Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum

Biotechnology for Biofuels

Volumn 8, Issue 1, 2015, Pages

  Biswas, Ranjita ^a,c   Zheng, Tianyong ^a,b   Olson, Daniel G ^a,b   Lynd, Lee R ^a,b   Guss, Adam M ^a,d  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

^b DARTMOUTH COLLEGE   (United States)

^c INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^d NONE   (United States)

Author keywords

Cellulosic ethanol; Clostridium thermocellum; Hydrogenase maturation; Metabolic engineering

Indexed keywords

AMINO ACIDS; CARBON; CELLULOSIC ETHANOL; CLOSTRIDIUM; METABOLIC ENGINEERING;

ALCOHOL DEHYDROGENASE ACTIVITY; BIOFUEL PRODUCTION; CLOSTRIDIUM THERMOCELLUM; CONSOLIDATED BIO-PROCESSING; ETHANOL PRODUCTION; HYDROGENASE MATURATION; POST-TRANSLATIONAL MODIFICATIONS; [FEFE]HYDROGENASES;

ETHANOL;

AMINO ACID; BIOFUEL; ENZYME ACTIVITY; ETHANOL; MATURATION; METABOLISM; PROTEIN; REDOX CONDITIONS;

CLOSTRIDIUM THERMOCELLUM;

EID: 84924023248     PISSN: 17546834     EISSN: None     Source Type: Journal    
DOI: 10.1186/s13068-015-0204-4     Document Type: Article

References (33)

1. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev. 2002;66:506-77.
2. US Department of Energy. US billion-ton update: biomass supply for a bioenergy and bioproducts industry. Oak Ridge, TN: Oak Ridge National Laboratory; 2011.
3. Blumer-Schuette SE, Brown SD, Sander KB, Bayer EA, Kataeva I, Zurawski JV, et al. Thermophilic lignocellulose deconstruction. FEMS Microbiol Rev. 2014;38:393-448.
4. Lynd LR, Laser MS, Bransby D, Dale BE, Davison B, Hamilton R, et al. How biotech can transform biofuels. Nat Biotechnol. 2008;26:169-72.
5. Ellis LD, Holwerda EK, Hogsett D, Rogers S, Shao X, Tschaplinski T, et al. Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405). Bioresource Technol. 2012;103:293-9.
6. Shao X, Raman B, Zhu M, Mielenz JR, Brown SD, Guss AM, et al. Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum. Appl Microbiol Biotechnol. 2011;92:641-52.
7. Williams TI, Combs JC, Lynn BC, Strobel HJ. Proteomic profile changes in membranes of ethanol-tolerant Clostridium thermocellum. Appl Microbiol Biotechnol. 2007;74:422-32.
8. Argyros DA, Tripathi SA, Barrett TF, Rogers SR, Feinberg LF, Olson DG, et al. High ethanol titers from cellulose using metabolically engineered thermophilic, anaerobic microbes. Appl Environ Microbiol. 2011;77:8288-94.
9. Guss AM, Olson DG, Caiazza NC, Lynd LR. Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum. Biotechnol Biofuels. 2012;5:30.
10. Olson DG, Lynd LR. Transformation of Clostridium thermocellum by electroporation. Methods Enzymol. 2012;510:317-30.
11. Tripathi SA, Olson DG, Argyros DA, Miller BB, Barrett TF, Murphy DM, et al. Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant. Appl Environ Microbiol. 2010;76:6591-9.
12. Mohr G, Hong W, Zhang J, Cui GZ, Yang Y, Cui Q, et al. A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum. PLoS One. 2013;8:e69032.
13. Brown SD, Guss AM, Karpinets TV, Parks JM, Smolin N, Yang S, et al. Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum. Proc Natl Acad Sci U S A. 2011;108:13752-7.
14. Biswas R, Prabhu S, Lynd LR, Guss AM. Increase in ethanol yield via elimination of lactate production in an ethanol-tolerant mutant of Clostridium thermocellum. PLoS One. 2014;9:e86389.
15. Rydzak T, McQueen PD, Krokhin OV, Spicer V, Ezzati P, Dwivedi RC, et al. Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression. BMC Microbiol. 2012;12:214.
16. Deng Y, Olson DG, Zhou J, Herring CD, Joe Shaw A, Lynd LR. Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum. Metab Eng. 2013;15:151-8.
17. Raman B, McKeown CK, Rodriguez Jr M, Brown SD, Mielenz JR. Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation. BMC Microbiol. 2011;11:134.
18. Calusinska M, Happe T, Joris B, Wilmotte A. The surprising diversity of clostridial hydrogenases: a comparative genomic perspective. Microbiol-Sgm. 2010;156:1575-88.
19. Feinberg L, Foden J, Barrett T, Davenport KW, Bruce D, Detter C, et al. Complete genome sequence of the cellulolytic thermophile Clostridium thermocellum DSM1313. J Bacteriol. 2011;193:2906-7.
20. Mulder DW, Shepard EM, Meuser JE, Joshi N, King PW, Posewitz MC, et al. Insights into [FeFe]-hydrogenase structure, mechanism, and maturation. Structure. 2011;19:1038-52.
21. van der Veen D, Lo J, Brown SD, Johnson CM, Tschaplinski TJ, Martin M, et al. Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways. J Ind Microbiol Biotechnol. 2013;40:725-34.
22. Caffrey SM, Park HS, Voordouw JK, He Z, Zhou J, Voordouw G. Function of periplasmic hydrogenases in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough. J Bacteriol. 2007;189:6159-67.
23. Casalot L, De Luca G, Dermoun Z, Rousset M, de Philip P. Evidence for a fourth hydrogenase in Desulfovibrio fructosovorans. J Bacteriol. 2002;184:853-6.
24. Casalot L, Valette O, De Luca G, Dermoun Z, Rousset M, de Philip P. Construction and physiological studies of hydrogenase depleted mutants of Desulfovibrio fructosovorans. FEMS Microbiol Lett. 2002;214:107-12.
25. Rydzak T, Grigoryan M, Cunningham ZJ, Krokhin OV, Ezzati P, Cicek N, et al. Insights into electron flux through manipulation of fermentation conditions and assessment of protein expression profiles in Clostridium thermocellum. Appl Microbiol Biotechnol. 2014;98:6497-510.
26. Ozkan M, Yilmaz EI, Lynd LR, Ozcengiz G. Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization. Can J Microbiol. 2004;50:845-51.
27. Bryant FO. Characterization of the fructose 1,6-bisphosphate-activated, L(+)-lactate dehydrogenase from Thermoanaerobacter ethanolicus. J Enzyme Inhib. 1991;5:235-48.
28. Willquist K, van Niel EW. Lactate formation in Caldicellulosiruptor saccharolyticus is regulated by the energy carriers pyrophosphate and ATP. Metab Eng. 2010;12:282-90.
29. Wang S, Huang H, Moll J, Thauer RK. NADP+ reduction with reduced ferredoxin and NADP+ reduction with NADH are coupled via an electron-bifurcating enzyme complex in Clostridium kluyveri. J Bacteriol. 2010;192:5115-23.
30. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1982.
31. Shanks RMQ, Kadouri DE, MacEachran DP, O'Toole GA. New yeast recombineering tools for bacteria. Plasmid. 2009;62:88-97.
32. Hogsett D. Cellulose hydrolysis and fermentation by Clostridium thermocellum for the production of ethanol [PhD thesis]. Hanover, NH: Dartmouth College, Thayer School of Engineering; 1995.
33. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.