Direct quantification of bacterial biomass in influent, effluent and activated sludge of wastewater treatment plants by using flow cytometry

Water Research

Volumn 44, Issue 13, 2010, Pages 3807-3818

  Foladori, P ^a   Bruni, L ^b   Tamburini, S ^a   Ziglio, G ^a  

^a UNIVERSITY OF TRENTO   (Italy)

^b Agenzia per la Depurazione   (Italy)

Author keywords

Activated sludge; Activity; Bacterial biomass; Bacterial biovolume; Flow cytometry; Viability; Wastewater

Indexed keywords

ACTIVATED SLUDGE PROCESS; BIOMASS; CHEMICAL OXYGEN DEMAND; EFFLUENT TREATMENT; FLOW CYTOMETRY; RECLAMATION; SEWAGE PUMPING PLANTS; TOXICITY; WASTEWATER TREATMENT; WATER TREATMENT PLANTS;

ACTIVATED SLUDGE; ACTIVITY; BACTERIAL BIOMASS; BACTERIAL BIOVOLUME; BACTERIAL CELLS; BIOVOLUMES; PARTICULATE CHEMICAL OXYGEN DEMANDS; RAW WASTEWATERS; VIABILITY; WASTE WATER TREATMENT PLANTS;

EFFLUENTS;

ACTIVATED SLUDGE; BIOMASS; CHEMICAL OXYGEN DEMAND; EFFLUENT; ENZYME ACTIVITY; FLOW MODELING; MICROBIAL ACTIVITY; PERMEABILITY; QUANTITATIVE ANALYSIS; VOLATILE SUBSTANCE; WASTEWATER; WATER TREATMENT;

ACTIVATED SLUDGE; ARTICLE; BACTERIAL VIABILITY; EFFLUENT; EPIFLUORESCENCE MICROSCOPY; FILTRATION; FLOW CYTOMETRY; MICROBIAL BIOMASS; PARTICULATE MATTER; PRIORITY JOURNAL; ULTRASOUND; WASTE WATER MANAGEMENT; WASTE WATER TREATMENT PLANT; BACTERIA; BIOMASS; CYTOLOGY; DEVICES; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MICROBIAL VIABILITY; MICROBIOLOGY; PROCEDURES; SEWAGE; WATER MANAGEMENT;

BACTERIA (MICROORGANISMS);

OXYGEN; SEWAGE;

BACTERIA; BIOMASS; FILTRATION; FLOW CYTOMETRY; MICROBIAL VIABILITY; OXYGEN; SEWAGE; SONICATION; WASTE DISPOSAL, FLUID; WATER PURIFICATION;

EID: 77953812080     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.watres.2010.04.027     Document Type: Article

References (32)

