Transformation of clostridium thermocellum by electroporation

Methods in Enzymology

Volumn 510, Issue , 2012, Pages 317-330

  Olson, Daniel G ^a,d   Lynd, Lee R ^a,b,c,d  

^a DARTMOUTH COLLEGE   (United States)

^b Mascoma Corporation   (United States)

^c BioEnergy Science Center ^*  (United States)

^d BioEnergy Science Center   (United States)

Author keywords

Clostridium thermocellum; CTFUD; electroporation; electrotransformation; genetic system; transformation

Indexed keywords

8 AZAHYPOXANTHINE DERIVATIVE; BACTERIAL DNA; FLOXURIDINE; FLUOROOROTIC ACID; NEOMYCIN; THIAMPHENICOL; URACIL;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM TRANSFORMATION; CELL GROWTH; CLOSTRIDIUM THERMOCELLUM; DNA FLANKING REGION; ELECTROPORATION; GENE DELETION; GENE INSERTION; KARYOTYPE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

CLOSTRIDIUM THERMOCELLUM;

EID: 84861156874     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-415931-0.00017-3     Document Type: Chapter

References (14)

1. D.A. Argyros, S.A. Tripathi, T.F. Barrett, S.R. Rogers, L.F. Feinberg, D.G. Olson, J.M. Foden, B.B. Miller, L.R. Lynd, D.A. Hogsett, and N.C. Caiazza High ethanol titers from cellulose using metabolically engineered thermophilic, anaerobic microbes Appl. Environ. Microbiol. 77 2011 8288 8294
2. D.C. Chang, B.M. Chassy, and S. Saunders Guide to Electroporation and Electrofusion 1992 Academic Press San Diego, CA
3. A.L. Demain, M. Newcomb, and J.H.D. Wu Cellulase, clostridia, and ethanol Microbiol. Mol. Biol. Rev. 69 2005 124 154
4. W.J. Dower, J.F. Miller, and C.W. Ragsdale High efficiency transformation of E. coli by high voltage electroporation Nucleic Acids Res. 16 1988 6127 6145
5. E.A. Johnson, A. Madia, and A.L. Demain Chemically defined minimal medium for growth of the anaerobic cellulolytic thermophile Clostridium thermocellum Appl. Environ. Microbiol. 41 1981 1060 1062
6. R. Lamed, E. Setter, and E.A. Bayer Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum J. Bacteriol. 156 1983 828 836
7. T. Maniatis, E.F. Fritsch, and J. Sambrook Molecular Cloning: A Laboratory Manual 1982 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
8. D.G. Olson Electrotransformation of Gram-positive, Anaerobic, Thermophilic Bacteria W.I.P. Orginazation, 2009 Mascoma Corporation Geneva, Switzerland
9. D.G. Olson Genetic investigations of the Clostridium thermocellum cellulosome 2011 Thayer School of Engineering, Ph.D., Dartmouth College, Hanover, NH, 2011, p. 118
10. D.G. Olson, S.A. Tripathi, R.J. Giannone, J. Lo, N.C. Caiazza, D.A. Hogsett, R.L. Hettich, A.M. Guss, G. Dubrovsky, and L.R. Lynd Deletion of the Cel48S cellulase from Clostridium thermocellum Proc. Natl. Acad. Sci. USA 107 2010 17727 17732
11. R.M.Q. Shanks, N.C. Caiazza, S.M. Hinsa, C.M. Toutain, and G.A. O'Toole Saccharomyces cerevisiae-based molecular tool kit for manipulation of genes from gram-negative bacteria Appl. Environ. Microbiol. 72 2006 5027 5036
12. R.M.Q. Shanks, D.E. Kadouri, D.P. MacEachran, and G.A. O'Toole New yeast recombineering tools for bacteria Plasmid 62 2009 88 97
13. S.A. Tripathi, D.G. Olson, D.A. Argyros, B.B. Miller, T.F. Barrett, D.M. Murphy, J.D. McCool, A.K. Warner, V.B. Rajgarhia, L.R. Lynd, D.A. Hogsett, and N.C. Caiazza Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant Appl. Environ. Microbiol. 76 2010 6591 6599
14. M.V. Tyurin, S.G. Desai, and L.R. Lynd Electrotransformation of Clostridium thermocellum Appl. Environ. Microbiol. 65 2004 883 890