Two-colour fluorescence fluorimetric analysis for direct quantification of bacteria and its application in monitoring bacterial growth in cellulose degradation systems

Journal of Microbiological Methods

Volumn 135, Issue , 2017, Pages 85-92

  Duedu, Kwabena O ^a,b   French, Christopher E ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF HEALTH AND ALLIED SCIENCES   (Ghana)

Author keywords

Cell density; Cellulose; Fluorimetry; Propidium iodide; Quantification of bacteria; SYBR Green

Indexed keywords

BACTERIAL DNA; CELLULOSE; DYE; PROPIDIUM IODIDE; SYBR I; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; FLUORESCENT DYE; NUCLEIC ACID; ORGANIC COMPOUND; SYBR GREEN I; WASTE WATER;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIUM DETECTION; BACTERIUM QUANTIFICATION; CONTROLLED STUDY; CULTURE MEDIUM; DEGRADATION; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; MICROPLATE READER; NONHUMAN; REPRODUCIBILITY; BACTERIAL COUNT; BACTERIUM; COLOR; DRUG EFFECTS; ENERGY TRANSFER; ENVIRONMENTAL MONITORING; ENZYMOLOGY; FLUORESCENCE; FLUOROMETRY; FOOD CONTROL; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MICROBIOLOGY; PROCEDURES; SPECTROPHOTOMETRY; STAINING; WASTE WATER;

BACTERIA; BACTERIAL PROTEINS; CELLULOSE; COLONY COUNT, MICROBIAL; COLOR; DNA, BACTERIAL; ENERGY TRANSFER; ENVIRONMENTAL MONITORING; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENT DYES; FLUOROMETRY; FOOD MICROBIOLOGY; NUCLEIC ACIDS; ORGANIC CHEMICALS; PROPIDIUM; SPECTROPHOTOMETRY; STAINING AND LABELING; WASTE WATER;

EID: 85013634311     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2017.02.006     Document Type: Article

References (42)

