Comparison of blue nuclei acid dyes for flow cytometric enumeration of bacteria in aquatic systems

Applied and Environmental Microbiology

Volumn 64, Issue 5, 1998, Pages 1725-1730

  Lebaron, Philippe ^a   Parthuisot, Nathalie ^a   Catala, Philippe ^a  

^a LABORATOIRE ARAGO   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DYE; FRESH WATER; NUCLEIC ACID; SEA WATER; SYBR I; SYBR II; SYTO 11; SYTO 13; SYTO 16; SYTO 9; SYTO BC; UNCLASSIFIED DRUG; BACTERIAL DNA; BACTERIAL RNA; COLORING AGENT;

ARTICLE; BACTERIUM ISOLATION; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; NONHUMAN; STAINING; BACTERIAL COUNT; COMPARATIVE STUDY; MICROBIOLOGY;

BACTERIA (MICROORGANISMS);

COLONY COUNT, MICROBIAL; COLORING AGENTS; DNA, BACTERIAL; FLOW CYTOMETRY; RNA, BACTERIAL; STAINING AND LABELING; WATER MICROBIOLOGY;

EID: 0031596935     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.5.1725-1730.1998     Document Type: Article

References (20)

1. Alfreider, A., J. Pernthaler, R. Amann, B. Sattler, F.-O. Glöckner, A. Wille, and R. Psenner. 1996. Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization. Appl. Environ. Microbiol. 62:2138-2144.
2. Boye, M., T. Ahl, and S. Molin. 1995. Application of a strain-specific rRNA oligonucleotide probe targeting Pseudomonas fluorescens Ag1 in a mesocosm study of bacterial release into the environment. Appl. Environ. Microbiol. 61:1384-1390.
3. Bremer, H., and P. P. Dennis. 1987. Modulation of chemical composition and other parameters of the cell by growth rate, p. 1527-1542. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
4. del Giorgio, P. A., D. F. Bird, Y. T. Prairie, and D. Planas. 1996. Row cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13. Limnol. Oceanogr. 41:783-789.
5. Jeffrey, W. H., R. V. Haven, M. P. Hoch, and R. B. Coffin. 1996. Bacterioplankton RNA. DNA, protein content and relationships to rates of thymidine and leucine incorporation. Aquat. Microb. Ecol. 10:87-95.
6. Joux, F., P. Lebaron, and M. Troussellier. 1997. Succession of cellular states in a Salmonella typhimurium population submitted to starvation in artificial seawater microcosms. FEMS Microbiol. Ecol. 22:65-76.
7. Karner, M., and J. A. Fuhrman. 1997. Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl. Environ. Microbiol. 63:1208-1213.
8. Kemp, P. F., S. Lee, and J. LaRoche. 1993. Estimating the growth rate of slowly growing marine bacteria from RNA content. Appl. Environ. Microbiol. 59:2594-2601.
9. Kramer, J. G., and F. L. Singleton. 1993. Measurement of rRNA variations in natural communities of microorganisms on the southeastern U.S. continental shelf. Appl. Environ. Microbiol. 59:2430-2436.
10. Lebaron, P., M. Troussellier, and P. Got. 1993. Accuracy and precision of epifluorescence microscopy count for direct estimates of bacterial numbers. J. Microbiol. Methods 19:89-94.
11. Lebaron, P., P. Catala, C. Fajon, F. Joux, J. Baudart, and L. Bernard. 1997. A new sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligonucleotide probe targeting rRNA and tyramide signal amplification. Appl. Environ. Microbiol. 63:3274-3278.
12. Li, W. K. W., J. F. Jellett, and P. M. Dickie. 1995. DNA distributions in planktonic bacteria stained with TOTO or TO-PRO. Limnol. Oceanogr. 40:1485-1495.
13. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
14. Marie, D., D. Vaulot, and F. Partensky. 1996. Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes. Appl. Environ. Microbiol. 62:1649-1655.
15. Marie, D., F. Partensky, S. Jacquet, and D. Vaulot. 1997. Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Appl. Environ. Microbiol. 63:186-193.
16. McFeters, G. A., F. P. Yu, B. H. Pyle, and P. S. Stewart. 1995. Physiological assessment of bacteria using fluorochromes. J. Microbiol. Methods 21:1-13.
17. Ouverney, C., and J. A. Furhman. 1997. Increase in fluorescence intensity of 16S rRNA in situ hybridization in natural samples treated with chloramphenicol. Appl. Environ. Microbiol. 63:2735-2740.
18. Porter, J., D. Deere, R. Pickup, and C. Edwards. 1996. Fluorescent probes and flow cytometry: new insights into environmental bacteriology. Cytometry 23:91-96.
19. Troussellier, M., C. Courties, and A. Vaquer. 1993. Recent applications of flow cytometry in aquatic microbial ecology. Biol. Cell 78:111-121.
20. Wallner, G., R. I. Amann, and W. Beisker. 1993. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14:136-143.