Subtilisin QK-2: Secretory expression in Lactococcus lactis and surface display onto gram-positive enhancer matrix (GEM) particles

Microbial Cell Factories

Volumn 15, Issue 1, 2016, Pages

  Mao, Ruifeng ^a   Zhou, Kangping ^a   Han, Zhenwei ^a   Wang, Yefu ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Gram positive enhancer matrix; Lactic acid bacteria; Secretory expression; Subtilisin QK 2; Surface display; Thrombus

Indexed keywords

ALKALINE PROTEINASE; BACTERIAL PROTEIN; GRAM POSITIVE ENHANCER MATRIX; SIGNAL PEPTIDE; SUBTILISIN; SUBTILISIN QK 2; UNCLASSIFIED DRUG; CODON; HYBRID PROTEIN;

ACMA GENE; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; CARBOXY TERMINAL SEQUENCE; CELL SURFACE DISPLAY; CODON; EXPRESSION VECTOR; FIBRINOLYSIS; FIBRINOLYTIC THERAPY; GENE SEQUENCE; GENETIC TRANSFORMATION; IMMUNOFLUORESCENCE MICROSCOPY; LACTIC ACID BACTERIUM; LACTOCOCCUS LACTIS; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; QKPRO GENE; SEQUENCE ALIGNMENT; STOMACH JUICE; SUPERNATANT; WESTERN BLOTTING; XBAI GENE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PH;

BLOTTING, WESTERN; CODON; FIBRINOLYSIS; GENE EXPRESSION REGULATION, BACTERIAL; HYDROGEN-ION CONCENTRATION; LACTOCOCCUS LACTIS; MICROSCOPY, FLUORESCENCE; PLASMIDS; PROMOTER REGIONS, GENETIC; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; SUBTILISIN;

EID: 85007420531     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0478-7     Document Type: Article

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