OmniBac: Universal multigene transfer plasmids for baculovirus expression vector systems

Methods in Molecular Biology

Volumn 1091, Issue , 2014, Pages 123-130

  Raj, Deepak B Thimiri Govinda ^a   Vijayachandran, Lakshmi S ^a,b   Berger, Imre ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (France)

^b AMRITA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Bac to Bac; BacVector series; BEVS; Co expression; Cre LoxP fusion; FlashBac; Homologous recombination; MultiBac; Multiprotein complexes; OmniBac; Tn7 transposition

Indexed keywords

PROTEIN; OMNIBAC; PLASMID VECTOR; REAGENT; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; GENE TRANSFER; GENETIC TRANSFECTION; HETEROLOGOUS EXPRESSION; HOMOLOGOUS RECOMBINATION; INSECT CELL; NONHUMAN; OMNIBAC SYSTEM; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; VIRUS GENOME; VIRUS RECOMBINANT; CONTROLLED STUDY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SYNTHESIS; WESTERN BLOTTING;

ANIMALS; BACULOVIRIDAE; GENE EXPRESSION; GENETIC VECTORS; GENOME, VIRAL; HOMOLOGOUS RECOMBINATION; PLASMIDS; VIRUS REPLICATION;

EID: 84934440437     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-691-7_7     Document Type: Article

References (15)

1. Luckow VA, Lee SC, Barry GF et al (1993) Effi cient generation of infectious recombinant baculoviruses by site-specifi c transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J Virol 67:4566-4579
2. Hitchman RB, Possee RD, Crombie AT et al (2010) Genetic modifi cation of a baculovirus vector for increased expression in insect cells. Cell Biol Toxicol 26:57-68. doi: 10.1007/s10565-009-9133-y
3. Hitchman RB, Possee RD, King LA (2009) Baculovirus expression systems for recombinant protein production in insect cells. Recent Pat Biotechnol 3:46-54
4. Possee RD, Hitchman RB, Richards KS et al (2008) Generation of baculovirus vectors for the high-throughput production of proteins in insect cells. Biotechnol Bioeng 101:1115-1122. doi: 10.1002/bit.22002
5. Kitts PA, Possee RD (1993) A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques 14:810-817
6. Berger I, Fitzgerald DJ, Richmond TJ (2004) Baculovirus expression system for heterologous multiprotein complexes. Nat Biotechnol 22:1583-1587. doi: 10.1038/nbt1036
7. Palmberger D, Wilson IB, Berger I et al (2012) SweetBac: a new approach for the production of mammalianised glycoproteins in insect cells. PLoS One 7:e34226. doi: 10.1371/journal. pone.0034226
8. Vijayachandran LS, Thimiri Govinda Raj DB, Edelweiss E et al (2013) Gene gymnastics: synthetic biology for baculovirus expression vector system engineering. Bioengineered 4:279-287
9. Fitzgerald DJ, Berger P, Schaffitzel C et al (2006) Protein complex expression by using multigene baculoviral vectors. Nat Methods 3:1021-1032. doi: 10.1038/nmeth983
10. Bieniossek C, Nie Y, Frey D et al (2009) Automated unrestricted multigene recombineering for multiprotein complex production. Nat Methods 6:447-450. doi: 10.1038/nmeth.1326
11. Bieniossek C, Imasaki T, Takagi Y et al (2012) MultiBac: expanding the research toolbox for multiprotein complexes. Trends Biochem Sci 37:49-57. doi: 10.1016/j.tibs.2011.10.005
12. Haffke M, Viola C, Nie Y et al (2013) Tandem recombineering by SLIC cloning and Cre-LoxP fusion to generate multigene expression constructs for protein complex Research. Methods Mol Biol 1073:131-140
13. Li MZ, Elledge SJ (2007) Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat Methods 4:251-256. doi: 10.1038/nmeth1010
14. Trowitzsch S, Bieniossek C, Nie Y et al (2010) New baculovirus expression tools for recombinant protein complex production. J Struct Biol 172:45-54. doi: 10.1016/j. jsb.2010.02.010
15. Vijayachandran LS, Viola C, Garzoni F et al (2011) Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells. J Struct Biol 175:198-208. doi: 10.1016/j.jsb.2011.03.007