MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes

Methods in Enzymology

Volumn 592, Issue , 2017, Pages 1-26

  Gradia, Scott D ^a,e   Ishida, Justin P ^b,f   Tsai, Miaw Sheue ^b   Jeans, Chris ^a   Tainer, John A ^b,c   Fuss, Jill O ^b,d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^d Cinder Biological Inc   (United States)

^e Caribou Biosciences Inc   (United States)

^f BIOMARIN PHARMACEUTICAL INC   (United States)

Author keywords

Baculovirus; Biobricks; Insect cells; LIC; MacroBac; Multigene; Protein complex; Recombinant protein expression

Indexed keywords

BACMID; CIRCULAR DNA; MULTIPROTEIN COMPLEX; RESTRICTION ENDONUCLEASE; RECOMBINANT PROTEIN;

CLONING VECTOR; DNA REPAIR; ENZYME METABOLISM; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENE INSERTION; GENE ORDER; MACROBAC; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; ANIMAL; BACULOVIRIDAE; CYTOLOGY; GENE VECTOR; GENETICS; HUMAN; INSECT; MOLECULAR CLONING; MULTIGENE FAMILY; NUCLEOTIDE SEQUENCE; PROCEDURES; PROMOTER REGION;

ANIMALS; BACULOVIRIDAE; BASE SEQUENCE; CLONING, MOLECULAR; GENE EXPRESSION; GENETIC VECTORS; HUMANS; INSECTA; MULTIGENE FAMILY; MULTIPROTEIN COMPLEXES; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

EID: 85019473116     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2017.03.008     Document Type: Chapter

References (23)

1. Aslanidis, C., de Jong, P.J., Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Research 18 (1990), 6069–6074.
2. Classen, S., Hura, G.L., Holton, J.M., Rambo, R.P., Rodic, I., McGuire, P.J., et al. Implementation and performance of SIBYLS: A dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the advanced light source. Journal of Applied Crystallography 46 (2013), 1–13, 10.1107/S0021889812048698.
3. Fuss, J.O., Tainer, J.A., XPB and XPD helicases in TFIIH orchestrate DNA duplex opening and damage verification to coordinate repair with transcription and cell cycle via CAK kinase. DNA Repair 10 (2011), 697–713.
4. Gibson, D.G., Young, L., Chuang, R.Y., Venter, J.C., Hutchison, C.A. 3rd, Smith, H.O., Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature Methods 6 (2009), 343–345.
5. Greimann, J.C., Lima, C.D., Reconstitution of RNA exosomes from human and Saccharomyces cerevisiae cloning, expression, purification, and activity assays. Methods in Enzymology 448 (2008), 185–210.
6. Hammel, M., Yu, Y., Radhakrishnan, S.K., Chokshi, C., Tsai, M.S., Matsumoto, Y., et al. An intrinsically disordered APLF links Ku, DNA-PKcs and XRCC4-DNA ligase IV in an extended flexible non-homologous end joining complex. The Journal of Biological Chemistry 291 (2016), 26987–27006.
7. He, Y., Yan, C., Fang, J., Inouye, C., Tjian, R., Ivanov, I., et al. Near-atomic resolution visualization of human transcription promoter opening. Nature 533 (2016), 359–365.
8. Hura, G.L., Budworth, H., Dyer, K.N., Rambo, R.P., Hammel, M., McMurray, C.T., et al. Comprehensive macromolecular conformations mapped by quantitative SAXS analyses. Nature Methods 10 (2013), 453–454.
9. Knight, T., Idempotent vector design for standard assembly of biobricks. 2003, MIT Artificial Intelligence Laboratory, MIT Synthetic Biology Working Group, Cambridge, MA.
10. Lafrance-Vanasse, J., Williams, G.J., Tainer, J.A., Envisioning the dynamics and flexibility of Mre11-Rad50-Nbs1 complex to decipher its roles in DNA replication and repair. Progress in Biophysics and Molecular Biology 117 (2015), 182–193.
11. Life_Technologies, Bac-to-Bac® baculovirus expression system. 2015, Thermo Fisher Scientific, Waltham, MA.
12. Mena, J.A., Ramírez, O.T., Palomares, L.A., Population kinetics during simultaneous infection of insect cells with two different recombinant baculoviruses for the production of rotavirus-like particles. BMC Biotechnology, 7, 2007, 39.
13. Nie, Y., Bellon-Echeverria, I., Trowitzsch, S., Bieniossek, C., Berger, I., Multiprotein complex production in insect cells by using polyproteins. Methods in Molecular Biology 1091 (2014), 131–141.
14. Rambo, R.P., Tainer, J.A., Accurate assessment of mass, models and resolution by small-angle scattering. Nature 496 (2013), 477–481.
15. Sari, D., Gupta, K., Thimiri Govinda Raj, D.B., Aubert, A., Drncova, P., Garzoni, F., et al. The MultiBac baculovirus/insect cell expression vector system for producing complex protein biologics. Advances in Experimental Medicine and Biology 896 (2016), 199–215.
16. Schultz, P., Fribourg, S., Poterszman, A., Mallouh, V., Moras, D., Egly, J.M., Molecular structure of human TFIIH. Cell 102 (2000), 599–607.
17. Smith, G.E., Summers, M.D., Fraser, M.J., Production of human beta interferon in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 3 (1983), 2156–2165.
18. Thimiri Govinda Raj, D.B., Vijayachandran, L.S., Berger, I., OmniBac: Universal multigene transfer plasmids for baculovirus expression vector systems. Methods in Molecular Biology 1091 (2014), 123–130.
19. Trowitzsch, S., Bieniossek, C., Nie, Y., Garzoni, F., Berger, I., New baculovirus expression tools for recombinant protein complex production. Journal of Structural Biology 172 (2010), 45–54.
20. Vijayachandran, L.S., Viola, C., Garzoni, F., Trowitzsch, S., Bieniossek, C., Chaillet, M., et al. Robots, pipelines, polyproteins: Enabling multiprotein expression in prokaryotic and eukaryotic cells. Journal of Structural Biology 175 (2011), 198–208.
21. Wasilko, D.J., Lee, S.E., Stutzman-Engwall, K.J., Reitz, B.A., Emmons, T.L., Mathis, K.J., et al. The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus. Protein Expression and Purification 65 (2009), 122–132.
22. Weissmann, F., Petzold, G., VanderLinden, R., Huis Veld, P.J., Brown, N.G., Lampert, F., et al. biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes. Proceedings of the National Academy of Sciences of the United States of America 113 (2016), E2564–E2569.
23. Williams, G.J., Hammel, M., Radhakrishnan, S.K., Ramsden, D., Lees-Miller, S.P., Tainer, J.A., Structural insights into NHEJ: Building up an integrated picture of the dynamic DSB repair super complex, one component and interaction at a time. DNA Repair 17 (2014), 110–120.