Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

Proceedings of the National Academy of Sciences of the United States of America

Volumn 114, Issue 10, 2017, Pages 2550-2555

  Terekhov, Stanislav S ^a,b   Smirnov, Ivan V ^a,c   Stepanova, Anastasiya V ^a   Bobik, Tatyana V ^a,c   Mokrushina, Yuliana A ^a   Ponomarenko, Natalia A ^a   Belogurov, Alexey A ^a,c   Rubtsova, Maria P ^b,d   Kartseva, Olga V ^c   Gomzikova, Marina O ^c   Moskovtsev, Alexey A ^e   Bukatin, Anton S ^f   Dubina, Michael V ^f   Kostryukova, Elena S ^g,h   Babenko, Vladislav V ^g   Vakhitova, Maria T ^h   Manolov, Alexander I ^g   Malakhova, Maja V ^g   Kornienko, Maria A ^g   Tyakht, Alexander V ^g,h   Vanyushkina, Anna A ^g   Ilina, Elena N ^g   Masson, Patrick ^c,i   Gabibov, Alexander G ^a,b,c   Altman, Sidney ^j   more..

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b MOSCOW STATE UNIVERSITY   (Russian Federation)

^c KAZAN FEDERAL UNIVERSITY   (Russian Federation)

^d SKOLKOVO INSTITUTE OF SCIENCE AND TECHNOLOGY   (Russian Federation)

^e INSTITUTE OF GENERAL PATHOLOGY AND PATHOPHYSIOLOGY   (Russian Federation)

^f ST PETERSBURG ACADEMIC UNIVERSITY   (Russian Federation)

^g FEDERAL RESEARCH AND CLINICAL CENTER OF PHYSICAL CHEMICAL MEDICINE OF FEDERAL MEDICAL BIOLOGICAL AGENCY   (Russian Federation)

^h MOSCOW INSTITUTE OF PHYSICS AND TECHNOLOGY   (Russian Federation)

ⁱ French Society of Clinical Cytology   (France)

^j YALE UNIVERSITY   (United States)

more..

Author keywords

Butyrylcholinesterase; Cell cell interactions; Microfluidic encapsulation; Staphylococcus aureus; Ultrahigh throughput screening

Indexed keywords

PARAOXON; CHOLINESTERASE; ESSENTIAL OIL; WATER;

ARTICLE; BACTERIUM IDENTIFICATION; BIOCATALYST; BIOCOMPATIBILITY; BIODIVERSITY; CELL INTERACTION; ENCAPSULATION; GENOTYPE PHENOTYPE CORRELATION; HIGH THROUGHPUT SCREENING; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; MICROFLUIDICS; MOUTH FLORA; NEXT GENERATION SEQUENCING; NONHUMAN; PREDICTIVE VALUE; PRIORITY JOURNAL; SINGLE CELL ANALYSIS; STAPHYLOCOCCUS AUREUS; ANTIBIOSIS; CELL COMMUNICATION; CHEMISTRY; DEVICES; DRUG EFFECTS; EMULSION; FLOW CYTOMETRY; GENOTYPE; GROWTH, DEVELOPMENT AND AGING; HIGH THROUGHPUT SEQUENCING; HUMAN; MICROFLUIDIC ANALYSIS; PHENOTYPE; PROCEDURES;

ANTIBIOSIS; BIODIVERSITY; BUTYRYLCHOLINESTERASE; CELL COMMUNICATION; EMULSIONS; FLOW CYTOMETRY; GENOTYPE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; OILS, VOLATILE; PARAOXON; PHENOTYPE; SINGLE-CELL ANALYSIS; STAPHYLOCOCCUS AUREUS; WATER;

EID: 85014656468     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1621226114     Document Type: Article

References (30)

