Role of the CipA scaffoldin protein in cellulose solubilization, as determined by targeted gene deletion and complementation in Clostridium thermocellum

Journal of Bacteriology

Volumn 195, Issue 4, 2013, Pages 733-739

  Olson, Daniel G ^a,d   Giannone, Richard J ^b,d   Hettich, Robert L ^b,d   Lynd, Lee R ^a,b,c,d  

^a DARTMOUTH COLLEGE   (United States)

^b OAK RIDGE NATIONAL LABORATORY   (United States)

^c Mascoma Corporation   (United States)

^d BioEnergy Science Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULASE; CELLULOSOME; CIPA PROTEIN; SCAFFOLD PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; CLOSTRIDIUM THERMOCELLUM; DELETION MUTANT; ENZYME ACTIVITY; GENE DELETION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; SOLUBILIZATION; START CODON; TANDEM MASS SPECTROMETRY;

BACTERIAL PROTEINS; CELLULASE; CELLULOSE; CHROMOSOME MAPPING; CHROMOSOMES, BACTERIAL; CLOSTRIDIUM THERMOCELLUM; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; MEMBRANE PROTEINS; TANDEM MASS SPECTROMETRY;

EID: 84873530148     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.02014-12     Document Type: Article

References (33)

1. Demain AL, Newcomb M, Wu JHD. 2005. Cellulase, clostridia, and ethanol. Microbiol. Mol. Biol. Rev. 69:124-154.
2. Lamed R, Setter E, Bayer EA. 1983. Characterization of a cellulosebinding, cellulase-containing complex in Clostridium thermocellum. J. Bacteriol. 156:828-836.
3. Wu JHD, Orme-Johnson WH, Demain AL. 1988. Two components of an extracellular protein aggregate of Clostridium thermocellum together degrade crystalline cellulose. Biochemistry 27:1703-1709.
4. Gerngross UT, Romaniec MPM, Kobayashi T, Huskisson NS, Demain AL. 1993. Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal S(L) protein reveals an unusual degree of internal homology. Mol. Microbiol. 8:325-334.
5. Tokatlidis K, Salamitou S, Beguin P, Dhurjati P, Aubert JP. 1991. Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components. FEBS Lett. 291:185-188.
6. Lemaire M, Ohayon H, Gounon P, Fujino T, Beguin P. 1995. OlpB, a new outer layer protein of Clostridium thermocellum, and binding of its S-layer-like domains to components of the cell-envelope. J. Bacteriol. 177: 2451-2459.
7. Adams JJ, Pal G, Jia Z, Smith SP. 2006. Mechanism of bacterial cellsurface attachment revealed by the structure of cellulosomal type II cohesin- dockerin complex. Proc. Natl. Acad. Sci. U. S. A. 103:305-310.
8. Bayer EA, Setter E, Lamed R. 1985. Organization and distribution of the cellulosome in Clostridium thermocellum. J. Bacteriol. 163:552-559.
9. Bayer EA, Shimon LJW, Shoham Y, Lamed R. 1998. Cellulosomes-structure and ultrastructure. J. Struct. Biol. 124:221-234.
10. Bayer EA, Lamed R. 1986. Ultrastructure of the cell-surface cellulosome of Clostridium thermocellum and its interaction with cellulose. J. Bacteriol. 167:828-836.
11. Bayer EA, Kenig R, Lamed R. 1983. Adherence of Clostridium thermocellum to cellulose. J. Bacteriol. 156:818-827.
12. Lamed R, Naimark J, Morgenstern E, Bayer EA. 1987. Specialized cell-surface structures in cellulolytic bacteria. J. Bacteriol. 169:3792-3800.
13. Zverlov VV, Klupp M, Krauss J, Schwarz WH. 2008. Mutations in the scaffoldin gene, cipA, of Clostridium thermocellum with impaired cellulosome formation and cellulose hydrolysis: insertions of a new transposable element, IS1447, and implications for cellulase synergism on crystalline cellulose. J. Bacteriol. 190:4321-4327.