1. Barbesti, S., Citterio, S., Labra, M., Baroni, M.D., Neri, M.G., Sgorbati, S., Two and three-color fluorescence flow cytometric analysis of immunoidentified viable bacteria. Cytometry 40 (2000), 214–218.
2. Breed, R.S., Dotterrer, W.D., The number of colonies allowable on satisfactory agar plates. J. Bacteriol. 1 (1916), 321–331.
3. Caron, G.N.-V., Badley, R.A., Viability assessment of bacteria in mixed populations using flow cytometry. J. Microsc. 179 (1995), 55–66.
4. Creutzig, F., Ravindranath, N.H., Berndes, G., Bolwig, S., Bright, R., Cherubini, F., Chum, H., Corbera, E., Delucchi, M., Faaij, A., Fargione, J., Haberl, H., Heath, G., Lucon, O., Plevin, R., Popp, A., Robledo-Abad, C., Rose, S., Smith, P., Stromman, A., Suh, S., Masera, O., Bioenergy and climate change mitigation: an assessment. GCB Bioenergy 7 (2015), 916–944.
5. Diaper, J.P., Tither, K., Edwards, C., Rapid assessment of bacterial viability by flow cytometry. Appl. Microbiol. Biotechnol. 38 (1992), 268–272.
6. Duedu, K.O., French, C.E., Characterization of a Cellulomonas fimi exoglucanase/xylanase-endoglucanase gene fusion which improves microbial degradation of cellulosic biomass. Enzym. Microb. Technol. 93–94 (2016), 113–121.
7. Duedu, K.O., French, C.E., Discriminating dead/live bacteria in homogenous cell suspensions and the effect of insoluble substrates on turbidimetric measurements. Data Brief, 2017 (Submitted).
8. Foladori, P., Bruni, L., Tamburini, S., Ziglio, G., Direct quantification of bacterial biomass in influent, effluent and activated sludge of wastewater treatment plants by using flow cytometry. Water Res. 44 (2010), 3807–3818.
9. French, C.E., Synthetic biology and biomass conversion: a match made in heaven?. J. R. Soc. Interface 6:Suppl. 4 (2009), S547–S558.
10. French, C.E., Horsfall, L., Barnard, D.K., Duedu, K., Fletcher, E., Joshi, N., Kane, S.D., Lakhundi, S.S., Liu, C.-K., Oltmanns, J., Radford, D., Salinas, A., White, J., Elfick, A., Beyond genetic engineering: technical capabilities in the application fields of biocatalysis and biosensors. Giese, B., Pade, C., Wigger, H., von Gleich, A., (eds.) Synthetic Biology, 2015, Springer International Publishing, 113–137.
11. Fulton, L.M., Lynd, L.R., Körner, A., Greene, N., Tonachel, L.R., The need for biofuels as part of a low carbon energy future. Biofuels Bioprod. Biorefin. 9 (2015), 476–483.
12. Gaigalas, A.K., Li, L., Marti, G., Henderson, O., Vogt, R., Barr, J., The development of fluorescence intensity standards. J. Res. Natl. Inst. Stand. Technol. 106 (2001), 381–389.
13. Gratama, J.W., D'Hautcourt, J.-L., Mandy, F., Rothe, G., Barnett, D., Janossy, G., Papa, S., Schmitz, G., Lenkei, R., The European Working Group on Clinical Cell, A, Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. Cytometry 33 (1998), 166–178.
14. Gregori, G., Citterio, S., Ghiani, A., Labra, M., Sgorbati, S., Brown, S., Denis, M., Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining. Appl. Environ. Microbiol. 67 (2001), 4662–4670.
15. Himmel, M.E., Ding, S.Y., Johnson, D.K., Adney, W.S., Nimlos, M.R., Brady, J.W., Foust, T.D., Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315 (2007), 804–807.
16. Humphreys, M.J., Allman, R., Lloyd, D., Determination of the viability of trichomonas vaginalis using flow cytometry. Cytometry 15 (1994), 343–348.
17. Kaprelyants, A.S., Kell, D.B., Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry. J. Appl. Bacteriol. 72 (1992), 410–422.
18. Koch, A.L., Turbidity measurements of bacterial cultures in some available commercial instruments. Anal. Biochem. 38 (1970), 252–259.
19. Lakhundi, S.S., Duedu, K.O., Cain, N., Nagy, R., Krakowiak, J., French, C.E., Citrobacter freundii as a test platform for recombinant cellulose degradation systems. Lett. Appl. Microbiol. 64 (2017), 35–42.
20. Lebaron, P., Parthuisot, N., Catala, P., Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems. Appl. Environ. Microbiol. 64 (1998), 1725–1730.
21. Lenkei, R., Gratama, J.W., Rothe, G., Schmitz, G., D'Hautcourt, J.L., Årekrans, A., Mandy, F., Marti, G., Performance of calibration standards for antigen quantitation with flow cytometry. Cytometry 33 (1998), 188–196.
22. Lenkei, R., Mandy, F., Marti, G., Vogt, R., Quantitative fluorescence cytometry: an emerging consensus. Cytometry, 1998, John Wiley & Sons, Inc.
23. Melamed, M.R., Adams, L.R., Zimring, A., Murnick, J.G., Mayer, K., Preliminary evaluation of acridine orange as a vital stain for automatic differential leukocyte counts. Am. J. Clin. Pathol. 57 (1972), 95–102.
24. Nuffield Council on Bioethics, Biofuels: Ethical Issues. 2011, Nuffield Council on Bioethics, Abingdon.
25. Nunez, R., Garay, N., Villafane, C., Bruno, A., Lindgren, V., Description of a flow cytometry approach based on SYBR-14 staining for the assessment of DNA content, cell cycle analysis, and sorting of living normal and neoplastic cells. Exp. Mol. Pathol. 76 (2004), 29–36.
26. Porter, J., Deere, D., Pickup, R., Edwards, C., Fluorescent probes and flow cytometry: new insights into environmental bacteriology. Cytometry 23 (1996), 91–96.
27. Purvis, N., Stelzer, G., Multi-platform, multi-site instrumentation and reagent standardization. Cytometry 33 (1998), 156–165.
28. Schwartz, A., Gaigalas, A.K., Wang, L., Marti, G.E., Vogt, R.F., Fernandez-Repollet, E., Formalization of the MESF unit of fluorescence intensity. Cytometry B Clin. Cytom. 57B (2004), 1–6.
29. Schwartz, A., Marti, G.E., Poon, R., Gratama, J.W., Fernández-Repollet, E., Standardizing flow cytometry: a classification system of fluorescence standards used for flow cytometry. Cytometry 33 (1998), 106–114.
30. Schwartz, A., Wang, L., Early, E.A., Gaigalas, A.K., Zhang, A., Marti, G.E., Vogt, R.F., Quantitating fluorescence intensity from fluorophore: the definition of MESF assignment. J. Res. Natl. Inst. Stand. Technol. 107 (2002), 83–91.
31. Sgorbati, S., Barbesti, S., Citterio, S., Bestetti, G., De Veechi, R., Characterization of number, DNA content, viability and cell size of bacteria from natural environments using DAPI/PI dual staining and flow cytometry. Minerva Biotech. 8 (1996), 9–15.
32. Shapiro, H.M., Perlmutter, N.G., Stein, P.G., A flow cytometer designed for fluorescence calibration. Cytometry 33 (1998), 280–287.
33. Tamburini, S., Foladori, P., Ferrentino, G., Spilimbergo, S., Jousson, O., Accurate flow cytometric monitoring of Escherichia coli subpopulations on solid food treated with high pressure carbon dioxide. J. Appl. Microbiol. 117 (2014), 440–450.
34. Treuer, R., Haydel, S.E., Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension. Curr. Protoc. Microbiol., 2011 (Chapter 10, Unit 10A 16).
35. US EPA, Renewable Fuel Standards (RFS). 2013.
36. Van Dyk, J.S., Pletschke, B.I., A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes—factors affecting enzymes, conversion and synergy. Biotechnol. Adv. 30 (2012), 1458–1480.
37. Wang, L.L., Gaigalas, A.K., Schwartz, A., Abbasi, F., Vogt, R.F., Quantitating fluorescence intensity from fluorophores: practical use of MESF values. J. Res. Natl. Inst. Stand. Technol. 107 (2002), 339–353.
38. Waxdal, M.J., Monical, M.C., Palini, A.G., Inter-laboratory relative fluorescence intensity measurements using FlowCal 575 calibration beads: a baseline study. Cytometry 33 (1998), 213–218.
39. Wood, J.C.S., Fundamental flow cytometer properties governing sensitivity and resolution. Cytometry 33 (1998), 260–266.
40. Wood, J.C.S., Hoffman, R.A., Evaluating fluorescence sensitivity on flow cytometers: an overview. Cytometry 33 (1998), 256–259.
41. Zenger, V.E., Vogt, R., Mandy, F., Schwartz, A., Marti, G.E., Quantitative flow cytometry: inter-laboratory variation. Cytometry 33 (1998), 138–145.
42. Zhang, Y.-z., Kemper, C., Bakke, A., Haugland, R.P., Novel flow cytometry compensation standards: internally stained fluorescent microspheres with matched emission spectra and long-term stability. Cytometry 33 (1998), 244–248.