An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli

Biotechnology and Bioengineering

Volumn 114, Issue 7, 2017, Pages 1511-1520

  Theodosiou, Eleni ^a   Breisch, Marina ^b   Julsing, Mattijs K ^b   Falcioni, Francesco ^b   Bühler, Bruno ^a   Schmid, Andreas ^a  

^a HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^b TU DORTMUND UNIVERSITY   (Germany)

Author keywords

l proline transporter; proline hydroxylation; TCA cycle engineering; whole cell biocatalysis; KG dependent dioxygenase

Indexed keywords

AMINO ACIDS; CATALYSIS; ESCHERICHIA COLI; GENES; HYDROXYLATION; RESTORATION; STRAIN;

DIOXYGENASES; ENGINEERED ESCHERICHIA COLI; L-PROLINE; OXYFUNCTIONALIZATION; SELECTION PRESSURES; TCA CYCLE; WHOLE-CELL BIOCATALYSIS; WILD-TYPE STRAIN;

CELL ENGINEERING;

2 OXOGLUTARIC ACID; FUMARATE REDUCTASE; HYDROXYPROLINE; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; PROLINE; SUCCINYL COENZYME A; ALPHA-KETOGLUTARIC ACID; MIXED FUNCTION OXIDASE;

ACEA GENE; AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL CELL WALL; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BIOCATALYSIS; BIOENGINEERING; CELL FUNCTION; CELL SIZE; CITRIC ACID CYCLE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE DELETION; GENE OVEREXPRESSION; HYDROXYLATION; NONHUMAN; P4H GENE; PUTP GENE; SUCA GENE; SUCC GENE; BIOSYNTHESIS; CATALYSIS; GENE EXPRESSION REGULATION; GENETIC ENHANCEMENT; GENETICS; METABOLISM; PHYSIOLOGY; PROCEDURES; PROTEIN ENGINEERING;

CATALYSIS; CITRIC ACID CYCLE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC ENHANCEMENT; HYDROXYPROLINE; KETOGLUTARIC ACIDS; MIXED FUNCTION OXYGENASES; PROTEIN ENGINEERING;

EID: 85015370385     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26281     Document Type: Article

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