14. Olson DG, Tripathi SA, Giannone RJ, Lo J, Caiazza NC, Hogsett DA, Hettich RL, Guss AM, Dubrovsky G, Lynd LR. 2010. Deletion of the Cel48S cellulase from Clostridium thermocellum. Proc. Natl. Acad. Sci. U. S. A. 107:17727-17732.
15. Argyros DA, Tripathi SA, Barrett TF, Rogers SR, Feinberg LF, Olson DG, Foden JM, Miller BB, Lynd LR, Hogsett DA, Caiazza NC. 2011. High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes Appl. Environ. Microbiol. 77:8288-8294.
16. Shanks RMQ, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA. 2006. Saccharomyces cerevisiae-based molecular tool kit for manipulation of genes from gram-negative bacteria. Appl. Environ. Microbiol. 72:5027-5036.
17. Maniatis T, Fritsch EF, Sambrook J. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
18. Tripathi SA, Olson DG, Argyros DA, Miller BB, Barrett TF, Murphy DM, McCool JD, Warner AK, Rajgarhia VB, Lynd LR, Hogsett DA, Caiazza NC. 2010. Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant. Appl. Environ. Microbiol. 76:6591-6599.
19. Guzman LM, Belin D, Carson MJ, Beckwith J. 1995. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177:4121-4130.
20. Waller B, Olson DG, Currie DH, Guss A, Lynd LR. 2013. Exchange of type II dockerin-containing subunits of the Clostridium thermocellum cellulosome. FEMS Microbiol. Lett. 338:46-53.
21. Olson DG, Lynd LR. 2012. Transformation of Clostridium thermocellum by electroporation. Methods Enzymol. 510:317-330.
22. Hogsett DA. 1995. Cellulose hydrolysis and fermentation by Clostridium thermocellum for the production of ethanol. Ph.D. thesis. Dartmouth College, Hanover, NH.
23. Ellis LD. 2011. Mass balances applied to cellulosic fermentations by Clostridium thermocellum (ATCC 27405) and the development of an automatic sampling system. M.S. thesis. Dartmouth College, Hanover, NH.
24. Zhang Y-HP, Lynd LR. 2005. Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum. 187:99-106.
25. Tabb DL, Fernando CG, Chambers MC. 2007. MyriMatch: highly accurate tandem mass spectral peptide identification by multivariate hypergeometric analysis. J. Proteome Res. 6:654-661.
26. Ma ZQ, Dasari S, Chambers MC, Litton MD, Sobecki SM, Zimmerman LJ, Halvey PJ, Schilling B, Drake PM, Gibson BW, Tabb DL. 2009. IDPicker 2.0: improved protein assembly with high discrimination peptide identification filtering. J. Proteome Res. 8:3872-3881.
27. Lochner A, Giannone RJ, Keller M, Antranikian G, Graham DE, Hettich RL. 2011. Label-free quantitative proteomics for the extremely thermophilic bacterium Caldicellulosiruptor obsidiansis reveal distinct abundance patterns upon growth on cellobiose, crystalline cellulose, and switchgrass. J. Proteome Res. 10:5302-5314.
28. Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B. 2009. The Carbohydrate-Active EnZymes database (CAZy): an expert resource for glycogenomics. Nucleic Acids Res. 37:233-238.
29. Finn RD, Mistry J, Tate J, Coggill P, Heger A, Pollington JE, Gavin OL, Gunasekaran P, Ceric G, Forslund K, Holm L, Sonnhammer ELL, Eddy SR, Bateman A. 2010. The Pfam protein families database. Nucleic Acids Res. 38:D211-D222.
30. Richards FJ. 1959. A flexible growth function for empirical use. J. Exp. Bot. 10:290-301.
31. Berger E, Zhang D, Zverlov VV, Schwarz WH. 2007. Two noncellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, hydrolyse crystalline cellulose synergistically. FEMS Microbiol. Lett. 268:194-201.
32. Desvaux M. 2005. The cellulosome of Clostridium cellulolyticum. Enzyme. Microb. Technol. 37:373-385.
33. Dror TW, Rolider A, Bayer EA, Lamed R, Shoham Y. 2003. Regulation of expression of scaffoldin-related genes in Clostridium thermocellum. J. Bacteriol. 185:5109-5116.