Effect of sucA or sucC gene knockout on the metabolism in Escherichia coli based on gene expressions, enzyme activities, intracellular metabolite concentrations and metabolic fluxes by 13C-labeling experiments

Biochemical Engineering Journal

Volumn 30, Issue 3, 2006, Pages 286-296

  Li, Mai ^a,b   Ho, Pei Yee ^a   Yao, Shanjing ^b   Shimizu, Kazuyuki ^a,c  

^a KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

^b ZHEJIANG UNIVERSITY   (China)

^c KEIO UNIVERSITY   (Japan)

Author keywords

13C Labeling experiment; Enzyme activity; Intracellular metabolite; Metabolic flux analysis; RT PCR; sucA gene knockout; sucC gene knockout

Indexed keywords

ENZYME KINETICS; GENETIC ENGINEERING; GLUCOSE; GROWTH KINETICS; METABOLITES; MUTAGENESIS;

13C-LABELING EXPERIMENT; ENZYME ACTIVITY; INTRACELLULAR METABOLITE; METABOLIC FLUX ANALYSIS; RT-PCR; SUCA GENE KNOCKOUT; SUCC GENE KNOCKOUT;

ESCHERICHIA COLI;

3 PHOSPHOFRUCTOSE KINASE; ACETATE KINASE; ACETIC ACID; CARBON 13; CITRATE SYNTHASE; FOCAL ADHESION KINASE; FRUCTOSE BISPHOSPHATE ALDOLASE; FUMARATE HYDRATASE; GLUCOSE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLUTAMIC ACID; GLYCERALDEHYDE 3 PHOSPHATE; GLYCOLYTIC ENZYME; GLYOXYLIC ACID; ISOCITRATE DEHYDROGENASE; ISOCITRATE LYASE; MALATE DEHYDROGENASE; MALATE DEHYDROGENASE (DECARBOXYLATING); MALATE SYNTHASE; PENTOSE PHOSPHATE; PHOSPHOFRUCTOSE KINASE; PHOSPHOGLUCONATE DEHYDROGENASE; PHOSPHOTRANSFERASE; TRICARBOXYLIC ACID; TRIOSEPHOSPHATE ISOMERASE; UNCLASSIFIED DRUG;

ACEA GENE; AEROBIC CELL CULTURE; ARCA GENE; ARTICLE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM MUTANT; CARBON NUCLEAR MAGNETIC RESONANCE; CELL GROWTH; CELL LEVEL; CITRIC ACID CYCLE; CONCENTRATION (PARAMETERS); DOWN REGULATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME MECHANISM; ESCHERICHIA COLI; EXPERIMENT; FADR GENE; FNR GENE; GAS CHROMATOGRAPHY; GENE; GENE EXPRESSION; GENE MUTATION; GLUCONEOGENESIS; GLUCOSE TRANSPORT; GLYCOLYSIS; GROWTH RATE; ICLR GENE; ISOTOPE LABELING; KNOCKOUT GENE; MASS SPECTROMETRY; METABOLITE; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; REGULATOR GENE; SUCA GENE; SUCC GENE; UPREGULATION;

ARCA; ESCHERICHIA COLI;

EID: 33745282141     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2006.05.011     Document Type: Article

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