Accurate quantitation of circulating cell-free mitochondrial DNA in plasma by droplet digital PCR

Analytical and Bioanalytical Chemistry

Volumn 409, Issue 10, 2017, Pages 2727-2735

  Ye, Wei ^a   Tang, Xiaojun ^a   Liu, Chu ^a   Wen, Chaowei ^a   Li, Wei ^a   Lyu, Jianxin ^a  

^a WENZHOU MEDICAL UNIVERSITY   (China)

Author keywords

Circulating cell free DNA; Droplet digital PCR; Mitochondrial DNA

Indexed keywords

CYTOLOGY; DNA; DROPS; EXTRACTION; LANTHANUM COMPOUNDS; MITOCHONDRIA;

CELL-FREE; DETECTING PLASMAS; DIGITAL PCR; DNA EXTRACTION; HIGH SENSITIVITY; MITOCHONDRIAL DNA; PLASMA SAMPLES; SENSITIVITY AND SPECIFICITY;

POLYMERASE CHAIN REACTION;

CELL FREE NUCLEIC ACID; MITOCHONDRIAL DNA;

ADULT; BLOOD; CHEMISTRY; FEASIBILITY STUDY; FEMALE; GENETICS; HUMAN; MALE; MITOCHONDRION; NORMAL HUMAN; PLASMA; POLYMERASE CHAIN REACTION; PROCEDURES; YOUNG ADULT;

ADULT; CELL-FREE NUCLEIC ACIDS; DNA, MITOCHONDRIAL; FEASIBILITY STUDIES; FEMALE; HEALTHY VOLUNTEERS; HUMANS; MALE; MITOCHONDRIA; PLASMA; POLYMERASE CHAIN REACTION; YOUNG ADULT;

EID: 85011706143     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-017-0217-x     Document Type: Article

References (34)

