Pooled CRISPR screening with single-cell transcriptome readout

Nature Methods

Volumn 14, Issue 3, 2017, Pages 297-301

  Datlinger, Paul ^a   Rendeiro, André F ^a   Schmidl, Christian ^a   Krausgruber, Thomas ^a   Traxler, Peter ^a   Klughammer, Johanna ^a   Schuster, Linda C ^a   Kuchler, Amelie ^a   Alpar, Donat ^a   Bock, Christoph ^a,b,c  

^a ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^b MEDICAL UNIVERSITY OF VIENNA   (Austria)

^c MAX PLANCK INSTITUTE FOR INFORMATICS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GUIDE RNA; MESSENGER RNA; PROTEIN KINASE LCK; PROTEIN KINASE ZAP 70; PUROMYCIN; RNA POLYMERASE II; T LYMPHOCYTE RECEPTOR; TRANSCRIPTOME;

AMPLICON; ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; FLOW CYTOMETRY; GENE EDITING; GENE EXPRESSION; HUMAN; LONG TERMINAL REPEAT; NEXT GENERATION SEQUENCING; PRIORITY JOURNAL; PROTEIN DEPLETION; REVERSE TRANSCRIPTION; RNA SEQUENCE; RNA TRANSCRIPTION; CELL LINE; CELL PROLIFERATION; GENE EXPRESSION PROFILING; GENETICS; HEK293 CELL LINE; HIGH THROUGHPUT SEQUENCING; PROCEDURES; SEQUENCE ANALYSIS; SINGLE CELL ANALYSIS;

CELL LINE; CELL PROLIFERATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENE EXPRESSION PROFILING; HEK293 CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; RNA, GUIDE; SEQUENCE ANALYSIS, RNA; SINGLE-CELL ANALYSIS; TRANSCRIPTOME;

EID: 85010898455     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.4177     Document Type: Article

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