1. Agresti JJ, et al. (2010) Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proc Natl Acad Sci USA 107(9):4004-4009.
2. Zhang H, et al. (2016) Autocrine-based selection of drugs that target ion channels from combinatorial venom peptide libraries. Angewandte Chemie International Edition 55(32):9306-9310.
3. Gupta RD, et al. (2011) Directed evolution of hydrolases for prevention of G-type nerve agent intoxication. Nat Chem Biol 7(2):120-125.
4. Aharoni A, Amitai G, Bernath K, Magdassi S, Tawfik DS (2005) High-throughput screening of enzyme libraries: Thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. Chem Biol 12(12):1281-1289.
5. Tawfik DS, Griffiths AD (1998) Man-made cell-like compartments for molecular evolution. Nat Biotechnol 16(7):652-656.
6. Miller OJ, et al. (2006) Directed evolution by in vitro compartmentalization. Nat Methods 3(7):561-570.
7. Najah M, et al. (2014) Droplet-based microfluidics platform for ultra-high-throughput bioprospecting of cellulolytic microorganisms. Chem Biol 21(12):1722-1732.
8. Yan J, et al. (2013) Monodisperse water-in-oil-in-water (W/O/W) double emulsion droplets as uniform compartments for high-throughput analysis via flow cytometry. Micromachines (Basel) 4(4):402-413.
9. Zinchenko A, et al. (2014) One in a million: Flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution. Anal Chem 86(5):2526-2533.
10. Zhang Y, et al. (2013) A programmable microenvironment for cellular studies via microfluidics-generated double emulsions. Biomaterials 34(19):4564-4572.
11. Masson P, Lockridge O (2010) Butyrylcholinesterase for protection from organophosphorus poisons: Catalytic complexities and hysteretic behavior. Arch Biochem Biophys 494(2):107-120.
12. Geyer BC, et al. (2010) Plant-derived human butyrylcholinesterase, but not an organophosphorous-compound hydrolyzing variant thereof, protects rodents against nerve agents. Proc Natl Acad Sci USA 107(47):20251-20256.
13. Ilyushin DG, et al. (2013) Chemical polysialylation of human recombinant butyrylcholinesterase delivers a long-acting bioscavenger for nerve agents in vivo. Proc Natl Acad Sci USA 110(4):1243-1248.
14. Kelsic ED, Zhao J, Vetsigian K, Kishony R (2015) Counteraction of antibiotic production and degradation stabilizes microbial communities. Nature 521(7553):516-519.
15. Tenconi E, Guichard P, Motte P, Matagne A, Rigali S (2013) Use of red autofluorescence for monitoring prodiginine biosynthesis. J Microbiol Methods 93(2):138-143.
16. Lopez-Garcia MT, Rioseras B, Yague P, Alvarez JR, Manteca A (2014) Cell immobilization of Streptomyces coelicolor: Effect on differentiation and actinorhodin production. Int Microbiol 17(2):75-80.
17. Luker KE, et al. (2015) Comparative study reveals better far-red fluorescent protein for whole body imaging. Sci Rep 5:10332.
18. Sawyer AJ, et al. (2013) A peptide-morpholino oligomer conjugate targeting Staphylococcus aureus gyrA mRNA improves healing in an infected mouse cutaneous wound model. Int J Pharm 453(2):651-655.
19. Smith AJ, Jackson MS, Bagg J (2001) The ecology of Staphylococcus species in the oral cavity. J Med Microbiol 50(11):940-946.
20. Shim JU, et al. (2013) Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays. ACS Nano 7(7):5955-5964.
21. McDonald JC, et al. (2000) Fabrication of microfluidic systems in poly(dimethylsiloxane). Electrophoresis 21(1):27-40.
22. Mazutis L, et al. (2013) Single-cell analysis and sorting using droplet-based microfluidics. Nat Protoc 8(5):870-891.
23. Teh S-Y, Khnouf R, Fan H, Lee AP (2011) Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics. Biomicrofluidics 5(4):044113.
24. Terekhov S, et al. (2015) A novel expression cassette delivers efficient production of exclusively tetrameric human butyrylcholinesterase with improved pharmacokinetics for protection against organophosphate poisoning. Biochimie 118:51-59.
25. Gasparian ME, et al. (2013) Heterogeneous catalysis on the phage surface: Display of active human enteropeptidase. Biochimie 95(11):2076-2081.
26. Wu S, Letchworth GJ (2004) High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. Biotechniques 36(1):152-154.
27. Ralph EC, Xiang L, Cashman JR, Zhang J (2011) His-tag truncated butyrylcholinesterase as a useful construct for in vitro characterization of wild-type and variant butyrylcholinesterases. Protein Expr Purif 80(1):22-27.
28. Lõoke M, Kristjuhan K, Kristjuhan A (2011) Extraction of genomic DNA from yeasts for PCR-based applications. Biotechniques 50(5):325-328.
29. Kitz R, Wilson IB (1962) Esters of methanesulfonic acid as irreversible inhibitors of acetylcholinesterase. J Biol Chem 237(10):3245-3249.
30. Ellman GL, Courtney KD, Andres V, Jr, Feather-Stone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7(2):88-95.