1. Mandel P, Metais P. C R Seances Soc Biol Fil. 1948;142(3–4):241–3. Les acides nucleiques du plasma sanguin chez l’homme.
2. Leon SA, Shapiro B, Sklaroff DM, Yaros MJ. Free DNA in the serum of cancer patients and the effect of therapy. Cancer Res. 1977;37(3):646–50.
3. Thakur BK, Zhang H, Becker A, Matei I, Huang Y, Costa-Silva B, et al. Double-stranded DNA in exosomes: a novel biomarker in cancer detection. Cell Res. 2014;24(6):766–9.
4. Yu M. Circulating cell-free mitochondrial DNA as a novel cancer biomarker: opportunities and challenges. Mitochondrial DNA. 2012;23(5):329–32.
5. Huang CY, Chen YM, Wu CH, Tsai CM, Lee YC, Perng RP, et al. Circulating free mitochondrial DNA concentration and its association with erlotinib treatment in patients with adenocarcinoma of the lung. Oncol Lett. 2014;7(6):2180–4.
6. Ellinger J, Muller DC, Muller SC, Hauser S, Heukamp LC, von Ruecker A, et al. Circulating mitochondrial DNA in serum: a universal diagnostic biomarker for patients with urological malignancies. Urol Oncol. 2012;30(4):509–15.
7. Fernandes J, Michel V, Camorlinga-Ponce M, Gomez A, Maldonado C, De Reuse H, et al. Circulating mitochondrial DNA level, a noninvasive biomarker for the early detection of gastric cancer. Cancer Epidemiol Biomarkers Prev. 2014;23(11):2430–8.
8. McIlroy DJ, Bigland M, White AE, Hardy BM, Lott N, Smith DW, et al. Cell necrosis-independent sustained mitochondrial and nuclear DNA release following trauma surgery. J Trauma Acute Care Surg. 2015;78(2):282.
9. Zhang Q, Raoof M, Chen Y, Sumi Y, Sursal T, Junger W, et al. Circulating mitochondrial DAMPs cause inflammatory responses to injury. Nature. 2010;464(7285):104–7.
10. Lee HK, Song JH, Shin CS, Park DJ, Park KS, Lee KU, et al. Decreased mitochondrial DNA content in peripheral blood precedes the development of non-insulin-dependent diabetes mellitus. Diabetes Res Clin Pract. 1998;42(3):161–7.
11. Nakahira K, Kyung SY, Rogers AJ, Gazourian L, Youn S, Massaro AF, et al. Circulating mitochondrial DNA in patients in the ICU as a marker of mortality: derivation and validation. PLoS Med. 2013;10(12):e1001577. discussion e.
12. Devonshire AS, Whale AS, Gutteridge A, Jones G, Cowen S, Foy CA, et al. Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification. Anal Bioanal Chem. 2014;406(26):6499–512.
13. Ajaz S, Czajka A, Malik A. Accurate measurement of circulating mitochondrial DNA content from human blood samples using real-time quantitative PCR. Methods Mol Biol. 2015;1264:117–31.
14. Manoj P. Droplet digital PCR technology promises new applications and research areas. Mitochondrial DNA. 2014.
15. Hudecova I. Digital PCR, analysis of circulating nucleic acids. Clin Biochem. 2015.
16. Rebolledo-Jaramillo B, Su MS, Stoler N, McElhoe JA, Dickins B, Blankenberg D, et al. Maternal age effect and severe germ-line bottleneck in the inheritance of human mitochondrial DNA. Proc Natl Acad Sci U S A. 2014;111(43):15474–9.
17. Taylor SD, Ericson NG, Burton JN, Prolla TA, Silber JR, Shendure J, et al. Targeted enrichment and high-resolution digital profiling of mitochondrial DNA deletions in human brain. Aging Cell. 2014;13(1):29–38.
18. Podlesniy P, Figueiro-Silva J, Llado A, Antonell A, Sanchez-Valle R, Alcolea D, et al. Low cerebrospinal fluid concentration of mitochondrial DNA in preclinical Alzheimer disease. Ann Neurol. 2013;74(5):655–68.
19. Sedlak RH, Cook L, Cheng A, Magaret A, Jerome KR. Clinical utility of droplet digital PCR for human cytomegalovirus. J Clin Microbiol. 2014;52(8):2844–8.
20. Dingle TC, Sedlak RH, Cook L, Jerome KR. Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances. Clin Chem. 2013;59(11):1670–2.
21. Chiu RW, Chan LY, Lam NY, Tsui NB, Ng EK, Rainer TH, et al. Quantitative analysis of circulating mitochondrial DNA in plasma. Clin Chem. 2003;49(5):719–26.
22. Pan BT, Teng K, Wu C, Adam M, Johnstone RM. Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes. J Cell Biol. 1985;101(3):942–8.
23. Thery C, Zitvogel L, Amigorena S. Exosomes: composition, biogenesis and function. Nat Rev Immunol. 2002;2(8):569–79.
24. Keller S, Sanderson MP, Stoeck A, Altevogt P. Exosomes: from biogenesis and secretion to biological function. Immunol Lett. 2006;107(2):102–8.
25. Loyer X, Vion AC, Tedgui A, Boulanger CM. Microvesicles as cell-cell messengers in cardiovascular diseases. Circ Res. 2014;114(2):345–53.
26. Guescini M, Genedani S, Stocchi V, Agnati LF. Astrocytes and Glioblastoma cells release exosomes carrying mtDNA. J Neural Transm. 2010;117(1):1–4.
27. Guescini M, Guidolin D, Vallorani L, Casadei L, Gioacchini AM, Tibollo P, et al. C2C12 myoblasts release micro-vesicles containing mtDNA and proteins involved in signal transduction. Exp Cell Res. 2010;316(12):1977–84.
28. Helmig S, Fruhbeis C, Kramer-Albers EM, Simon P, Tug S. Release of bulk cell free DNA during physical exercise occurs independent of extracellular vesicles. Eur J Appl Physiol. 2015;115(11):2271–80.
29. Lobb RJ, Becker M, Wen SW, Wong CS, Wiegmans AP, Leimgruber A, et al. Optimized exosome isolation protocol for cell culture supernatant and human plasma. J Extracell Vesicles. 2015;4:27031.
30. Beck J, Bierau S, Balzer S, Andag R, Kanzow P, Schmitz J, et al. Digital droplet PCR for rapid quantification of donor DNA in the circulation of transplant recipients as a potential universal biomarker of graft injury. Clin Chem. 2013;59(12):1732–41.
31. Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, et al. Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for “inflamm-aging”. Eur J Immunol. 2014;44(5):1552–62.
32. Jahr S, Hentze H, Englisch S, Hardt D, Fackelmayer FO, Hesch RD, et al. DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells. Cancer Res. 2001;61(4):1659–65.
33. Zhou Y, Xu H, Xu W, Wang B, Wu H, Tao Y, et al. Exosomes released by human umbilical cord mesenchymal stem cells protect against cisplatin-induced renal oxidative stress and apoptosis in vivo and in vitro. Stem Cell Res Ther. 2013;4(2):34.
34. Sahoo S, Losordo DW. Exosomes and cardiac repair after myocardial infarction. Circ Res. 2014;114(2):333–44.