. 1994, 269:19509-19515.
28. Xu W., Chooi Y.H., Choi J.W., Li S., Vederas J.C., Da Silva N.A., Tang Y. LovG: The thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis. Angew. Chem. Int. Ed. Engl. 2013, 52:6472-6475.