Ser57 in the Na+/proline permease of Escherichia coli is critical for high-affinity proline uptake

European Journal of Biochemistry

Volumn 239, Issue 3, 1996, Pages 732-736

  Quick, Matthias ^a   Tebbe, Sandra ^a   Jung, Heinrich ^a  

^a UNIVERSITY OF OSNABRÜCK   (Germany)

Author keywords

PutP; Secondary transport; Site directed mutagenesis

Indexed keywords

3,4 DEHYDROPROLINE; AZETIDINE 2 CARBOXYLIC ACID; PERMEASE; PROLINE; PROLINE DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID TRANSPORT; ARTICLE; CONTROLLED STUDY; DRUG SENSITIVITY; ENZYME BINDING; ESCHERICHIA COLI; MUTANT; NONHUMAN; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0029957853     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0732u.x     Document Type: Article

References (35)

1. + (Li )-proline cotransport in Esccherichia coli, J. Member. Biol. 84, 157-164.
2. Chen, C.-C. & Wilson, T. H. (1986) Solubilization and functional reconstitution of the proline transport system of Escherichia coli, J. Biol. Chem. 261, 2599-2604.
3. Dila, K. D. & Maloy, S. R. (1986) Proline transport in Salmonella typhiinurium: putP permease mutants with altered substrate specificity, J. Bacteriol. 168, 590-594.
4. Hanada, K., Yamato, I. & Anraku, Y. (1985) Identification of proline carrier in Escherichia coli K-12, FEBS Lett. 191, 278-282.
5. Hanada, K., Yamato, I. & Anraku, Y. (1988) Purification and reconstitution of Escherichia coli proline carrier using a site specifically cleavable fusion protein, J. Biol. Chem. 263, 7181-7185.
6. +/proline symport carrier of Esc herichia coli, Biochim. Biophys. Acta 1105, 61-66.
7. Hattori, M. & Sakaki, Y. (1986) Dideoxy sequencing method using denatured plasmid templates, Anal. Biochem. 152, 232-238.
8. Hediger, M. A. (1994) Structure, function and evolution of solute transporters in prokaryotes and eukaryotes, J. Exp. Biol. 196, 15-49.
9. Jung, K., Jung, H., Wu, J., Privé, G. G. & Kaback, H. R. (1993) Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli, Biochemistry 32, 12273-12278.
10. Jung, H., Jung, K. & Kaback, H. R. (1994) A confomational change in the lactose permease of Escherichia coli is induced by ligand binding or membrane potential, Protein Sci. 3, 1053-1057.
11. Kabaek, H. R., Jung, K., Jung, H., Wu, J., Privé. G. G. & Zen, K. (1993) Helix packing in the C-terminal half of lactose permease, in Advances in cell and molecular biology of membranes and organelles (Dalbey, R. E., ed.) vol. 4, pp. 129-144, JAI Press, Inc.
12. +/proline symport carrier in Escherichia coli, FEBS Lett. 256, 135-138.
13. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
14. Miller, J. H. (1992) A short course in bacterial Genetics. A laboratory manual and handbook for Escherichia coli and related bacteria, Cold Spring Harbor Laboratory Press, Cold Sring Harbor, New York.
15. Mus-Veteau, I., Pourcher, T. & Leblanc, G. (1995) Melibiose permease of Escherichia coli: substrate-induced conformational changes monitored by tryptophan tluorescence spectroscopy. Biochemistry 34, 6775-6783.
16. Myers, R. S., Townsend, D. & Maloy, S. (1991) Dissecting the molecular mechanism of ion-solute cotransport: substrate specificity mutations in the putP gene affect the kinetics of proline transport, J. Membr. Biol. 121, 201-214.
17. Nakao, T., Yamato, I. & Anraku, Y. (1987) Nucleotide sequence of putP, the proline carrier gene of Escherichia coli K12, Mol. & Gen. Genet. 208, 70-75.
18. +-induced conformational change of the intestinal brush border sodium/glucose symporter using fluorescent probes. Biochemistry 26, 4272-4279.
19. Peterson, G. L. (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable, Anal. Biochem. 83, 346-356.
20. Poolman, B. & Konings, W. N. (1993) Secondary solute transport in bacteria. Biochem. Biophys. Acta 1183, 5-39.
21. Richardson, J. S. & Richardson, D. C. (1989) Principles and patterns of protein conformation, in Prediction of protein structure and the principles of protein conformation (Fasman, G. D., ed.) pp. 1-98, Plenum Press, New York.
22. Reizer, J., Reizer, A. & Saier, M. H. (1994) A functional superfamily of sodium/solute symporters. Biochim. Biophys. Acta 1197, 133-166.
23. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA 74, 5463-5467.
24. Spooner, P. J. R., Rutherford, N. G., Watts, A. & Henderson, P. J. F. (1994) NMR observation of substrate in the binding site of an active sugar-H′ symport protein in native membranes, Proc. Natl Acad. Sci. USA 91, 3877-3881.
25. Stalmuch, M. E., Grothe, S. & Wood, J. M. (1983) Two proline porters in Escherichia coli, J. Bacteriol. 156, 481-486.
26. + involvement in the proline transport in Escherichia coli, FEMS Microbiol. Lett. 198, 161-164.
27. Tabor, S. & Richardson, C. C. (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl Acad. Sci. USA 82, 1074-1078.
28. Tsuchia, T., Yamane, Y., Shiota, S. & Kawasaki, T. (1984) Cotransport of proline and Li in Escherichia coli, FEBS Lett. 168, 327 330.
29. Wood, J. M. & Zadworny, D. (1979) Characterization of an inducible porter required for L-proline catabolism by Escherichia coli K12, Can. J. Biochem. 57, 1328-1330.
30. Wood, J. M. & Zadworny, D. (1980) Amplification of the put genes and identification of the put gene products in Escherichia coli K 12, Can. J. Biochem. 58, 787-796.
31. Yamato, I. & Anraku, Y. (1988) Site-specific alteration of cysteine 281, cystcine 344, and cysteine 349 in the proline carrier of Escherichia coli, J. Biol. Chem. 263, 16055-16057.
32. +/proline symport carrier, J. Biol. Chem. 265, 2450-2455.
33. +/substrate symport in procaryotes, in Alkali cation transport systems in procaryotes (Bakker, E. P., ed.) pp. 53-76, CRC Press Inc., Boca Raton, Florida.
34. Yamato, I., Kotani, M., Oka, Y. & Anraku, Y. (1994) Site-specific alteration of arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning region of the proline carrier of Escherichia coli, J. Biol. Chem. 269, 5720-5724.
35. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985) Improved M 13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors, Gene (Amst.) 33, 103-119.