Intake of saffron reduces γ-radiation-induced genotoxicity and oxidative stress in mice

Toxicology Mechanisms and Methods

Volumn 27, Issue 6, 2017, Pages 428-434

  Koul, Apurva ^a   Abraham, Suresh K ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

anti oxidant activity; micronuclei; Saffron; radiation

Indexed keywords

ANTIOXIDANT; CATALASE; DISTILLED WATER; GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE TRANSFERASE; HYDROXYL RADICAL; MALONALDEHYDE; PLANT EXTRACT; SAFFRON EXTRACT; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; ASSAY; BONE MARROW CELL; BRAIN; CELL FREE DNA NICKING ASSAY; CELL PROLIFERATION; CHROMOSOME DAMAGE; CONTROLLED STUDY; CROCUS SATIVUS; DNA DAMAGE; ERYTHROCYTE; FREEZE DRYING; GAMMA RADIATION; GENOTOXICITY; GERM CELL; HISTOPATHOLOGY; IN VIVO STUDY; INTESTINE CELL; INTESTINE CRYPT; INTESTINE TISSUE; LEYDIG CELL; LIPID PEROXIDATION; LIVER; MALE; MICRONUCLEATED POLYCHROMATIC ERYTHROCYTE; MICRONUCLEUS; MOUSE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; SEMINIFEROUS TUBULE; SEMINIFEROUS TUBULE EPITHELIUM; SERTOLI CELL; TESTIS; ANIMAL; CHEMICALLY INDUCED; CHEMISTRY; CROCUS; DRUG EFFECTS; ISOLATION AND PURIFICATION; RADIATION RESPONSE; SINGLE STRANDED DNA BREAK;

ANIMALS; ANTIOXIDANTS; BONE MARROW CELLS; BRAIN; CROCUS; DNA BREAKS, SINGLE-STRANDED; DNA DAMAGE; GAMMA RAYS; LIPID PEROXIDATION; LIVER; MALE; MICE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; OXIDATIVE STRESS; PLANT EXTRACTS;

EID: 85017472747     PISSN: 15376516     EISSN: 15376524     Source Type: Journal    
DOI: 10.1080/15376516.2017.1307476     Document Type: Article

References (43)