1. Andreottola G., Foladori P., Gelmini A., Ziglio G. Biomass active fraction evaluated by a direct method and respirometric techniques. Water Science and Technology 2002, 46(1-2):371-379.
2. APHA, AWWA, WPCF. Standard Methods for the Examination of Water and Wastewater, 19th Edn.; American Public Health Association/American Water Works Association/Water Environment Federation: Washington DC, USA, 1995.
3. Banks C.J., Walker I. Sonication of activated sludge flocs and the recovery of their bacteria on solid media. Journal of General Microbiology 1977, 98:363-368.
4. Bergquist P.L., Hardiman E.M., Ferrari B.C., Wisley T. Applications of flow cytometry in environmental microbiology and biotechnology. Extremophiles 2009, 13:389-401.
5. Biggs C.A., Lant P.A. Activated sludge flocculation: on-line determination of floc size and the effect of shear. Water Research 2000, 34(9):2542-2550.
6. Corno G., Caravati E., Callieri C., Bertoni R. Effects of predation pressure on bacterial abundance, diversity and size-structure distribution in a oligotrophic system. Journal of Limnology 2008, 67(2):107-119.
7. Ekama G.A., Marais G.v.R., Siebritz I.P., Pitman A.R., Keay G.F.P., Buchan L., Gerber A., Smollen M. Theory, Design and Operation of Nutrient Removal Activated Sludge Processes 1984, Water Research Commission of South Africa, Pretoria.
8. Falcioni T., Manti A., Boi P., Canonico B., Balsamo M., Papa S. Comparison of disruption procedure for enumeration of activated sludge floc bacteria by flow cytometry. Cytometry Part B 2006, 70B:149-153.
9. Foladori P., Bruni L., Andreottola G., Ziglio G. Effects of sonication on bacteria viability in wastewater treatment plants evaluated by flow cytometry-fecal indicators, wastewater and activated sludge. Water Research 2007, 41:235-243.
10. Foladori P., Quaranta A., Ziglio G. Use of silica microspheres having refractive index similar to bacteria for conversion of flow cytometric forward light scatter in biovolume. Water Research 2008, 42(14):3757-3766.
11. Frølund B., Palmgren R., Keiding K., Nielsen P.H. Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Research 1996, 30:1749-1758.
12. Fry J.C. Direct methods and biomass estimation. Methods Microbiol 1990, 22:41-85.
13. Gilbride K.A., Lee D.-Y., Beaudette L.A. Molecular techniques in wastewater: understanding microbial communities, detecting pathogens, and real-time process control. Journal of Microbiological Methods 2006, 66:1-20.
14. Henze, et al. Activated sludge models ASM1, ASM2, ASM2d and ASM3. Scientific and Technical Report No. 9 2000, IWA Publishing, London, UK. IWA Task Group on Mathematical Modelling for Design and Operation of Biological Wastewater Treatment (Ed.).
15. Jenkins D., Richard M.G., Daigger G.T. Manual on the Causes and Control of Activated Sludge Bulking and Foaming 1993, Lewis Publishers, Inc., Chelsea, Michigan, 48118 pp. second ed.
16. Jorand F., Zartarian F., Thomas F., Block J., Bottero J.Y., Villemin G., Urbain V., Manem J. Chemical and structural (2D) linkage between bacteria within activated sludge flocs. Water Research 1995, 29(7):1639-1647.
17. Jürgens K., Pernthaler J., Schalla S., Amann R. Morphological and compositional changes in a planktonic bacterial community in response to enhanced protozoan grazing. Applied and Environmental Microbiology 1999, 65(3):1241-1250.
18. Kappeler J., Gujer W. Estimation of kinetic parameters of heterotrophic biomass under aerobic conditions and characterisation of wastewater for activated sludge modelling. Water and Science Technology 1992, 25(6):125-139.
19. Mason T.J., Lorimer J.P., Bates D.M. Quantifying sonochemistry: casting some light on a 'black art'. Ultrasonics 1992, 30(1):40-42.
20. Münch E., Pollard P.C. Measuring bacterial biomass-COD in wastewater containing particulate matter. Water Research 1997, 31(10):2550-2556.
21. Nebe-von-Caron G., Stephens P.J., Hewitt C.J., Powell J.R., Badley R.A. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. Journal of Microbiological Methods 2000, 42:97-114.
22. 12FDG as substrates for β-galactosidase detection by flow cytometry in animal, bacterial and yeast cells. Applied and Environmental Microbiology 1994, 60(12):4638-4641.
23. Porter J., Deere D., Hardman M., Edwards C., Pickup R. Go with the flow - use of flow cytometry in environmental microbiology. FEMS Microbiology Ecology 1997, 24:93-101.
24. Spérandio M., Urbain V., Ginestet P., Audic M.J., Paul E. Application of COD fractionation by a new combined technique: comparison of various wastewaters and sources of variability. Water Science and Technology 2001, 43(1):181-190.
25. Steen H.B. Flow cytometry of bacteria: glimpses from the past with a view to the future. Journal of Microbiological Methods 2000, 42:65-74.
26. Vives-Rego J., Lebaron P., Nebe-von Caron G. Current and future applications of flow cytometry in aquatic microbiology. FEMS Microbiology Reviews 2000, 24:429-448.
27. Vollertsen J., Jahn A., Nielsen J.L., Hvitved-Jacobsen T., Nielsen P.H. Comparison of methods for determination of microbial biomass in wastewater. Water Research 2001, 35:1649-1658.
28. Wagner M., Amann R., Lemmer H., Schleifer K.H. Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure. Applied and Environmental Microbiology 1993, 59(5):1520-1525.
29. Wentzel M.C., Ubisi M.F., Ekama G.A. Heterotrophic active biomass component of activated sludge mixed liquor. Water Science and Technology 1998, 37(4/5):79-87.
30. Williams A.R., Forster C.F., Hughes D.E. Using an ultrasonic technique in the enumeration of activated sludge. Effluent and Water Treatment Journal 1971, 11:83-86.
31. Zhang P., Zhang G., Wang W. Ultrasonic treatment of biological sludge: floc disintegration, cell lysis and inactivation. Bioresource Technology 2007, 98:207-210.
32. Ziglio G., Andreottola G., Barbesti S., Boschetti G., Bruni L., Foladori P., Villa R. Assessment of activated sludge viability with flow cytometry. Water Research 2002, 36:460-468.