Two and three-color fluorescence flow cytometric analysis of immunoidentified viable bacteria

Cytometry

Volumn 40, Issue 3, 2000, Pages 214-218

  Barbesti, Silvia ^a   Citterio, Sandra ^a   Labra, Massimo ^a   Baroni, Maurizio Davide ^a   Neri, Maria Grazia ^a   Sgorbati, Sergio ^a,b  

^a UNIVERSITY OF MILAN   (Italy)

^b Sezione di Botanica Generale   (Italy)

Author keywords

Bacteria; Environmental microbiology; Flow cytometry; Industrial; Propidium iodide; RPE Cy5; Sanitary; SYBR Green I; Viable bacteria immunodetection

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; BIOTIN; FLUOROCHROME; POLYCLONAL ANTIBODY;

ANTIBODY LABELING; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; CELL VIABILITY; COLOR; DIAGNOSTIC VALUE; FLOW CYTOMETRY; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; BACILLUS SUBTILIS; BACTERIOLOGICAL TECHNIQUES; ENVIRONMENTAL MICROBIOLOGY; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENT DYES; HUMANS; ORGANIC CHEMICALS; PROPIDIUM; RABBITS; STAINING AND LABELING;

BACTERIA (MICROORGANISMS); ORYCTOLAGUS CUNICULUS;

EID: 0033935288     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20000701)40:3<214::AID-CYTO6>3.0.CO;2-M     Document Type: Article

References (16)

1. Xu HS, Roberts N, Singleton FL, Attwell RW, Grimes DJ, Colwell RR. Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb Ecol 1982;8:313-323.
2. Amann RI, Wolfgang L, Shleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 1995;59:143-169.
3. Kaprelyants AS, Kell DB. Rapid assessment of bacterial viability and vitality by Rhodamine 123 and flow cytometry. J Appl Bacteriol 1992;72:410-422.
4. Porter J, Deere D, Pickup R, Edwards C. Fluorescent probes and flow cytometry: new insight into environmental microbiology. Cytometry 1996;23:91-96.
5. Diaper JP, Tither K, Edwards C. Rapid assessment of bacteria viability by flow cytometry. Appl Microbiol Biotechnol 1992;38:268-272.
6. Nebe-Von Caron G, Badley RA. Viability assessment of bacteria in mixed populations using flow cytometry. J Microsc 1995;179:55-66.
7. Sgorbati S, Barhesti S, Citterio S, Citterio S, De Vecchi R. Characterization of number, DNA content, viability and cell size of bacteria from natural environments using DAP1/P1 dual staining and flow cytometry. Minerva Biotec 1996;8:9-15.
8. Humphreys MJ, Allman R, Lloyd D. Determination of the viability of Trichomonas vaginalis using flow cytometry. Cytometry 1994;15: 343-348.
9. Williams SC, Hong Y, Danavall DCA, Howard-Jones MH, Gibson D, Frisher ME, Verity PG. Distinguishing between living and non living bacteria: evaluation of the vital stain propiditim iodide and its combined tise with molecular probes in aquatic samples. J Microbiol Methods 1998;32:225-236.
10. Bunthof CJ, Van den Braak S, Breeuwer P, Rombouts FM, Abee T. Rapid fluorescence assessment of the viability of stressed Lactobacillus lactis. Appl Environ Microbiol 1999;65:3681-3689.
11. Haugland RP. Handbook of fluorescent probes and research chemicals, sixth edition. Eugene, OR: Molecular Probes; 1996. 680 p.
12. Marie D, Partensky F, Jacquet S, Vaulot D. Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow citometry using the nucleic acid stain SYBR Green 1. Appl Environ Microbiol 1997;63:186-193.
13. Lebaron P, Parthuisot N, Catala P. Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems. Appl Environ Microbiol 1998;64:1725-1730.
14. Gasol JM, Zweifel UL, Peters F, Fuhrman JA, Hagstrom A. Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria. Appl Environ Microbiol 1999;65:4475-4483.
15. Sgorbati S, Harbesti S, Neri MG, Davegna C, Citterio S. Rapid flow cytometric immunodetection of bacteria to monitor aquatic environments. Eur J Histochem 1997;41:187-188.
16. Bratten JL. Nuclei acid stain for cells and more. In: Bratten JL, editor. BioProbes 31. Eugene, OR: Molecular Probes; 1999. p 2.