1. Abdullaev FI, Espinosa-Aguirre JJ., (2004). Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev 28:426–32.
2. Abdullaev FI, Riverón-Negrete L, Caballero-Ortegaa H, et al. (2003). Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.). Toxicol In Vitro 17:731–6.
3. Abraham SK., (1989). Inhibition of in vivo genotoxicity by coffee. Food Chem Toxicol 27:787–92.
4. Aebi H., (1984). Catalase in vitro. Meth Enzymol 105:121–6.
5. Afshari JT, Brook A, Mousavi SH., (2008). Study of cytotoxic and apoptogenic properties of saffron extract in human cancer cell lines. Food Chem Toxicol 46:3443–7.
6. Altinoz E, Oner Z, Elbe H, et al. (2015). Protective effects of saffron (its active constituent, crocin) on nephropathy in streptozotocin-induced diabetic rats. Hum Exp Toxicol 34:127–34.
7. Aprotosoaie AC, Trifan A, Gille E, et al. (2015). Can phytochemicals be a bridge to develop new radioprotective agents? Phytochem Rev 14:555–66.
8. Asai A, Nakano T, Takahashi M, et al. (2005). Orally administered crocetin and crocins are absorbed into blood plasma as crocetin and its glucuronide conjugates in mice. J Agric Food Chem 53:7302–6.
9. Bancroft D, Stevens A, Turner R., (1996). Theory and practice of histological techniques. 4th ed. Edinburgh, London, Melbourne:Churchill Livingstone.
10. Bandegi AR, Pour AR, Vafaei AA, et al. (2014). Protective effects of Crocus Sativus L. extract and crocin against chronic-stress induced oxidative damage of brain, liver and kidneys in rats. Adv Pharm Bull 4:493–9.
11. Bhandari PR., (2013). A review of radioprotective plants. Int J Green Pharm 90–101.
12. Caballero-Ortega H, Pereda-Miranda R, Abdullaev FI., (2007). HPLC quantification of major active components from 11 different saffron (Crocus sativus L.) sources. Food Chem 100:1126–31.
13. Carlberg I, Mannervik B., (1985). Glutathione reductase assay. Meth Enzymol 113:484–95.
14. Datta K, Suman S, Kallakury BVS, et al. (2012). Exposure to heavy ion radiation induces persistent oxidative stress in mouse intestine. PLoS One 7:e42224
15. Dinkova-Kostova AT., (2008). Phytochemicals as protectors against ultraviolet radiation:versatility of effects and mechanisms. Planta Med 74:1548–59.
16. Ellman GL, Courtney KD, Andres VJ, et al. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95.
17. Escribanoa J, Alonsob GL, Coca-Pradosc M, et al. (1996). Crocin, safranal and picrocrocin from saffron (Crocus sativus L.) inhibit the growth of human cancer cells in vitro. Cancer Lett 100:23–30.
18. Flohe L, Gunzler WA., (1984). Assays of glutathione peroxidase. Methods Enzymol 105:114–21.
19. Habig WH, Pabst MJ, Jakoby WB., (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–9.
20. Holme JA, Bjørge C, Trbojevic M, et al. (1998). Effects of chemical-induced DNA damage on male germ cells. Arch Toxicol Suppl 20:151–60.
21. Hosseinzadeh H, Abootorabi A, Sadeghnia HR., (2008). Protective effect of Crocus sativus stigma extract and crocin (trans-crocin 4) on methyl methanesulfonate-induced DNA damage in mice organs. DNA Cell Biol 27:657–64.
22. Hosseinzadeh H, Sadeghnia HR., (2007). Effect of safranal, a constituent of Crocus sativus (saffron), on methyl methanesulfonate (MMS)-induced DNA damage in mouse organs:an alkaline single-cell gel electrophoresis (comet) assay. DNA Cell Biol 26:841–6.
23. Kanakis CD, Tarantilis PA, Tajmir-Riahi HA, et al. (2007a). Crocetin, dimethylcrocetin, and safranal bind human serum albumin:stability and antioxidative properties. J Agric Food Chem 55:970–7.
24. Kanakis CD, Tarantilis PA, Tajmir-Riahi HA, et al. (2007b). DNA interaction with saffron’s secondary metabolites safranal, crocetin, and dimethylcrocetin. DNA Cell Biol 26:63–70.
25. Koul A, Abraham SK., (2017). Efficacy of crocin and safranal as protective agents against genotoxic stress induced by gamma radiation, urethane and procarbazine in mice. Hum Exp Toxicol. In press. doi:10.1177/0960327116689715.
26. Lee JC, Kim HR, Kim J, et al. (2002). Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. Saboten. J Agric Food Chem 50:6490–6.
27. Lowry OH, Rosenbrough NJ, Farr AL, et al. (1951). Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–75.
28. Mehri S, Abnous K, Khooei A, et al. (2015). Crocin reduced acrylamide-induced neurotoxicity in wistar rat through inhibition of oxidative stress. Iran J Basic Med Sci 18:902–8.
29. Melnyk JP, Wang S, Marcone MF., (2010). Chemical and biological properties of the world's most expensive spice:Saffron. Food Res Int 43:1981–9.
30. Ohkawa H, Ohishi N, Yagi K., (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–8.
31. Paul P, Unnikrishnan MK, Nagappa AN., (2011). Phytochemicals as radioprotective agents–a review. Indian J Nat Prod Resour 2:137–50.
32. Pellegrini N, Serafini M, Salvatore S, et al. (2006). Total antioxidant capacity of spices, dried fruits, nuts, pulses, cereals and sweets consumed in Italy assessed by three different in vitro assays. Mol Nutr Food Res 50:1030–8.
33. Premkumar K, Abraham SK, Santhiya ST, et al. (2001). Inhibition of genotoxicity by saffron (Crocus sativus l.) in mice. Drug Chem Toxicol 24:421–8.
34. Premkumar K, Abraham SK, Santhiya ST, et al. (2003a). Inhibitory effects of aqueous crude extract of Saffron (Crocus sativus L.) on chemical-induced genotoxicity in mice. Asia Pacific J Clin Nutr 12:474–6.
35. Premkumar K, Abraham SK, Santhiya ST, et al. (2003b). Protective effects of saffron (Crocus sativus Linn.) on genotoxins-induced oxidative stress in Swiss albino mice. Phytother Res 17:614–17.
36. Razavi BM, Hosseinzadeh H., (2015). Saffron as an antidote or a protective agent against natural or chemical toxicities. DARU 23:31.
37. Razmaraii N, Babaei H, Mohajjel Nayebi A, et al. (2016). Crocin treatment prevents doxorubicin-induced cardiotoxicity in rats. Life Sci 157:145–51.
38. Roberts JC, Franceti DJ., (1993). The importance of sample preparation and storage in glutathione analysis. Anal Biochem 211:183–7.
39. Sadeghnia HR, Kamkar M, Assadpour E, et al. (2013). Protective effect of safranal, a constituent of crocus sativus, on quinolinic acid-induced oxidative damage in rat hippocampus. Iran J Basic Med Sci 16:73–82.
40. Salimi M, Mozdarani H, Nazari E., (2014). Cytogenetic alterations in preimplantation mice embryos following male mouse gonadal gamma-irradiation:comparison of two methods for reproductive toxicity screening. Avicenna J Med Biotechnol 6:130–9.
41. Samarth RM, Panwar M, Kumar M, et al. (2008). Evaluation of antioxidant and radical-scavenging activities of certain radioprotective plant extracts. Food Chem 106:868–73.
42. Schmid W., (1975). The micronucleus test. Mutat Res 31:9–15.
43. Weiss JF, Landauer MR., (2003). Protection against ionizing radiation by antioxidant nutrients and phytochemicals. Toxicology 189:1–20.