Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhEs)

Metabolic Engineering Communications

Volumn 3, Issue , 2016, Pages 120-129

  Hon, Shuen ^a,b   Lanahan, Anthony A ^a,b   Tian, Liang ^a,b   Giannone, Richard J ^b,c   Hettich, Robert L ^b,c   Olson, Daniel G ^a,b   Lynd, Lee R ^a,b  

^a DARTMOUTH COLLEGE   (United States)

^b BioEnergy Science Center   (United States)

^c OAK RIDGE NATIONAL LABORATORY   (United States)

Author keywords

adhE; Clostridium Thermocellum; Gene expression; Plasmid; Structural stability

Indexed keywords

ALCOHOL DEHYDROGENASE; ALDEHYDE;

ALCOHOL PRODUCTION; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CLOSTRIDIUM THERMOCELLUM; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEOMICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SANGER SEQUENCING; WHOLE GENOME SEQUENCING;

EID: 84997226657     PISSN: None     EISSN: 22140301     Source Type: Journal    
DOI: 10.1016/j.meteno.2016.04.001     Document Type: Article

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